Institution
Children's Hospital Oakland Research Institute
About: Children's Hospital Oakland Research Institute is a based out in . It is known for research contribution in the topics: Population & Human leukocyte antigen. The organization has 1568 authors who have published 2480 publications receiving 203418 citations.
Topics: Population, Human leukocyte antigen, Haplotype, Gene, Cholesterol
Papers published on a yearly basis
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TL;DR: It is concluded that cellular vitamin C is a biological regulator of CFTR-mediated Cl secretion in epithelia and a potential nutraceutical and pharmaceutical target for the complementary treatment of sticky airway secretions by enhancing epithelial surface fluid secretion.
Abstract: Vitamin C (l-ascorbate) is present in the respiratory lining fluid of human lungs, and local deficits occur during oxidative stress. Here we report a unique function of vitamin C on the cystic fibrosis (CF) transmembrane conductance regulator (CFTR), a cAMP-dependent Cl channel that regulates epithelial surface fluid secretion. Vitamin C (100 μM) induced the openings of CFTR Cl channels by increasing its average open probability from 0 to 0.21 ± 0.08, without a detectable increase in intracellular cAMP levels. Exposure of the apical airway surface to vitamin C stimulated the transepithelial Cl secretion to 68% of forskolin-stimulated currents. The average half-maximal stimulatory constant was 36.5 ± 2.9 μM, which corresponds to physiological concentrations. When vitamin C was instilled into the nasal epithelium of human subjects, it effectively activated Cl transport in vivo. In CF epithelia, previous treatment of the underlying trafficking defect with trimethylamine oxide or expression of WT CFTR restored the activation of Cl transport by vitamin C. Sodium dependency and phloretin sensitivity, as well as the expression of transcripts for sodium-dependent vitamin C transporter (SVCT)-1 and SVCT2, support a model in which an apical vitamin C transporter is central for relaying the effect of vitamin C to CFTR. We conclude that cellular vitamin C is a biological regulator of CFTR-mediated Cl secretion in epithelia. The pool of vitamin C in the respiratory tract represents a potential nutraceutical and pharmaceutical target for the complementary treatment of sticky airway secretions by enhancing epithelial fluid secretion.
89 citations
TL;DR: It is documented that significant pools of NADPH and NADH are tightly bound to proteins and not detectable by many of the assay systems previously used, which results in a significant change in not only total RBC pyridine nucleotide content but also in the ratio of reduced to nonreduced nucleotide.
88 citations
George Washington University1, Northwestern University2, Boston Children's Hospital3, University of Pennsylvania4, University of Utah5, Emory University6, Johns Hopkins University7, Wayne State University8, Washington University in St. Louis9, University of Colorado Denver10, University of Pittsburgh11, Children's Hospital Oakland Research Institute12, Ohio State University13, University of Missouri–Kansas City14
TL;DR: Within this large contemporary cohort of newborns with perinatal HIE, the application of therapeutic hypothermia and associated neurodiagnostic studies appear to have expanded relative to reported clinical trials.
Abstract: Short-term outcomes after perinatal hypoxic ischemic encephalopathy: a report from the Children’s Hospitals Neonatal Consortium HIE focus group
88 citations
TL;DR: A single candidate 4′-phosphopantetheine transferase, identified by BLAST searches of the human genome sequence data base, has been cloned, expressed, and characterized and appears to utilize a single, broad specificity enzyme for all posttrans lysine catabolism reactions.
88 citations
TL;DR: It is demonstrated that residues 1850-2136 comprising repeats 17-19 contain the binding site for GP Ib-IX, which is a component of the submembranous cytoskeleton and interacts with the glycoprotein (GP) Ibα subunit of the GP Ib -IX complex in platelets.
88 citations
Authors
Showing all 1568 results
| Name | H-index | Papers | Citations |
|---|---|---|---|
| Frank B. Hu | 250 | 1675 | 253464 |
| Bruce M. Psaty | 181 | 1205 | 138244 |
| Bruce N. Ames | 158 | 506 | 129010 |
| Rino Rappuoli | 132 | 816 | 64660 |
| Robert S. Schwartz | 130 | 923 | 62624 |
| Carlos López-Otín | 126 | 494 | 83933 |
| Ronald M. Krauss | 120 | 438 | 77969 |
| Robert S. Stern | 120 | 761 | 62834 |
| Joan S. Brugge | 115 | 286 | 47965 |
| Ewan Birney | 114 | 308 | 125382 |
| Keith M. Sullivan | 105 | 447 | 39067 |
| Bo Lönnerdal | 99 | 674 | 36297 |
| Dennis E. Discher | 98 | 372 | 60060 |
| Richard Reinhardt | 94 | 370 | 58076 |
| Henry A. Erlich | 93 | 354 | 40295 |