Showing papers by "French Institute of Health and Medical Research published in 1970"
TL;DR: α-amanitin selectively inhibits RNA synthesis catalyzed by calf thymus RNA polymerase activity B by interacting with the enzyme and inhibiting chain elongation as discussed by the authors, which can be used to identify the source of RNA synthesis.
449 citations
TL;DR: The premature at 40 weeks of gestational age has not attained a level of organization as high as that of the full-term newborn, and an hypothesis suggesting a possible mechanism of this discrepancy is presented.
Abstract: The ontogenesis of sleep was studied in prematures from 32 to 41 weeks of gestational age The characteristics of the EEG, the patterns and rate of respiration, the cardiac rate, body motility comprised of localized and diffuse movements, localized and diffuse jerks, clonic movements and electromyo-graphic activity of the chin, as well as periods of total quietness were observed and correlated with the progressive organization of behavior in these infants. The data obtained at each gestational age, i.e., at 32–34 weeks, 35–37 weeks, and 38–41 weeks, were compared to those obtained in full-term newborns. The relationships between the different events and the features of the EEG and of the respiratory patterns were ascertained and compared to similar data obtained in the full-term newborn. This comparison shows that the premature at 40 weeks of gestational age has not attained a level of organization as high as that of the full-term newborn. An hypothesis suggesting a possible mechanism of this discrepancy is presented.
199 citations
TL;DR: After injection of 3 H-progesterone to estradiol primed castrated guinea pigs, a radioactive 6.7 S peak was found in the uterus cytosol by sucrose gradient ultracentrifugation in tris 10 mM and the receptor was different from the guinea pig ProgesterOne Binding Plasma Protein.
157 citations
TL;DR: Heteroimmunization of rabbits with saline or perchloric extracts of colonic cancers permits the detection of two cancer‐related antigens, but their concentration is much weaker than in intestinal tumours.
Abstract: Heteroimmunization of rabbits with saline or perchloric extracts of colonic cancers permits the detection of two cancer-related antigens. These antigens may be demonstrated, by direct precipitin reaction, in saline extracts of colonic or rectal adenocarcinomata, of some gastric cancers, and of foetal gut, but not in saline extracts of the non-cancerous adult digestive system. However, precipitin-inhibition reaction shows these antigens in perchloric extracts of non-cancerous colonic mucosa but their concentration is much weaker than in intestinal tumours. Detection of a small amount of these antigens in non-cancerous colonic mucosa implies that they are cancer-related, but not cancer-specific. Identity of one of the antigens with Gold's carcinoembryonic antigen is discussed.
MISE EN EVIDENCE D'ANTIGENES LIES AU CANCER COLIQUE DANS L'APPAREIL DIGESTIF HUMAIN
Deux antigenes lies aux cancers coliques humains ont ete mis en evidence par immunisation de lapins avec des extraits salins ou perchloriques de ces tumeurs. La reaction de precipitation directe en gel d'agarose permet de deceler ces antigenes dans les extraits salins de tumeurs coliques, de certains cancers gastriques et de tube digestif embryonnaire, mais pas dans les extraits salins de muqueuse digestive adulte non cancereuse. La reaction d'inhibition de la precipitation montre cependant que ces antigenes sont presents, a l'etat de traces, dans les extraits perchloriques d'intestin adulte non cancereux. Ces antigenes ne sont donc pas specifiques des cancers coliques; ils sont cependant en relation avec le cancer puisque leur concentration est beaucoup plus elevee dans les tumeurs que dans le tissue normal. Les auteurs etudient l'identite de l'un de ces antigenes et de l'antigene carcino-embryonnaire de Gold.
144 citations
TL;DR: Heart failure was correlated with the extent of cellular alterations in the early stages, but poorly in the later ones, while the average sarcomere length never differed from controls.
76 citations
TL;DR: A method for the quantitative estimation of thyroglobulin iodoamino acid residues is described and definitely improves procedures that are already known.
65 citations
TL;DR: In this article, auteurs rapportent 2 cases of DNAI and discuss the aspects morphologiques, the nature glycolipoproteique and the repartition topographique des spheroides, electivement disposes dans les structures medullaires.
Abstract: Les auteurs rapportent 2 cas de DNAI.Le tableau clinique, d'evolution mortelle en 2 ans, associe un arret du developpement psycho-moteur, des signes pyramidaux et extrapyramidaux, un nystagmus pendulaire avec atrophie optique. L'etude en microscopie optique definit:—les aspects morphologiques, la nature glycolipoproteique et la repartition topographique des spheroides, electivement disposes dans les structures medullaires, bulbo-protuberantielles et cerebelleuses, phylogenetiquement les plus anciennes, —l'atrophie du cortex cerebelleux, —la surcharge soudanophile avec etat dysmyelinique du pallidum, —la degenerescence des voies afferentes et efferentes des fibres longues de la moelle, du tronc cerebral et des voies optiques.L'etude en microscopie electronique precise la structure des spheroides composes d'amas membrano-tubulaires et cisternaux, des corps denses amorphes ou lamelles, multigranuleux, pseudo-cristallins ou multivesiculaires et de mitochondries pathologiques. La formation de ce materiel est liee a l'hyperplasie du reticulum endoplasmique lisse et a l'accumulation de mitochondries anormales dans les pericaryons neuronaux, les prolongements dendro-axonaux, les culs de sacs synaptiques. Les auteurs rapprochent leurs constatations de faits semblables observes en pathologie humaine et experimentale.
48 citations
TL;DR: Calf thymus RNA polymerases A and B are unable to recognize most of the initiation signals which are present in native T 4 -DNA, and the basis for this specificity lies in the native double-stranded structure of DNA.
41 citations
37 citations
25 citations
TL;DR: It is concluded that the presence of nondissociable molecules formed of disulfide-associated subunits can probably be correlated with thyroglobulin iodination in vivo.
TL;DR: The data presented here are consistent with the hypothesis that the embryonic stem cells can be transformed into adult stem cells in the liver of 15 to 16 days old fetuses.
Abstract: The variations in the distribution of hemopoietic stem cells in fetal tissues are concomitant with changes in the electrophoretic pattern of hemoglobin. The data presented here are consistent with the hypothesis that the embryonic stem cells can be transformed into adult stem cells in the liver of 15 to 16 days old fetuses.
TL;DR: Free α- and β-globins and haemoglobin biosynthesis: evidence has been given that, in the cell-free system, the assembly of free α and β chains occurs and the combination of freeα chains with β chains still attached to the ribosomes is possible.
01 Jun 1970
TL;DR: The reaction of older rats brings to evidence a permanent inhibitory mechanism that results in a less regular and intensive response that seems to be correlated with the production of binucleated and polyploÏd hepatocytes.
Abstract: The injection of irritating substances of various chemical nature disturbs the mechanisms regulating the multiplication of rat hepatocytes.The reaction of baby rats is intense and reproducible. It results, 15 hours after the injection, in an increase of the number of cells entering the S phase, so that practically all the divisions which would normally have been scattered over 36 hours are initiated within 2 hours. This burst of synchronized mitosis corresponding to a 36 hours advance in the hepatic growth is followed by a decrease of mitoses due to a feed-back inhibition.The reaction of older rats brings to evidence a permanent inhibitory mechanism that results in a less regular and intensive response.The production of binucleated and polyploId hepatocytes seems to be correlated to this inhibitory mechanism.The regularity and sensivity of the baby rat reactions make them a choice material for the study of hepatic growth.
01 Jun 1970
TL;DR: The injection of irritating substances of various chemical nature disturbs the mechanisms regulating the multiplication of rat hepatocytes, resulting in an increase of the number of cells entering the S phase, so that practically all the divisions which would normally have been scattered over 36 hours are initiated within 2 hours.
Abstract: L'injection de substances irritantes de nature chimique variee perturbe les mecanismes qui contrOlent la multiplication des hepatocytes du rat. Les tres jeunes rats reagissent de faCon intense et reproductible. 15 heures apres l'injection il se produit une augmentation breve et intense du nombre des cellules entrant en phase S de sorte que les divisions cellulaires, qui normalement auraient ete reparties sur les 30 ou 40 prochaines heures, debutent en l'espace de 2 heures seulement. La croissance hepatique a pris une importante avance qui est suivie par une periode de diminution du nombre des mitoses due a une retroaction inhibitrice. La reaction des rats plus âges met en evidence un mecanisme inhibiteur permanent qui entraIne une reponse moins intense et moins reguliere. La production d'hepatocytes binuclees et polyploIdes parait liee a ce mecanisme inhibiteur. La regularite et la sensibilite des reactions des tres jeunes rats en fait un materiel de choix pour l'etude des mecanismes regulateurs de la croissance hepatique.
TL;DR: Evaluation of the various parameters of Laser irradiation resulted in optimal irradiation times for measurement of local stain concentration, as well as for the selective destruction of the stained mitochondria.
Abstract: — Laser micro-irradiation was used to study the uptake of low levels of strain (Janus Green B) by the mitochondria of tissue culture cells and the selective lesions induced by the irradiation. A comparison of the thermal effects produced in the cells and in a stained model system (albumin), permitted the calculation of the degree of concentration of stain in the mitochondria from the staining media (approximately 500 fold). Evaluation of the various parameters of Laser irradiation, particularly thermal events, resulted in optimal irradiation times for measurement of local stain concentration, as well as for the selective destruction of the stained mitochondria. For the studies of the latter phenomenon a lower limit for irradiation duration is set by optical saturation and dielectric breakdown.
TL;DR: This work has suggested the existence of abnormal alleles leading to an impaired synthesis of the heavy chains of one or several immunoglobulins in agammaglobulinaemia.
Abstract: SEVERAL research units have studied the genetic mechanisms controlling the synthesis of immunoglobulins and its alteration in hypogammaglobulinaemia. Fudenberg et al.1 investigated Gm and Inv groups in families with agammaglobulinaemia, and suggested the involvement of two genes in the synthesis of an immunoglobulin: one controlling the synthesis of the heavy chains, the other that of the light chains. Burtin et al.2 applied the same principle in their studies on “non sex-linked atypic hypogammaglobulinaemia”, and postulated the existence of abnormal alleles leading to an impaired synthesis of the heavy chains of one or several immunoglobulins.
TL;DR: The biochemical theories of schizophrenia based on the presence of endogenous psychotogenic agents relate to the hypothesis first advanced by Osmond and Smythies of an aberrant catecholamine metabolism and take into account the studies on experimental animals and humans which demonstrated the psychotomimetic properties of aminochromes.
Abstract: THE biochemical theories of schizophrenia based on the presence of endogenous psychotogenic agents1 relate to the hypothesis first advanced by Osmond and Smythies2 of an aberrant catecholamine metabolism and take into account the studies on experimental animals and humans3 which demonstrated the psychotomimetic properties of aminochromes.
TL;DR: Patients with chronic bronchitis and/or pulmonary emphysema with borderline pulmonary artery Eressure and arterial blood gases have been submitted to aemodynamic studies in order to compare changes in the pulmonary circulation brought about by leg exercise and by voluntary hyperventilation with comparable breathing rate, tidal volume and arterIAL blood gases during both periods.
Abstract: (1) Twelve patients with chronic bronchitis and/or pulmonary emphysema with borderline pulmonary artery Eressure and arterial blood gases have been submitted to aemodynamic studies in order to compare changes in the pulmonary circulation brought about by leg exercise and by voluntary hyperventilation with comparable breathing rate, tidal volume and arterial blood gases during both periods.
(2) Minute ventilation (Exercise: 12.1 ± 1.9 l. min-l m-2; hyperventilation: 12.8± 1.8 1 · min-1· m-2), breathing rate (E: 22.6 ±3.4, H: 23.5 ±3.8), percentage arterial oxygen saturation (E: 94.7±3.3%, H: 95.9±2.7%) and carbon dioxide tension (E: 45.5 ± 5.1 mm Hg, H: 46.8 ± 5.1 mm Hg) did not differ significantly during exercise and hyperventilation, thus suggesting that both mechanical forces acting upon intrathoracic structures and pulmonary vasomotor tone were comparable.
(3) During exercise pulmonary arterial mean pressure (31.4±5.7mm Hg) and pulmonary wedge pressure (13.2± 4.2 mm Hg) rose significantly (p < 0.001) and consistently. During hyperventilation pulmonary arterial mean pressure rose slightly (22.1 ± 5.5 mm Hg, p < 0.05) and the increase in average pulmonary wedge pressure was insignificant (10.0±5.4 mm Hg), although the latter rose markedly in five cases. Pulmonary vascular resistances were not consistently modified. Pulmonary wedge pressure was not correlated with minute ventilation but was positively correlated with the amplitude of respiratory variations in intrathoracic pressure.
(4) Thus, our data suggested that the rise in pulmonary artery and wedge pressures during exercise was due to the combined effects of large intrathoracic pressure swings and of an increase in the haemodynamic load of the left ventricle.
TL;DR: This active dithio ester can be used for degrading peptides, stepwise from their amino end, under mild conditions.
TL;DR: An immunological study of glucose-6-phosphate dehydrogenase in five human deficient mutants, in experimental inactivation with N-ethyl maleimide (NEM) and in the course of red cell ageing shows a decrease of the specific activity of the molecule.
TL;DR: The isolation of this intermediate was identified as an oxazolinone deriving from the intramolecular reaction of the specific substrates, in agreement with the influence of the oxAZolinone on the hydrolysis of the parent Naacyl amino acid amide when utilized as specific substrate for a-chymotrypsin.
TL;DR: In this paper, a gas-liquid chromatography with programmed temperature has been worked out, which allows the simultaneous quantitative measurement of fatty acids, cholesterol and bile acids, which is suitable for the study of the biliary secretion without interrupting the enterohepatic cycle.
TL;DR: All of six patients with NADH diaphorase deficiency studied have been correctly identified using this technique, but it is not suitable for the detection of heterozygotes.
TL;DR: In this paper, the authors present a summary of information on the information on T h e i n t e r f e r o n r e g u r e r e c e l l l i s mediated by I n t E r f ing e r r o r o e r e l l l e l s mediated through a hypothe t ica l a n t i v i r a l a l p r o t e l p R o l e l p
Abstract: The known subs tances involved i n t h e r e g u l a t i o n of t h e c e l l u l a r i n t e r f e r o n mechanism can be d iv ided i n t o t w o ca t egor i e s : r ep res s ion of i n t e r f e r o n syn thes i s i n t h e c e l l , and those involved i n t h e r ep res s ion of t h e a n t i v i r a l s t a t e a f t e r i ts es tab l i shment i n t h e cel l . The derepress ion of t h e a n t i v i r a l s t a t e i n t h e c e l l i s mediated by i n t e r f e r o n i t s e l f through a hypothe t ica l a n t i v i r a l p r o t e i n which probably a c t s on v i r a l p r o t e i n syn thes i s a t t h e ribosomal l e v e l . This r e p o r t is an a t tempt t o summarize p re sen t information on t h e i n t e r f e r o n r egu la to ry mechanism and t o coord ina te a v a i l a b l e d a t a . I n add i t ion , a genera l o u t l i n e of t h i s r egu la to ry mechanism i s t e n t a t i v e l y proposed. those concerned wi th t h e r ep res s ion and de-
TL;DR: The binding between RNA and histone does not occur in the presence of urea and NaCl, and the association is able to combine with 100 μg of RNA.
TL;DR: In this article, the presence d'aflatoxine B was investigated in 380 echantillons d'aliments composes destines au betail et de matieres premieres servant a la fabrication of ces aliments, and 167 prelevements examines contenaient de laflatoxin.
TL;DR: Inhibition of testosterone oxidation by androsta-4-ene-3,17-dione and 17-oxosteroid conjugates suggests that “adrenal androgens” may play a regulatory role on the male hormone inactivating oxidation.
TL;DR: Une methode automatique de depistage et l'identification d'allo-anticorps anti-erythrocytaires utilisant l'Auto-Analyzer emploie un principe d'agglutination artificielle non specifique permetant de potentialiser l'agGLutination due a la presence d'un anticorps.
Abstract: Resume Les auteurs decrivent une methode automatique de depistage et d'identification d'allo-anticorps anti-erythrocytaires utilisant l'Auto-Analyzer. Cette methode emploie un principe d'agglutination artificielle non specifique permetant de potentialiser l'agglutination due a la presence d'un anticorps. Elle permet le depistage et l'identification de la majorite des allo-anticorps diriges contre des antigenes de groupes sanguins, a l'exception de certains anticorps naturels de type γM.