Showing papers by "German Criminal Police Office published in 2015"

RNA/DNA co-analysis from human skin and contact traces - Results of a sixth collaborative EDNAP exercise

Abstract: The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. Eight stains, a skin RNA dilution series and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 22 participating laboratories using RNA extraction or RNA/DNA co-extraction methods. Two sets of previously described skin-specific markers were used: skin1 pentaplex (LCE1C, LCE1D, LCE2D, IL1F7 and CCL27) and skin2 triplex (LOR, KRT9 and CDSN) in conjunction with a housekeeping gene, HKG, triplex (B2M, UBC and UCE). The laboratories used different chemistries and instrumentation. All laboratories were able to successfully isolate and detect mRNA in contact traces (e.g., human skin, palm-, hand- and fingerprints, clothing, car interiors, computer accessories and electronic devices). The simultaneous extraction of RNA and DNA provides an opportunity for positive identification of the tissue source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling. The skin markers LCE1C and LOR and the housekeeping gene marker B2M were detected in the majority of contact traces. Detection of the other markers was inconsistent, possibly due to the low amounts and/or poor quality of the genetic material present in shed skin cells. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid/tissue identification method that can easily be combined with current STR typing technology.

Global spatial distributions of nitrogen and carbon stable isotope ratios of modern human hair

Abstract: RATIONALE Natural stable carbon (δ(13)C) and nitrogen isotope ratios (δ(15)N) of humans are related to individual dietary habits and environmental and physiological factors. In forensic science the stable isotope ratios of human remains such as hair and nail are used for geographical allocation. Thus, knowledge of the global spatial distribution of human δ(13)C and δ(15)N values is an essential component in the interpretation of stable isotope analytical results. METHODS No substantial global datasets of human stable isotope ratios are currently available, although the amount of available (published) data has increased within recent years. We have herein summarised the published data on human global δ(13)C andδ(15)N values (around 3600 samples) and added experimental values of more than 400 additional worldwide human hair and nail samples. In order to summarise isotope ratios for hair and nail samples correction factors were determined. RESULTS The current available dataset of human stable isotope ratios is biased towards Europe and North America with only limited data for countries in Africa, Central and South America and Southeast Asia. The global spatial distribution of carbon isotopes is related to latitude and supports the fact that human δ(13)C values are dominated by the amount of C4 plants in the diet, either due to direct ingestion as plant food, or by its use as animal feed. In contrast, the global spatial distribution of human δ(15)N values is apparently not exclusively related to the amount of fish or meat ingested, but also to environmental factors that influence agricultural production. CONCLUSIONS There are still a large proportion of countries, especially in Africa, where there are no available data for human carbon and nitrogen isotope ratios. Although the interpretation of modern human carbon isotope ratios at the global scale is quite possible, and correlates with the latitude, the potential influences of extrinsic and/or intrinsic factors on human nitrogen isotope ratios have to be taken into consideration.

Forensic ancestry analysis with two capillary electrophoresis ancestry informative marker (AIM) panels: Results of a collaborative EDNAP exercise

Abstract: There is increasing interest in forensic ancestry tests, which are part of a growing number of DNA analyses that can enhance routine profiling by obtaining additional genetic information about unidentified DNA donors. Nearly all ancestry tests use single nucleotide polymorphisms (SNPs), but these currently rely on SNaPshot single base extension chemistry that can fail to detect mixed DNA. Insertion-deletion polymorphism (Indel) tests have been developed using dye-labeled primers that allow direct capillary electrophoresis detection of PCR products (PCR-to-CE). PCR-to-CE maintains the direct relationship between input DNA and signal strength as each marker is detected with a single dye, so mixed DNA is more reliably detected. We report the results of a collaborative inter-laboratory exercise of 19 participants (15 from the EDNAP European DNA Profiling group) that assessed a 34-plex SNP test using SNaPshot and a 46-plex Indel test using PCR-to-CE. Laboratories were asked to type five samples with different ancestries and detect an additional mixed DNA sample. Statistical inference of ancestry was made by participants using the Snipper online Bayes analysis portal plus an optional PCA module that analyzes the genotype data alongside calculation of Bayes likelihood ratios. Exercise results indicated consistent genotyping performance from both tests, reaching a particularly high level of reliability for the Indel test. SNP genotyping gave 93.5% concordance (compared to the organizing laboratory's data) that rose to 97.3% excluding one laboratory with a large number of miscalled genotypes. Indel genotyping gave a higher concordance rate of 99.8% and a reduced no-call rate compared to SNP analysis. All participants detected the mixture from their Indel peak height data and successfully assigned the correct ancestry to the other samples using Snipper, with the exception of one laboratory with SNP miscalls that incorrectly assigned ancestry of two samples and did not obtain informative likelihood ratios for a third. Therefore, successful ancestry assignments were achieved by participants in 92 of 95 Snipper analyses. This exercise demonstrates that ancestry inference tests based on binary marker sets can be readily adopted by laboratories that already have well-established CE regimes in place. The Indel test proved to be easy to use and allowed all exercise participants to detect the DNA mixture as well as achieving complete and concordant profiles in nearly all cases. Lastly, two participants successfully ran parallel next-generation sequencing analyses (each using different systems) and achieved high levels of genotyping concordance using the exercise PCR primer mixes unmodified.

Ink dating using thermal desorption and gas chromatography/mass spectrometry: comparison of results obtained in two laboratories.

Abstract: An ink dating method based on solvent analysis was recently developed using thermal desorption followed by gas chromatography/mass spectrometry (GC/MS) and is currently implemented in several forensic laboratories. The main aims of this work were to implement this method in a new laboratory to evaluate whether results were comparable at three levels: (i) validation criteria, (ii) aging curves, and (iii) results interpretation. While the results were indeed comparable in terms of validation, the method proved to be very sensitive to maintenances. Moreover, the aging curves were influenced by ink composition, as well as storage conditions (particularly when the samples were not stored in “normal” room conditions). Finally, as current interpretation models showed limitations, an alternative model based on slope calculation was proposed. However, in the future, a probabilistic approach may represent a better solution to deal with ink sample inhomogeneity.

Trust in the German Police: Determinants and Consequences for Reporting Behavior

Abstract: According to Tyler’s procedural justice theory, public trust in or perceived legitimacy of the police plays a central role in enhancing citizens’ cooperation with this institution. Reporting a crime to the police marks one important form of public cooperation. This chapter examines the interrelationships between personal experiences with the police, various dimensions of trust in the police, and victims’ reporting behaviors, based on a large-scale victimization survey from Germany. Results provide partial support for the hypothesized relationships, but also reveal dangerous pitfalls when approaching this issue with data collected in standard victimization surveys.

Assessing mode effects in online and telephone victimization surveys

Abstract: Victimization surveys not only represent a vital data source for estimating the amount of crime within a social entity but also provide victimological researchers with valuable information, e.g. about the social distribution and consequences of victimization experiences. To assure adequate interpretation of victimization survey data, knowledge about the data-generating process in general and the measurement of victimization experiences in particular is essential. In 2010, a new round of the International Crime and Victim Survey (ICVS-2) was conducted to explore the different effects of telephone and online victimization surveys. To understand the processes behind these, this study follows a theory-driven approach. Based on a four-step cognitive model of survey response, hypotheses about expected mode effects on the response behavior were developed. For hypothesis testing, a hierarchical modeling strategy based on logistic regressions was applied to the ICVS-2 data. As hypothesized, no effects of survey mo...

Finding the AES Bits in the Haystack: Reverse Engineering and SCA Using Voltage Contrast

Abstract: In this paper, we demonstrate how the Scanning Electron Microscope (SEM) becomes a powerful tool for Side Channel Analysis (SCA) and Hardware Reverse Engineering. We locate the AES hardware circuit of a XMEGA microprocessor with Capacitive-Coupled Voltage Contrast (CCVC) images and use them in a powerful Voltage Contrast Side Channel Analysis (VCSCA). This enables an attacker to locate AES bit-wires in the top metal-layer and thus, to recover valuable netlist information. An attacker gets a valuable entry-point to look for weaknesses or Intellectual Property (IP) in the AES circuit. Additionally we show the great potential of the VCSCA in a non-invasive Side Channel Analysis for Reverse Engineering (SCARE) approach. Finally, we recover the full key of the AES hardware-engine in a practical template-based VCSCA and a no-plaintext, no-ciphertext and no-key Simple Side Channel Analysis (SSCA). We show that future VCSCA attacks present a big hardware security-risk that IC vendors need to consider.

Nonresponse Bias in telefonischen Opferbefragungen

Abstract: Die national sowie international niedrigen und sinkenden Ausschopfungsquoten in Umfragen (De Leeuw und De Heer 2002; Aust und Schroder 2009) konnen als eines der Hauptprobleme der heutigen Umfrageforschung betrachtet werden. Auch wenn niedrige Ausschopfungsquoten nicht zwingend mit Verzerrungseffekten einhergehen (Groves und Peytcheva 2008; Schouten et al. 2009), so ist die Reprasentativitat von Umfrageergebnissen bei hohen Nonresponseraten jedoch zumindest in Frage zu stellen – auch wenn einige Studien feststellen konnten, dass hohere Ausschopfungen ebenso mit schlechteren Schatzern einhergehen konnen (Groves 1989; Groves und Couper 1998).