Institution
Helmholtz Centre for Environmental Research - UFZ
Facility•Leipzig, Germany•
About: Helmholtz Centre for Environmental Research - UFZ is a facility organization based out in Leipzig, Germany. It is known for research contribution in the topics: Population & Species richness. The organization has 3230 authors who have published 9880 publications receiving 394385 citations.
Papers published on a yearly basis
Papers
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TL;DR: The observed decrease of the diclofenac concentration in waters and the formation of its photochemical transformation products under the impact of natural irradiation during one to 16 days are explained in this article.
110 citations
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TL;DR: This paper provides a synthesis of available approaches, models and tools, and data sources, that are able to better link ecosystem service mapping to current understanding of the role of ecosystem service providing organisms and land/seascape structure in ecosystem functioning and opens avenues for further model development using hybrid approaches tailored to available resources.
110 citations
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TL;DR: In this article, the authors explored how and which ecosystem services are currently considered in the SDGs and the Aichi targets and identified which information might be already available for monitoring progress towards their goals by reviewing national ecosystem assessments.
109 citations
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TL;DR: The contribution of PAH intake via oyster consumption to carcinogenic risks in humans seems to be low, and no correlation between sediment and oyster concentrations at the same sites was found.
109 citations
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TL;DR: Direct molecular evidence is presented that the average PCN does not represent the true number of plasmid molecules in individual cells and is generally at the lower end of previously reported ranges.
Abstract: Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms of average plasmid copy number (PCN) and stability are rarely known. The crucial factor determining the PCN is the replication system; most replication systems in use today belong to a small number of different classes and are available through repositories like the Standard European Vector Architecture (SEVA). In this study, the PCN was determined in a set of seven SEVA-based expression plasmids only differing in the replication system. The average PCN for all constructs was determined by Droplet Digital PCR and ranged between 2 and 40 per chromosome in the host organism Escherichia coli. Furthermore, a plasmid-encoded EGFP reporter protein served as a means to assess variability in reporter gene expression on the single cell level. Only cells with one type of plasmid (RSF1010 replication system) showed a high degree of heterogeneity with a clear bimodal distribution of EGFP intensity while the others showed a normal distribution. The heterogeneous RSF1010-carrying cell population and one normally distributed population (ColE1 replication system) were further analyzed by sorting cells of sub-populations selected according to EGFP intensity. For both plasmids, low and highly fluorescent sub-populations showed a remarkable difference in PCN, ranging from 9.2 to 123.4 for ColE1 and from 0.5 to 11.8 for RSF1010, respectively. The average PCN determined here for a set of standardized plasmids was generally at the lower end of previously reported ranges and not related to the degree of heterogeneity. Further characterization of a heterogeneous and a homogeneous population demonstrated considerable differences in the PCN of sub-populations. We therefore present direct molecular evidence that the average PCN does not represent the true number of plasmid molecules in individual cells.
109 citations
Authors
Showing all 3363 results
Name | H-index | Papers | Citations |
---|---|---|---|
Debbie A Lawlor | 147 | 1114 | 101123 |
Sandra Lavorel | 101 | 321 | 58963 |
Stephen P. Hubbell | 101 | 249 | 41904 |
Henri Weimerskirch | 100 | 413 | 29338 |
Alfons J. M. Stams | 93 | 464 | 30395 |
Andrew K. Skidmore | 84 | 529 | 29944 |
Richard Condit | 82 | 228 | 26685 |
Wolfgang W. Weisser | 80 | 392 | 22569 |
Ingolf Kühn | 76 | 222 | 25573 |
Beate I. Escher | 74 | 294 | 18425 |
Jörg Kärger | 73 | 604 | 20918 |
Dagmar Haase | 72 | 276 | 15961 |
Josef Settele | 68 | 295 | 24919 |
Nico Eisenhauer | 66 | 400 | 15746 |
Josef Cyrys | 65 | 214 | 15064 |