Institution
Mount Desert Island Biological Laboratory
Nonprofit•Bar Harbor, Maine, United States•
About: Mount Desert Island Biological Laboratory is a nonprofit organization based out in Bar Harbor, Maine, United States. It is known for research contribution in the topics: Squalus acanthias & Spiny dogfish. The organization has 898 authors who have published 819 publications receiving 34283 citations.
Topics: Squalus acanthias, Spiny dogfish, Skate, Sodium, Gene
Papers published on a yearly basis
Papers
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TL;DR: It is demonstrated that Bsep is a liver-specific ATP-dependent export pump that is highly conserved throughout evolution and insights into critical determinants for the function of this transporter in higher vertebrates are provided.
Abstract: Bile secretion is a fundamental function of the liver of all vertebrates and is generated by ATP-dependent transport proteins at the canalicular membrane of hepatocytes, particularly by the bile salt export pump BSEP. To determine the evolutionary origin and structure-function relationship of this transport mechanism, a liver cDNA library from the marine skate Raja erinacea, a 200 million-year-old vertebrate, was screened for BSEP orthologues. A full-length clone was isolated that encodes for 1,348 amino acids and shares 68.5% identity to human BSEP. Northern blot analysis revealed a 5-kb transcript only in skate liver. Expression of skate Bsep in Sf9 cells demonstrated a sixfold stimulation of ATP-dependent taurocholate transport compared with controls, with a Michaelis-Menten constant of 15 μM, which is comparable to rat Bsep. Sequences at the site of published mutations in human BSEP are also conserved in skate Bsep. When two of these mutations were introduced into the skate Bsep cDNA, this resulted in...
60 citations
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TL;DR: This overview emphasizes some of the special attributes of alternative animal models that may be exploited to define the molecular and cellular basis of toxicity and highlights the use of fish, avian, Drosophila, Caenorhabditis elegans, and yeast models in such studies.
59 citations
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TL;DR: Experimental observations suggest the existence of a transport system for divalent cations separate from another fordivalent anions within each transport system, where the ion with the higher excretion rate depressed competitively transfer of the other ion.
Abstract: A characterization was attempted of the mechanisms involved in the tubular transport of inorganic divalent ions by the aglomerular kidney of Lophius, attention being paid particularly to the possible existence of transport maxima (Tm) and to competition for transport among related substances undergoing tubular excretion. Excretory rates of divalent ions in non-treated fish during standard laboratory conditions paralleled spontaneous changes in urine flow. Tm rates of excretion were reached for magnesium, sulfate, and thiosulfate with corresponding plasma levels of 2 to 5, 5 to 17, and 4 to 12 µM/ml. respectively. Elevation of magnesium chloride levels in plasma markedly depressed calcium excretion; sodium thiosulfate similarly depressed sulfate excretion. Experimental observations suggest the existence of a transport system for divalent cations separate from another for divalent anions. Within each transport system the ion with the higher excretion rate depressed competitively transfer of the other ion. Neither system was influenced by probenecid (benemid) in doses which markedly depressed the simultaneous excretion rate of p-aminohippuric acid.
59 citations
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TL;DR: A new extrinsic input, the post-oesophageal commissure (POC) neurons, are identified, relatively brief stimulation of which triggers a long-lasting activation of this novel gastric mill rhythm at least in part via its lasting activation of MCN1 and CPN2.
Abstract: Neuronal network flexibility enables animals to respond appropriately to changes in their internal and external states. We are using the isolated crab stomatogastric nervous system to determine how extrinsic inputs contribute to network flexibility. The stomatogastric system includes the well-characterized gastric mill (chewing) and pyloric (filtering of chewed food) motor circuits in the stomatogastric ganglion. Projection neurons with somata in the commissural ganglia (CoGs) regulate these rhythms. Previous work characterized a unique gastric mill rhythm that occurred spontaneously in some preparations, but whose origin remained undetermined. This rhythm includes a distinct protractor phase activity pattern, during which a key gastric mill circuit neuron (LG neuron) and the projection neurons MCN1 and CPN2 fire in a pyloric rhythm-timed activity pattern instead of the tonic firing pattern exhibited by these neurons during previously studied gastric mill rhythms. Here we identify a new extrinsic input, the post-oesophageal commissure (POC) neurons, relatively brief stimulation (30 s) of which triggers a long-lasting (tens of minutes) activation of this novel gastric mill rhythm at least in part via its lasting activation of MCN1 and CPN2. Immunocytochemical and electrophysiological data suggest that the POC neurons excite MCN1 and CPN2 by release of the neuropeptide Cancer borealis tachykinin-related peptide Ia (CabTRP Ia). These data further suggest that the CoG arborization of the POC neurons comprises the previously identified anterior commissural organ (ACO), a CabTRP Ia-containing neurohemal organ. This endocrine organ thus appears to also have paracrine actions, including activation of a novel and lasting gastric mill rhythm.
58 citations
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TL;DR: The binding of [3H]benzmetanide to secretory tubules isolated from dogfish shark rectal glands suggested that separate signal transduction pathways, one sensitive to cAMP and another to cell volume, regulate the Na-K-Cl cotransporter.
Abstract: We examined the binding of [3H]benzmetanide, a potent inhibitor of Na-K-Cl cotransport, to secretory tubules isolated from dogfish shark rectal glands. Specific binding increased dramatically (from...
58 citations
Authors
Showing all 901 results
Name | H-index | Papers | Citations |
---|---|---|---|
David C. Page | 110 | 509 | 44119 |
Hermann Haller | 98 | 706 | 37167 |
Jonathan A. Epstein | 94 | 299 | 27492 |
James L. Boyer | 90 | 432 | 25432 |
David H. Evans | 89 | 430 | 28093 |
Rui Wang | 72 | 228 | 25599 |
Carroll E. Cross | 70 | 273 | 21037 |
George R. Dubyak | 68 | 203 | 16498 |
Charles Nicholson | 68 | 155 | 18804 |
Shuk-Mei Ho | 67 | 248 | 17410 |
Billy G. Hudson | 67 | 268 | 15353 |
Franklin H. Epstein | 67 | 217 | 15510 |
Fuad N. Ziyadeh | 66 | 159 | 17127 |
John R. Riordan | 65 | 137 | 17394 |
Ronald M. Lechan | 65 | 218 | 13317 |