Showing papers by "Osaka University published in 1970"
610 citations
TL;DR: In this paper, eight different substances were isolated in crystalline forms by ion exchange chromatography of the aliphatic basic amino acid fraction of human urine and the identities of the compounds were confirmed by synthesis followed by comparison of infrared and nuclear magnetic resonance spectra and of chromatographic properties.
384 citations
TL;DR: An apparatus with which the continuous oxygen equilibrium curve of hemo globin can be recorded automatically was constructed and its performance was examined, finding it suitable for studies on the function of abnormal hemoglobins.
333 citations
TL;DR: In this paper, the crystal structure of poly-e-caprolactone was determined by interpretation of X-ray diffraction patterns, showing that the CH2 sequences are planar but the plane of atoms of the ester group tilts slightly to the fiber axis.
Abstract: In the course of structural studies of aliphatic polyesters, the crystal structure of poly-e-caprolactone, [–(CH2)5–CO–]n, was determined by interpretation of X-ray diffraction patterns. The crystallographic data are: a=7.47A, b=4.98A, c(fiber axis) =17.05A, the orthorhombic space group P212121-D24, two molecular chains in the unit cell. The chain conformation of poly-e-caprolactone is almost planar zigzag, but evidently deviates from the fully extended form, i.e., the CH2 sequences are planar but the plane of atoms of the ester group tilts slightly to the fiber axis. The molecular chains are arranged side by side in the same way as in polyethylene, but the carbonyl groups of the two chains in the unit cell are separated by an amount of 3/14c along the fiber axis.
254 citations
173 citations
TL;DR: In this article, a neutron diffraction study has been made on single crystal specimens of α-Mn at 4.4°K, 60°K and several other temperatures.
Abstract: A neutron diffraction study has been made on single crystal specimens of α-Mn at 4.4°K, 60°K, and several other temperatures. Five types of magnetic form factor were in turn employed in the analysis. The magnetic structure is described by a single non-collinear configuration throughout the temperature range below T N with relatively large magnetic moments in Sites I, II and small moments in Sites III, IV, irrespective of which form factor is chosen. The experimental results seem to favor a localized moment model rather than a spin density wave model. When the analysis is based on the localized moment model, moments of 1.9, 1.7, 0.6 and 0.2 5 µ B may be assigned to Site I, II, III, and IV, respectively at 4.4°K, and the spin density in the atom is a superposition of a positive 3d-density and a negative 4s-density. The dependence of the effective exchange interaction coefficient on the inter-atomic distance is discussed.
167 citations
142 citations
TL;DR: Isoamylase was purified about 720-fold in 23% yield to a final specific activity of 59 100 per mg protein from the culture fluid of Pseudomonas sp.
129 citations
126 citations
TL;DR: This theorem completely characterizes the codewords of the
u th-order Reed-Muller code whose weights are less than twice the minimum weight and leads to the weight enumerators for thosecodewords.
Abstract: The following theorem is proved. Let f(x_1,\cdots, x_m) be a binary nonzero polynomial of m variables of degree
u . H the number of binary m -tuples (a_1,\cdots, a_m) with f(a_1, \cdots, a_m) = 1 is less than 2^{m-
u+1} , then f can be reduced by an invertible affme transformation of its variables to one of the following forms. \begin{equation} f = y_1 \cdots y_{
u - \mu} (y_{
u-\mu+1} \cdots y_{
u} + y_{
u+1} \cdots y_{
u+\mu}), \end{equation} where m \geq
u+\mu and
u \geq \mu \geq 3 . \begin{equation} f = y_1 \cdots y_{
u-2}(y_{
u-1} y_{
u} + y_{
u+1} y_{
u+2} + \cdots + y_{
u+2\mu -3} y_{
u+2\mu-2}), \end{equation} This theorem completely characterizes the codewords of the
u th-order Reed-Muller code whose weights are less than twice the minimum weight and leads to the weight enumerators for those codewords. These weight formulas are extensions of Berlekamp and Sloane's results.
110 citations
TL;DR: Sequenced polytripeptides, (Pro‐Pro‐Gly)n, with defined molecular weights were synthesized by the solid‐phase method, and each of these polymers existed as a timer at lower temperature, and the others were expected to behave analogously.
Abstract: Sequenced polytripeptides, (Pro-Pro-Gly)n, (n = 10, 15, 20), with defined molecular weights were synthesized by the solid-phase method. Conformational changes of these sample as a function of temperature were studied by measurements of optical rotation and sedimentation equilibrium. The temperature dependence of optical rotation was shown similar to thermal transition of collagen molecule. Each of these polymers existed as a timer at lower temperature. (Pro-Pro-Gly)10 existed as a monomer at higher temperature, and the others were expected to behave analogously.
TL;DR: Electron microscopy showed nexuses in the regions of intercellular contact between myocardial and FL cells and between FL cells.
TL;DR: Effects of EDTA and various proteolytic enzymes on disaggregation of the pseudoplasmodium (slug) of the cellular slime mold Dictyostelium discoideum were studied and pronase plus BAL was most effective and did not require the presence of salt for complete disaggregation.
TL;DR: The locus coeruleus of the rat was studied with the Golgi method and electron microscope in an attempt to demonstrate morphological substrates for the functional significance of these monoamine neurons.
Abstract: The locus coeruleus of the rat was studied with the Golgi method and electron microscope in an attempt to demonstrate morphological substrates for the functional significance of these monoamine neurons.The locus coeruleus is mainly composed of densely packed medium-sized, spindle-shaped nerve cells with frequent soma spines, while in the rostral part of the nucleus the medium-sized cells are less densely located and mingled with small oval cells. Most of the axons originating from the medium-sized nerve cells take a rostro-ventral course usually giving off an initial collateral. It is interesting that some of the axons demonstrate a curious recurrent way or run caudalwards to the medulla oblongata after a short rostro-ventral course. Afferent fibers are composed of thick and fine fibers, which make complicated neuropil around the nurons. The thick afferents enter the nucleus from a ventral to dorsal direction and take an undulating tortuous course among the neurons, revealing axonal swelling and giving off many terminal boutons.The cytoplasm of the medium-sized nerve cells demonstrates marked aggregates of rough surfaced ER and prominent development of Golgi complexes, and contains a large number of lysosomal dense bodies. Large granular vesicles as seen in the terminal bags are only small in number in the cytoplasm. Cytoplasmic protrusions are characteristic for the medium-sized nerve cells, being moderate in number, varying in shape and length (0.4-1.7μ) and constituting mostly the postsynaptic sites. Axo-somatic synapses are frequent which make contact with axon terminals similar to axo-soma spine synapses. The nuropil contains different kinds of axo-dendritic and axo-dendritic spine synapses. Occasionally one can observe thickened preterminal axons or axon terminals contacting several dendrites or dendritic spines. Astroglial cells fill up the interneuronal spaces and demonstrate large oval mitochondria with homogeneous content and occasional lipofuscin granules.
TL;DR: The primary structure of the purified cardiotoxin from Naja naja atra venom, containing 60 amino acid residues, was determined and it was found that 20 residues including 8 half cystines are identical.
TL;DR: In this article, the phase transition of the condensed phase of KMnF 3 has been studied in detail by X-ray scattering and the anomalous scattering that accompanies the transition near T c is found to be anisotropic for q ∼ 0.05A -1.
Abstract: The phase transition that KMnF 3 exhibits at T c =184°K has been studied in detail by X-ray scattering. The anomalous scattering that accompanies the transition near T c is found to be anisotropic for q ∼0.05A -1 . For these wave vectors therefore, the Ornstein-Zernike approximation for the density-density correlation function cannot describe the critical properties. The temperature dependence of the square of the frequency of the soft \(\varGamma_{25}\) mode at R is found to be reasonably well described by a Curie-law divergence. The space group of the condensed phase of KMnF 3 has been reexamined. The space group is found to be the tetragonal I 4/ m c m ( D 4 h 18 ) rather than the orthorhombic P b n m ( D 2 h 16 ) proposed by Beckman and Knox. The space groups of the condensed phases of KMnF 3 and SrTiO 3 are therefore identical.
TL;DR: In this article, a growing-chain-controlled mechanism was proposed for the polymerization of trityl (TMA), diphenylmethyl (DMA), and benzyl (BMA) methacrylates.
Abstract: Polymerizations of trityl (TMA), diphenylmethyl (DMA) and benzyl (BMA) methacrylates were carried out mainly by n-butyllithium (n-BuLi) and the tacticities of the polymers were studied by NMR spectroscopy. Polymerization of TMA by n-BuLi gave a highly isotactic polymer in toluene as well as in tetrahydrofuran (THF). The polymer obtained in radical polymerization by AIBN catalyst had about 60% isotacticity in triads. From these results the growing-chain-controlled mechanism was proposed to this polymerization. In the polymerization of DMA by n-BuLi a highly isotactic polymer was obtained in toluene at lower temperatures and a highly syndiotactic one in THF. The radical polymerization gave a predominantly syndiotactic polymer. Temperature dependence of the NMR signal-width of the isotactic polymer indicates that the conformational arrangement of the polymer chain in solution is much rigid up to a fairly higher temperature. These results suggest that the growing-chain-controlled mechanism may contribute to some extent to the stereoregulation of DMA in toluene. BMA behaved very similarly to methyl methacrylate in the polymerization by n-BuLi. Probably the same polymerization mechanisms can be applied for both monomers. Polymerization of TMA, DMA and BMA by phenylmagnesium bromide or diethylaluminum diphenylamide was also investigated.
TL;DR: It is concluded that res(-) mutants are defective in the second step (repair synthesis) of the excision repair process and that DNA polymerase is partly responsible for the assumed resynthesis step.
Abstract: Alleles responsible for X-ray-sensitive characteristics of three mutants of Escherichia coli B, which were also sensitive to ultraviolet (UV) irradiation, were mapped near metE locus, and named res-1, res-2, and res-3. All the res− mutants showed no host cell reactivability (Hcr−) for transducing deoxyribonucleic acid (DNA) of P1 phage irradiated by UV but they were Hcr+ for infective DNA of P1 phage. Furthermore, they showed no detectable activity of DNA polymerase. Characteristics of allele res-1 were studied in detail. The mutant res-1 uvr+ showed an extensive degradation of DNA after UV irradiation. Double mutants carrying res-1 uvrA−, res-1 uvrB−, and res-1 uvrC− showed no marked increase in UV sensitivity beyond that of the uvr− single mutants and only negligible UV-induced DNA degradation. The uvr− mutations showed no such suppressive effect on DNA degradation induced by X rays in these double mutants. It is concluded that res− mutants are defective in the second step (repair synthesis) of the excision repair process and that DNA polymerase is partly responsible for the assumed resynthesis step.
TL;DR: Maintained and evoked unit activity in the mesencephalic reticular formation (MRF) was studied in behaving cats, and Gradation of unitary responsiveness in the three states was established as light sleep→arousal→deep sleep in order of increasing sensitivity.
TL;DR: In this article, the Grignard reagents were used to obtain benzyl-cobalt complexes of type π-C5H5Co(L)RR' [L = Ph3P, Ph2CH3P and Ph3As;R,R' = CH3, PhCH2 and I] were prepared from the reaction of π -C5h5Co (L)I2 with Grignards reagents.
TL;DR: A new type of natural pyrrhotite, orthorhombic 11C type, and the hexagonal 6C type are described, both of which are considered metastable in nature.
Abstract: A new type of natural pyrrhotite, orthorhombic 11C type (a = 6.892, b = 11.952, c = 5.744 x 11 angstroms), and the hexagonal 6C type (a = 6.89, c = 5.76 x 6 angstroms) are described. Their compositions are Fe(10)S(11) and Fe(11)S(12), respectively. Pyrrhotites stable in nature have essentially stoichiometric composition, Fe(n)-(l)S(n) (n>/=8), with the structures of n/2C type for n even and of nC type for n odd. The solid solutions between Fe(11)S(12) and Fe(10)S(11), and between Fe(10)S(11) and Fe(9)S(10) are considered metastable in nature.
TL;DR: In this article, a perovskite CaFeO 3 with a o = 3.770 A has been synthesized at high temperature and pressure and an isomer shift corresponding to the tetravalent state of iron and a Neel temperature of 120°K were obtained from Mossbauer spectra and magnetic susceptibility measurements.
TL;DR: The present series of reactions affords a convenient and novel method for the synthesis of purine cyclonucleoside through sulfonylation using sodium hydride and triisopropyl-benzenesulfonyl chloride in N,N-dimethylformamide solution.
Journal Article•
TL;DR: In the rabbit, cat, and bovine corneas, the anterior segment was less hydrophilic than the posterior one, in both the central and peripheral regions, while in the rabbit cornea, the swelling ratio of the posterior segment was significantly larger in the central region than in the peripheral.
Abstract: The surface V3 of the denuded corneal stroma was separated into the anterior and posterior segments; the swelling ratio of each was compared under zero pressure. In the rabbit, cat, and bovine corneas, the anterior segment was less hydrophilic than the posterior one, in both the central and peripheral regions. In the rabbit cornea, the swelling ratio of the posterior segment was significantly larger in the central region than in the peripheral. A fine glass capillary was implanted into the stroma as a landmark on the living eye. Corneal swelling was induced by means of irrigation with cold Ringer's solution. In vivo, under normal intraocular pressure, as in vitro, the anterior layers were less hydrophilic than the posterior. In order to clarify the relationship between uneven swelling and clouding of the stroma, successive observations of light scattering and simultaneous thickness measurements were made on the anterior and posterior layers of the enucleated eye at the various stages of swelling. An intense light scattering occurred with slight swelling in the anterior layers, while in the posterior layers, relatively slight scattering occurred with a high degree of swelling. This paradoxical finding leads to the supposition that the hydration of the stroma bears quite a different relation to light scattering in the respective layers. The principle that light scattering occurs in proportion to the degree of swelling cannot be applied to the other zone beyond the boundary between the anterior and posterior zones.
TL;DR: In this paper, a split sphere multipiston assemblage is introduced with which it is possible to produce ultra high pressure and temperature for synthetic experiments and a phase diagram of the system is presented.
Journal Article•
TL;DR: In this article, the synthesis and crystallographic studies of the digenite-t5,pe solid solution indicate that the solid solution is metastable at room temperature and decomposes to mixtures of anilite and djurleite.
Abstract: been studied by the X-ray difiraction and microscopy. At room temperature, the stable phases in the range of composition studied are djurleite (Cur.rtS), anilite (Cu1 rsS) and covellite (CuS). Above 70o+3oc, anilite decomposes to high digenite and covellite. The digenite-type solid solution usually appears in synthetic experiments with composition between Cur.za;S and Cur zgS at room temperature as indicated by Roseboom (1966), but its composition range occasionally extends from Cur.zsS to Cur eoS. The cell dimensions are irrationaliy related to the cubic subcell of high digenite, varying with composition. The synthesis and crystallographic studies of the digenite-t5,pe solid solution indicate that the solid solution is metastable at room temperature and decomposes to mixtures of anilite and djurleite. Natural digenite always contains a small amount of iron and is considered to be stable only in the Cu-Fe-S system.
TL;DR: In this paper, the early stages of growth of martensite platelets were observed by means of electron microscopy, and the authors found that very thin martensites were found to be in association with austenite stacking faults.
TL;DR: In this paper, magnetic properties of one dimensional antiferromagnet Cu(C6HsC00)2 ·3H20 were investigated by means of magnetic susceptibility, magnetic torque, NMR, and ESR measurements.
Abstract: Various magnetic properties of one dimensional antiferromagnet Cu(C6HsC00)2 ·3H20 were investigated by means of magnetic susceptibility, magnetic torque, NMR, and ESR measurements. The results show that this compound is one of the best one dimensional antiferromagnets known at present. An intrachain exchange interaction J/K was estimated to be -8. 6±1 °K while no second order phase change was found down to 1. 4 °K. ESR line width at room temperature was not explained by an usual treatment and an anisotropic exchange effect was newly taken into account. However, other anomalies of the line width such as the frequency, temperature, and angular dependences appeared at low temperature regions were not yet explained.