Polytechnic Institute of Lisbon

About: Polytechnic Institute of Lisbon is a education organization based out in Lisbon, Portugal. It is known for research contribution in the topics: Monetary policy & New Keynesian economics. The organization has 261 authors who have published 368 publications receiving 3569 citations. The organization is also known as: Polytechnical Institute of Lisbon.

Virus bioburden in hospital environment

Abstract: For the past years, major concerns have emerged regarding the prevalence of pathogenic viruses, potentially transmitted by airborne and droplet, in health facilities particularly in the context of immunocompromised patients exposure. The ability of numerous viruses to persist in inanimate environments may endorse viral nosocomial outbreaks on health care workers and patients with significant associated mobility and mortality. Thus, considering the complexity of virus transmission in indoor environment, the development of accurate viral bioburden assessments in health care contexts is unquestionably imperative in order to implement proper infection control strategies.

Depletion of DAND5 Hinders EMT in Mouse Embryonic Stem Cell Differentiation

Abstract: Dand5 encodes a protein that acts as an antagonist to Nodal/TGF-β and Wnt pathways. A mouse knockout (KO) model has shown that this molecule is associated with left-right asymmetry and cardiac development, with its depletion causing heterotaxia and cardiac hyperplasia.This study aimed to investigate the molecular mechanisms affected by the depletion of Dand5.DAND5-KO and wild-type embryoid bodies (EBs) were used to assess genetic expression with RNA sequencing. To complement the expression results that pointed towards differences in epithelial to mesenchymal transition (EMT), we evaluated migration and cell attachment. Lastly, in vivo valve development was investigated, as it was an established model of EMT.DAND5-KO EBs progress faster through differentiation. The differences in expression will lead to differences in the expression of genes involved with Notch and Wnt signalling pathways, as well as changes in the expression of genes encoding membrane proteins. Such changes were accompanied by lower migratory rates in DAND5-KO EBs, as well as higher concentrations of focal adhesions. Within valve development, Dand5 is expressed in the myocardium underlying future valve sites, and its depletion compromises correct valve structure.The DAND5 range of action goes beyond early development. Its absence leads to significantly different expression patterns in vitro and defects in EMT and migration. These results have an in vivo translation in mouse heart valve development. Knowledge regarding the influence of DAND5 in EMT and cell transformation allows further understanding of its role in development, or even in some disease contexts, such as congenital heart defects.

Fusion methods to find web communities

Abstract: We propose a new method of community identification based on the combination of different approaches (context and link analyses) using stable information user’s needs (profiles) and documents (as author’s profile). We discuss application, standards and identify gaps and needs in the process of web community’s identification.