Polytechnic Institute of Lisbon

About: Polytechnic Institute of Lisbon is a education organization based out in Lisbon, Portugal. It is known for research contribution in the topics: Monetary policy & New Keynesian economics. The organization has 261 authors who have published 368 publications receiving 3569 citations. The organization is also known as: Polytechnical Institute of Lisbon.

Localização de áreas para infiltração de águas residuais tratadas de pequenos aglomerados urbanos

Abstract: (1) ESTIG Instituto Politecnico de Braganca, Campus de Santa Apolonia, Apartado 1134, 5301-857 Braganca, Portugal, e-mail: flora@ipb.pt (2) Departamento de Engenharia Civil e Arquitectura, Universidade da Beira Interior, Edificio 2 das Engenharias, Calcada Fonte do Lameiro, 6201-001 Covilha, Portugal, e-mail: ajca@ubi.pt, cavaleiro@ubi.pt (3) Departamento de Engenharia Civil, Instituto Superior de Engenharia de Lisboa, Rua Conselheiro Emidio Navarro 1, 1959-007 Lisboa, Portugal, e-mail: hmarecos@dec.isel.ipl.pt

Formação em animação sociocultural: contributos de projectos de investigação e intervenção em animação socioeducativa sociocultural community development professionals' training: scientific research and social-educational intervention projects' contribution

Abstract: RESUMO: No presente artigo procura-se evidenciar a potencialidade do uso de saberes de natureza teorica, tecnica e pratica, resultantes da participacao em projectos de investigacao cientifica e intervencao para a formacao em animacao sociocultural ‐ mais concretamente na sua vertente socioeducativa. Com esta ilustracao pretende-se sublinhar a potencialidade que a mobilizacao desses saberes pode ter no âmbito da formacao dos futuros profissionais em animacao sociocultural, designadamente, no dominio cientifico, no dominio tecnico-metodologico, e ainda, no dominio da profissionalizacao deste grupo profissional. PALAVRAS-CHAVE: Animacao Sociocultural; Conhecimento Profissional; Formacao em animacao sociocultural.

Development of a biosensor for urea assay in wines by ion selective electrode based on amidase inhibition

Abstract: The aim of this work consists of development of a biosensor for urea based on amidase inhibition since urea is a powerful active site inhibitor of this enzyme which catalyses the hydrolysis of amides such as acetamide producing ammonia and the corresponding organic acid. Cell-free extract from Pseudomonas aeruginosa was the source of amidase (acylamide hydrolase EC 3.5.1.4) which was immobilized in polyethersulfone (PES) membrane in the presence glutaraldehyde and ion-selective electrode (ISE) for ammonium ions was used for biosensor development. ANOVA methodology was used for optimization of the biosensor response and hence 30 μL of cell-free extract, 2 μL glutaraldehyde (5%) and 10 μL gelatin 15% (p/v) exhibited the highest response. This biosensor exhibited a linear response in the range of 4.0 – 10.0 M of urea, a detection limit of 2.0 M for urea, a response time of 20 s, a sensitivity of 58.245 %.M -1 of urea concentration and a storage stability of over two months. It was successfully used for quantification of urea in real samples such as wine and recovery experiments were carried out which revealed an average substrate recovery of 93.1%. This biosensor is cheap since cell-free extract of P. aeruginosa can be used as source of amidase activity. The selectivity of this biosensor was investigated towards urea analogues such as hydroxyurea, methylurea and thiourea which revealed that they inhibited amidase activity about 90%, 10% and 0%, respectively compared with urea inhibition.

Single-stage stellarator optimization: Combining coils with fixed boundary equilibria

Abstract: Abstract We introduce a novel approach for the simultaneous optimization of plasma and coil engineering objectives in a fixed-boundary equilibrium that is computationally efficient and applicable to a broad range of vacuum and finite plasma pressure scenarios. Our approach treats plasma boundary and coil shapes as independently optimized variables, penalizing the mismatch between the two using a quadratic flux term in the objective function. Four use cases are presented to demonstrate the effectiveness of the approach, including simple and complex stellarator geometries. As it is shown here, this method outperforms previous 2-stage approaches, achieving smaller plasma objective function values when coils are taken into account.