Translational Research Institute

About: Translational Research Institute is a facility organization based out in Woolloongabba, Queensland, Australia. It is known for research contribution in the topics: Population & Cancer. The organization has 817 authors who have published 1163 publications receiving 25513 citations.

Nuclear Factor kappa B activation by Ag, Au nanoparticles, CdTe quantum dots or their binary mixtures in HepG2 cells.

Abstract: Introduction and objective Nuclear factor kappa B (NF-κB) signalling pathway plays a central role in the regulation of cellular response to stress. The aim of the study was to investigate the ability of silver nanoparticles (AgNPs), gold nanoparticles (AuNPs), CdTe quantum dots (CdTeQDs) or their binary mixtures to stimulate NF-κB binding in HepG2 cells. A dual luciferase reporter system was used to investigate NF-κB binding. Material and methods Cells were transiently transfected with a firefly luciferase reporter system and Renilla luciferase expression plasmid as a transfection efficiency control. Twenty- four hours after transfection, the cells were treated with nanoparticles (10 μg/cm3 AgNPs, 10 μg/cm3 AuNPs, 3 μg/cm3 CdTeQDs) or with 10 ng/cm3 TNFα as a positive control. Six hours later, the cells were lysed and the activities of the luminescence of firefly and Renilla luciferases were measured using the Dual-Luciferase Reporter Assay System. Results AuNPs and CdTeQDs alone significantly inhibited NF-κB binding activity. Co-treatment with AgNPs and CdTeQDs resulted in an additive effect, whereas the presence of AgNPs diminished the inhibitory effect of AuNPs. Interestingly, significant antagonism was observed between AuNPs and CdTeQDs, suggesting a similar mode of action. Conclusions Comparison of the NF-κB binding activity induced by the mixtures of NPs suggests that in some cases NF-κB binding activity might differ from that observed for the NPs alone.

A Preclinical Animal Model for the Study of Scaffold-Guided Breast Tissue Engineering.

Abstract: Scaffold-guided breast tissue engineering (SGBTE) has the potential to transform reconstructive breast surgery. Currently, there is a deficiency in clinically relevant animal models suitable for studying novel breast tissue engineering concepts. To date, only a small number of large animal studies have been conducted and characterization of these large animal models is poorly described in the literature. Addressing this gap in the literature, this publication comprehensively describes our original porcine model based on the current published literature and the experience gained from previous animal studies conducted by our research group. In a long-term experiment using our model, we investigated our SGBTE approach by implanting 60 additively manufactured bioresorbable scaffolds under the panniculus carnosus muscle along the flanks of 12 pigs over 12 months. Our model has the flexibility to compare multiple treatment modalities where we successfully investigated scaffolds filled with various treatments of immediate and delayed fat graft and augmentation with platelet rich plasma. No wound complications were observed using our animal model. We were able to grow clinically relevant volumes of soft tissue, which validates our model. Our preclinical large animal model is ideally suited to assess different scaffold or hydrogel-driven soft tissue regeneration strategies. Impact statement The ability to regenerate soft tissue through scaffold-guided tissue engineering concepts can transform breast reconstructive surgery. We describe an original preclinical large animal model to study controlled and reproducible scaffold-guided breast tissue engineering (SGBTE) concepts. This model features the flexibility to investigate multiple treatment conditions per animal, making it an efficient model. We have validated our model with a long-term experiment over 12 months, which exceeds other shorter published studies. Our SGBTE concept provides a more clinically relevant approach in terms of breast reconstruction. Future studies using this model will support the translation of SGBTE into clinical practice.

Implementation of PDOPPS in a middle-income country: Early lessons from Thailand.

Abstract: Background:Despite the implementation of a ‘Peritoneal Dialysis (PD) First’ policy in Thailand since 2008, nationwide PD practices and patients’ outcomes have rarely been reported.Methods:As part o...

Cerivastatin to treat pulmonary disorders

Abstract: Pharmaceutical formulations and methods of using thereof for the treatment and prevention of pulmonary arterial hypertension are provided. The formulations contain one or more agents to simultaneously reduce ADMA levels in a patient and reduce inflammatory processes in the pulmonary vasculature of a patient. The formulations contain a therapeutically effective amount of cerivastatin, a cerivastatin analog, or a pharmaceutically acceptable salt, prodrug, clathrate, or solvate thereof in a carrier suitable for pulmonary administration.

What Is the Optimal Dietary Composition for NAFLD

Abstract: Non-alcoholic fatty liver disease (NAFLD) is a complex condition with multi-organ considerations. Weight reduction effectively reduces steatosis in the short term; however, the effects of dietary composition in the absence of weight loss on hepatic and metabolic measures remain unclear. This review sought to update the evidence for whole of diet approaches and interventions in human studies of NAFLD. Assessing dietary composition in NAFLD is complex and requires consideration of food patterns across decades of life. Dietary composition aligned with mostly plant-based, high fibre, unprocessed foods, such as the Mediterranean Diet, can produce hepatic and metabolic benefits in NAFLD irrespective of weight loss. High fructose-sweetened beverages are associated with more severe steatosis, and short-term avoidance has demonstrated rapid improvements in liver health. Regular coffee drinking may have a protective effect against fibrosis in those with established NASH. Changing focus from single nutrient to dietary patterns may offer greater translation capability to research findings. Investment in whole food dietary education approaches to engage lifelong health behaviours targeted at culturally appropriate healthy food and lifestyle choices are needed.