Showing papers in "Asian pacific Journal of Tropical Biomedicine in 2015"

Essential oils used in aromatherapy: A systemic review

Abstract: Nowadays, use of alternative and complementary therapies with mainstream medicine has gained the momentum. Aromatherapy is one of the complementary therapies which use essential oils as the major therapeutic agents to treat several diseases. The essential or volatile oils are extracted from the flowers, barks, stem, leaves, roots, fruits and other parts of the plant by various methods. It came into existence after the scientists deciphered the antiseptic and skin permeability properties of essential oils. Inhalation, local application and baths are the major methods used in aromatherapy that utilize these oils to penetrate the human skin surface with marked aura. Once the oils are in the system, they remodulate themselves and work in a friendly manner at the site of malfunction or at the affected area. This type of therapy utilizes various permutation and combinations to get relief from numerous ailments like depression, indigestion, headache, insomnia, muscular pain, respiratory problems, skin ailments, swollen joints, urine associated complications etc. The essential oils are found to be more beneficial when other aspects of life and diet are given due consideration. This review explores the information available in the literature regarding therapeutic, medical, cosmetic, psychological, olfactory, massage aromatherapy, safety issues and different plants used in aromatherapy. All the available information was compiled from electronic databases such as Academic Journals, Ethnobotany, Google Scholar, PubMed, Science Direct, Web of Science, and library search.

Exposure to polycyclic aromatic hydrocarbons with special focus on cancer

Abstract: Polycyclic aromatic hydrocarbons (PAHs) are a group of compounds consisting of two or more fused aromatic rings. Most of them are formed during incomplete combustion of organic materials such as wood and fossil fuels, petroleum products, and coal. The composition of PAH mixtures varies with the source and is also affected by selective weathering effects in the environment. PAHs are ubiquitous pollutants frequently found in a variety of environments such as fresh water and marine sediments, the atmosphere, and ice. Due to their widespread distribution, the environmental pollution due to PAHs has aroused global concern. Many PAHs and their epoxides are highly toxic, mutagenic and/or carcinogenic to microorganisms as well as to higher forms of life including humans. The main aim of this review is to provide contemporary information on PAH sources, route of exposure, worldwide emission rate, and adverse effects on humans, especially with reference to cancer.

Nosocomial infections and their control strategies.

Abstract: Nosocomial infections are also known as hospital-acquired/associated infections. National Healthcare Safety Network along with Centers for Disease Control for surveillance has classified nosocomial infection sites into 13 types with 50 infection sites, which are specific on the basis of biological and clinical criteria. The agents that are usually involved in hospital-acquired infections include Streptococcus spp., Acinetobacter spp., enterococci, Pseudomonas aeruginosa , coagulase-negative staphylococci, Staphylococcus aureus , Bacillus cereus , Legionella and Enterobacteriaceae family members, namely, Proteus mirablis , Klebsiella pneumonia , Escherichia coli , Serratia marcescens . Nosocomial pathogens can be transmitted through person to person, environment or contaminated water and food, infected individuals, contaminated healthcare personnel's skin or contact via shared items and surfaces. Mainly, multi-drug-resistant nosocomial organisms include methicillin-resistant Staphylococcus aureus , vancomycin-resistant enterococci, Pseudomonas aeruginosa and Klebsiella pneumonia , whereas Clostridium difficile shows natural resistance. Excessive and improper use of broad-spectrum antibiotics, especially in healthcare settings, is elevating nosocomial infections, which not only becomes a big health care problem but also causes great economic and production loss in the community. Nosocomial infections can be controlled by measuring and comparing the infection rates within healthcare settings and sticking to the best healthcare practices. Centers for Disease Control and Prevention provides the methodology for surveillance of nosocomial infections along with investigation of major outbreaks. By means of this surveillance, hospitals can devise a strategy comprising of infection control practices.

Coriander (Coriandrum sativum L.) essential oil: Chemistry and biological activity

Abstract: Coriandrum sativum L. (C. sativum) is one of the most useful essential oil bearing spices as well as medicinal plants, belonging to the family Umbelliferae/Apiaceae. The leaves and seeds of the plant are widely used in folk medicine in addition to its use as a seasoning in food preparation. The C. sativum essential oil and extracts possess promising antibacterial, antifungal and anti-oxidative activities as various chemical components in different parts of the plant, which thus play a great role in maintaining the shelf-life of foods by preventing their spoilage. This edible plant is non-toxic to humans, and the C. sativum essential oil is thus used in different ways, viz., in foods (like flavouring and preservatives) and in pharmaceutical products (therapeutic action) as well as in perfumes (fragancias and lotions). The current updates on the usefulness of the plant C. sativum are due to scientific research published in different web-based journals.

Adansonia digitata L. (baobab): a review of traditional information and taxonomic description

Abstract: Adansonia digitata L. (Malvaceae) is commonly known as baobab tree native to Africa. Baobab is a multi-purpose tree which offers protection and provides food, clothing and medicine as well as raw material for many useful items. The fruit pulp, seeds, leaves, flowers, roots, and bark of baobab are edible and they have been studied by scientists for their useful properties. The fruit pulp have very high vitamin C, calcium, phosphorus, carbohydrates, fibers, potassium, proteins and lipids content, which can be used in seasoning as an appetizer and also make juices. Seeds contain appreciable quantities of phosphorus, magnesium, zinc, sodium, iron, manganese, whereas they have high levels of lysine, thiamine, calcium and iron. Baobab has numerous biological properties including antimicrobial, anti-malarial, diarrhoea, anaemia, asthma, antiviral, anti-oxidant and anti-inflammatory activities amongst others. Phytochemical investigation revealed the presence of flavonoids, phytosterols, amino acids, fatty acids, vitamins and minerals. The review summarizes the information on various aspects of traditional information, taxonomic description, medicinal properties and importantly nutritional value.

Medicinal plants with hepatoprotective activity in Iranian folk medicine

Abstract: There are a number of medicinal combinations in the Iranian traditional medicine which are commonly used as tonic for liver. In this review, we have introduced some medicinal plants that are used mainly for the treatment of liver disorders in Iranian folk medicine, with focus on their hepatoprotective effects particularly against CC14 agent. In this study, online databases including Web of Science, PubMed, Scopus, and Science Direct were searched for papers published from January 1970 to December 2013. Search terms consisted of medicinal plants, traditional medicine, folk medicine, hepatoprotective, Iran, liver, therapeutic uses, compounds, antioxidant, CC14, anti-inflammatory, and antihepatotoxic, hepatitis, alone or in combination. Allium hirtifolium Boiss., Apium graveolens L., Cynara scolymus, Berberis vulgaris L., Calendula officinalis, Nigella sativa L., Taraxacum officinale, Tragopogon porrifolius, Prangos ferulacea L., Allium sativum, Marrubium vulgare, Ammi majus L., Citrullus lanatus Thunb, Agrimonia eupatoria L. and Prunus armeniaca L. are some of the medicinal plants that have been used for the treatment of liver disorders in Iranian folk medicine. Out of several leads obtained from plants containing potential hepatoprotective agents, silymarin, β-sitosterol, betalain, neoandrographolide, phyllanthin, andrographolide, curcumin, picroside, hypophyllanthin, kutkoside, and glycyrrhizin have been demonstrated to have potent hepatoprotective properties. Despite encouraging data on possibility of new discoveries in the near future, the evidence on treating viral hepatitis or other chronic liver diseases by herbal medications is not adequate.

Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria

Abstract: Objective To investigate the antibacterial activities of crude ethanol extracts of 12 Philippine medicinal plants. Methods Crude ethanol extracts from 12 Philippine medicinal plants were evaluated for their antibacterial activity against methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, extended spectrum β-lactamase-producing, carbapenem-resistant Enterobacteriaceae and metallo-β-lactamase-producing Pseudomonas aeruginosa and Acinetobacter baumannii. Results The leaf extracts of Psidium guajava, Phyllanthus niruri, Ehretia microphylla and Piper betle (P. betle) showed antibacterial activity against the Gram-positive methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus. P. betle showed the highest antibacterial activity for these bacteria in the disk diffusion (16–33 mm inhibition diameter), minimum inhibitory concentration (19–156 μg/mL) and minimum bactericidal concentration (312 μg/mL) assays. P. betle leaf extracts only showed remarkable antibacterial activity for all the Gram-negative multidrug-resistant bacteria (extended spectrum β-lactamase-producing, carbapenem-resistant Enterobacteriaceae and metallo-β-lactamase-producing) in the disk diffusion (17–21 mm inhibition diameter), minimum inhibitory concentration (312–625 μg/mL) and minimum bactericidal concentration (312–625 μg/mL) assays. Conclusions P. betle had the greatest potential value against both Gram-negative and Gram-positive multidrug-resistant bacteria. Favorable antagonistic activities were also exhibited by the ethanol extracts of Psidium guajava, Phyllanthus niruri and Ehretia microphylla.

The past, current and future trends in DNA vaccine immunisations

Abstract: This review focuses on DNA vaccines, denoting the last two decades since the early substantiation of preclinical protection was published in Science in 1993 by Ulmer et al. In spite of being safely administered and easily engineered and manufactured DNA vaccine, it holds the future prospects of immunization by inducing potent cellular immune responses against infectious and non-infectious diseases. It is well documented that injection of DNA plasmid encoding a desired gene of interest can result in the subsequent expression of its products and lead to the induction of an immune response within a host. This is pertinent to prophylactic and therapeutic vaccination approach when the peculiar gene produces a protective epitope from a pathogen. The recent studies demonstrated by a number of research centers showed that these immune responses evoke protective immunity against several infectious diseases and cancers, which provides adequate support for the use of this approach. We attempt in this review to provide an informative and unbiased overview of the general principles and concept of DNA vaccines technology with a summary of a novel approach to the DNA vaccine, present investigations that describe the mechanism(s) of protective immunity provoked by DNA immunization and to highlight the advantages and disadvantages of DNA immunisation.

Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles mediated by Eucalyptus camaldulensis leaf extract

Abstract: Objective To investigate the environmental-friendly extracellular biosynthetic technique for the production of the silver nanoparticles (AgNPs) by using leaf extract of Eucalyptus camaldulensis ( E. camaldulensis ). Methods The NP were characterized by colour changes and the UV-visible spectroscopy. The cytotoxic effects of prepared AgNPs was detected against four types of pathogenic bacteria, including two Gram-negative bacteria ( Pseudomonas aeruginosa and Escherichia coli ) and two Gram-positive bacteria ( Staphylococcus aureus and Bacillus subtilis ) by using agar well diffusion method. Results A peak absorption value between 400-450 nm for the extract and the colour change to dark brown were corresponding to the plasmon absorbance of AgNPs. On the other hand, aqueous extract of E. camaldulensis leaves could be effective against tested microorganisms which showed inhibition zones of 9.0-14.0 mm. Furthermore, biologically synthesized AgNPs had higher ability to suppress the growth of the tested microorganisms (12.0-19.0 mm). Conclusions Our findings indicated that extracellular synthesis of AgNPs mediated by E. camaldulensis leaf extract had an efficient bactericidal activity against the bacterial species tested. The exact mechanism of the extracellular biosynthesis of metal NP was not well understood. Further studies are needed to highlight the biosynthesis process of AgNPs and also to characterize the toxicity effect of these particles.

Antidiabetic claims of Tinospora cordifolia (Willd.) Miers: critical appraisal and role in therapy

Abstract: Currently, available conventional options for diabetes mellitus have certain limitations of their own, and options from medicinal plants with antihyperglycemic activities are being searched to meet the need. Antidiabetic properties of Tinospora cordifolia are highly appreciated in Ayurveda and even in recent modern researches. Several studies on its extracts (viz. immunemodulatory, anti-hyperglycemic, antioxidant, adaptogenic, hepatoprotective, hormone regulator etc.) and isolated phytoconstituents (like tinosporin, berberine, jatrorrhizine etc.) have reported that it is a preventive and curative antidiabetic herb, which are substantiated by clinical trials. Scattered information pertaining to antidiabetic potential of Tinospora is reported. Present review encompasses (i) in-depth information of reported antidiabetic activities of the plant in light of available experimental and clinical studies, and (ii) understanding on the possible mechanism of its action in combating the complex pathology of diabetes.

Evaluating patients' perception of service quality at hospitals in nine Chinese cities by use of the ServQual scale

Abstract: Objective To investigate patients' perception of service quality at hospitals in nine Chinese cities and propose some measures for improvement. Methods The ServQual scale method was used in a survey involving patients at out-patient and in-patient facilities in Shanghai, Chongqing, Chengdu, Nanning, Guilin and Laibin of Guangxi, Honghezhou of Yunnan, Wulumuqi of Xinjiang and Zhongshan of Guangdong. The data collected were entered and analyzed using SPSS 20.0. Statistical analyses included descriptive statistics, factor analyses, reliability analyses, product-moment correlations, independent-sample t -tests, One-way ANOVA and regression analyses. Results The Kaiser-Meyer-Olkin value for the factor analysis of the scale was 0.979. The Cronbach's α for the reliability analysis was 0.978. All the Pearson correlation coefficients were positive and statistically significant. Visitors to out-patient facilities reported more positive perception than visitors to in-patient facilities on tangibles ( t = 4.168, P t = 1.979, P F = 3.311, P = 0.010), assurances ( F = 2.751, P F = 4.009, P = 0.003). For the five dimensions of the scale, patients in Laibin, Guangxi reported the most positive perceived service quality, followed by patients in Shanghai. On the other hand, patients in Chongqing and Nanning and Guilin of Guangxi reported relatively poor perceptions of service quality. Standardized regression coefficients showed statistically significant ( P Conclusions Chinese patients perceived service quality as satisfactory. Hospitals in various regions of China should enhance their awareness and ability to serve their patients.

Ethnobotanical inventory and folk uses of indigenous plants from Pir Nasoora National Park, Azad Jammu and Kashmir

Abstract: Objective To document the medicinal and other folk uses of native plants of the area with a view to preserve the ethnobotanical knowledge associated with this area. Methods The fieldwork was conducted during a period of one year. Data were collected through a semi-structured questionnaire and interviews with indigenous tribal people and traditional health practitioners residing in the study area. Results The present study documented ethnobotanical uses of 104 plant species belonged to 93 genera and 51 families. Results revealed that most of the documented species were used medicinally (78 spp., 44.07%). Leaves were found to be the most frequently used part (69 spp., 42.86%) for the preparation of indigenous recipes and for fodder. Conclusions The current research contributes significantly to the ethnobotanical knowledge, and depicts a strong human-plant interaction. There is an urgent need to further document indigenous uses of plants for future domestication.

Amelioration of paracetamol hepatotoxicity and oxidative stress on mice liver with silymarin and Nigella sativa extract supplements

Abstract: Objective To evaluate the ameliorator property of silymarin or/and Nigella sativa (N. sativa) extract against N-acetyl-p-aminophenol (APAP)-induced injury in male mice at the biochemical, histological and ultrastructural levels. Methods The mice were divided into seven groups (10/group). The first group was served as control. While, the second group was treated with dose of APAP. The third and fourth groups were treated with silymarin alone and N. sativa extract alone respectively. The fifth and sixth groups were treated with combination of APAP with silymarin and APAP with N. sativa extract respectively. The seventh group was treated with combination of both ameliorative compounds (silymarin and N. sativa extract) with APAP and all animals were treated for a period of 30 days. Results Exposure to APAP at the treated dose to mice led to an alteration of liver functions, increased the alanine transaminase, aspartate aminotransferase and lactate dehydrogenase levels, decreased total protein level as well as increasing the superoxide dismutase and malondialdehyde while decreased catalase, glutathione peroxidase and glutathione reduced activities. The levels of APAP on the biochemical parameters of mice were dose-dependent. Administration of silymarin or/and N. sativa extract to APAP-treated mice attenuates the toxicity of this compound, objectified by biochemical, histological and ultrastructural improvement of liver. But the alleviation was more pronounced with the both antioxidants. Conclusions The synergistic effect of silymarin and N. sativa extract is the most powerful in reducing the toxicity induced by APAP and improving the liver functions and antioxidant capacities of mice.

Anti-inflammatory, anticoagulant and antioxidant effects of aqueous extracts from Moroccan thyme varieties

Abstract: Objective To evaluate the anti-inflammatory, anticoagulant and antioxidant effects of aqueous extracts of thyme varieties from Moroccan. Methods The aqueous extracts of tree medicinal plants [ Thymus atlanticus ( T. atlanticus ), Thymus satureioides and Thymus zygis ( T. zygis )] were screened for their antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical-scavenging, ferric reducing antioxidant power assay, radical scavenging activity method, the inhibition of 2,2'-azobis (2-amidinopropane) dihydrochloride that induces oxidative erythrocyte hemolysis and thiobarbituric acid reactive substances assay. The anti-inflammatory activity of aqueous extracts was evaluated in vivo using croton oil-induced ear edema and carrageenan-induced paw edema in mice and rats, respectively. This extracts were evaluated in vitro for their anticoagulant activity at the different concentrations by partial thromboplastin time and prothrombin time activated. Results All thyme varieties were found to possess considerable antioxidant activity and potent anti-inflammatory activity in the croton oil-induced edema. Administration of aqueous extracts of two varieties (50 mg/kg) ( T. zygis and T. atlanticus ) reduced significantly the carrageenan-induced paw edema similar to non-steroidal anti-inflammatory drug (indomethacin, 10 mg/kg). In partial thromboplastin time and prothrombin time tests, T. atlanticus and T. zygis extracts showed the strongest anticoagulant activity. In contrast, Thymus satureioides did not show the anticoagulant activity in these tests. Conclusions All aqueous extracts possess considerable antioxidant activity and are rich in total polyphenol and flavonoid but they act differently in the process of inflammatory and coagulation studied. This study shows great variability of biological activities in thyme varieties.

Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells

Abstract: Objective To investigate the in vitro anticancer effect of Aloe vera ( A. vera ) and Calligonum comosum ( C. comosum ) extracts against hepatocellular carcinoma (HepG2) cells. Methods HepG2 cells were tested against different doses of A. vera and C. comosum . Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy. Results The IC 50 values of A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) µg/mL respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions. Conclusions The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.

Phytochemical analysis, hypotensive effect and antioxidant properties of Myrtus communis L. growing in Algeria

Abstract: Objective To analyze Myrtus communis chemically and evaluate the hypotensive effects and antioxidant properties of methanol, chloroform, ethyl acetate and aqueous extracts from the leaves of this plant Methods Total phenolic and flavonoid contents as well as the antioxidant potential of methanol, chloroform, ethyl acetate and aqueous extracts have been investigated by using different in vitro methods The hypotensive effects of methanol and ethyl acetate extracts were evaluated in anaesthetized rats by using the method of invasive blood pressure recording Moreover, ethyl acetate extract was subjected to analysis by different chromatographic methods in order to identify new compounds Results Chemical analysis of ethyl acetate extract revealed the presence of myrecitin-3-O-α-rhamnoside Ethyl acetate extract was found to have the highest total phenolic and total flavonoid contents with the values of 43537 mg gallic acid equivalents/g dried weight and 13075 mg quercetin equivalent/g dried weight, respectively Ethyl acetate extract also exhibited the highest activity in scavenging 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid), hydroxyl radical and reducing power; whereas, methanol extract exhibited higher chelating activity than ethyl acetate extract did Chloroform was found to be strong inhibitor of lipid peroxidation in β-carotene bleaching assay (9119%), ferric thiocyanate method (8755%), and thiobarbituric acid method (8259%) as compared to butylated hydroxytoluene Intravenous administration of methanol and ethyl acetate extract (004 to 12 mg/kg body weight) decreased the maximum mean arterial blood pressure with values of 206% and 3249% at 12 mg/kg body weight, respectively in anesthetized rats Conclusions This study provides a scientific basis for the use of Myrtus communis in traditional medicine as hypertensive agent as well as additional resources for natural antioxidants

Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

Abstract: Objective To compare the in vitro antioxidant capacity of a diatom, Chaetoceros calcitrans (C. calcitrans) extracted using six types of solvents. Methods Each extract was evaluated in terms of extraction yield, total carotenoid, fucoxanthin content, total phenolic and antioxidant capacities (DPPH and ABTS + scavenging activity and iron chelating activity). Results The methanol extract exhibited the highest yield [(22.71 ± 0.96) g/100 g dry weight (DW)], total carotenoid [(4.46 ± 0.36) mg/g DW], total phenolic [(2.49 ± 0.08) mg gallic acid equivalents/g DW] and second highest fucoxanthin content [(2.08 ± 0.03) mg fucoxanthin/g DW] as compared to other solvent extracts. Methanolic extract also exhibited significantly higher (P , ABTS +) and iron chelating activities. Conclusions Methanol was the recommended solvent for the production of antioxidant rich extract from C. calcitrans. Both carotenoids and phenolic acids were found to be positively correlated to the antioxidant capacities of C. calcitrans. Lead bioactives confirmed by subsequent high performance liquid chromatography studies were fucoxanthin, gallic acid and protocatechuic acid.

Screening for anti-pancreatic lipase properties of 28 traditional Thai medicinal herbs

Abstract: Objective To screen the effect of 28 medicinal plants on inhibition of pancreatic lipase and evaluate the phytochemical contents of extracts. Methods The ethanolic extracts of 28 traditional Thai herbal medicines were assayed for their in vitro activities against porcine pancreatic lipase using p -nitrophenyl butyrate as a substrate. Quantitative estimation of flavonoids, phenolics, and alkaloids was done. Results Extracts from four herbs, Memecylon edule Roxb., Garcinia vilersiana Pierre, Cryptolepis elegans Wall. and Phyllanthus chamaepeuce Ridl., at a concentration of 100 μg/mL, strongly inhibited porcine pancreatic lipase by 90.97%, 92.04%, 94.64% and 95.38%, respectively. There was a significant positive correlation between phenolic content and inhibition activity. Inhibition activity was significantly correlated with flavonoid and with alkaloid contents. Conclusions From this result, it could be concluded that herbs represent a rich of anti-pancreatic lipase compounds, in particular, Cryptolepis elegans Wall. and Phyllanthus chamaepeuce Ridl. It is suggested that the phytochemical compounds from these plants may be applied for the prevention and treatment of obesity or hyperlipidemia.

Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco.

Abstract: Objective To investigate antioxidant activity, total phenolic and flavonoid content of ethanol (E) and ethyl acetate (A) extracts of Inula viscosa aerial parts selected from three regions of Morocco (Imouzzer, Sefrou and Taounate). Methods Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl, total antioxidant capacity and iron-reducing capacity. Total phenolic content was measured by Folin-Ciocalteu reagent. Results All the extracts showed significant antioxidant activities and contained important levels of phenols. The ethanol extract (0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay, which was probably due to its high content of polyphenols (274.39±6.94) mg gallic acid equivalent/g dry extract. Total flavonoid content was found equal for all extracts. Conclusions Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants. The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.

Hypoglycemic and antioxidant activities of Caesalpinia ferrea Martius leaf extract in streptozotocin-induced diabetic rats

Abstract: Objective To evaluate the antidiabetic and antioxidant effects of aqueous ethanolic extract of Caesalpinia ferrea ( C. ferrea ) leaf in normal and streptozotocin (STZ) induced diabetic rats. Methods Male Sprague-Dawley rats divided into 6 groups of 6 rats each were assigned into diabetic and non-diabetic groups. Diabetes was induced in rats by single intraperitoneal administration of STZ (65 mg/kg body weight). C. ferrea extract at the doses of 250 and 500 mg/kg body weight was orally administered to both diabetic and non-diabetic animals for a period of 30 days. After completion of experimental duration serum, liver and pancreas were used for evaluating biochemical and histopathological changes. Results Oral administration of C. ferrea leaf extract significantly reduced elevated serum glucose, α-amylase, liver function levels and significantly increased serum insulin, total protein and body weight as well as improved lipid profile due to diabetes. Furthermore, the treatment resulted in a marked increase in glutathione peroxidase, superoxide dismutase, catalase and reduced glutathione, and diminished levels of lipid peroxidation in liver and pancreas of diabetic rats. Histopathological studies demonstrated the reduction in the pancreas and liver damage and confirmed the biochemical findings. Conclusions From the present study, it can be concluded that the C. ferrea leaf extract effectively improved hyperglycaemia while inhibiting the progression of oxidative stress in STZ-induced diabetic rats. Hence, it can be used in the management of diabetes mellitus.

Lantana camara berry for the synthesis of silver nanoparticles

Abstract: Objective To synthesize the silver nanoparticles (AgNPs) by reduction of silver ions into nano silver, using ripened berry extract of Lantana camara and evaluate its antioxidant activity against 1, 1-diphenyl-2- picrylhydrazyl. Methods The prepared AgNPs were characterized by visual, UV-visible spectrophotometer, dynamic light scattering and transmission electron microscopy with selected area electron diffraction. Results Transmission electron microscopy and dynamic light scattering analysis confirmed the AgNPs are spherical and 75.2 nm average sized. Selected area electron diffraction analysis supports that the obtained nanoparticles were in crystalline form. In addition, the antioxidant efficacy of prepared AgNPs was found to be higher than berry extract against 1, 1-diphenyl-2- picrylhydrazyl. Conclusions From the results obtained it is suggested that surface modified AgNPs at lower concentration, showed higher antioxidant activity than berry extract against 1, 1-diphenyl-2- picrylhydrazyl and could be used effectively in future ethno pharmacological concerns.

Anti-diabetic potential of Urena lobata leaf extract through inhibition of dipeptidyl peptidase IV activity

Abstract: Objective To evaluate the anti-diabetic potential of leaf extract from Urena lobata ( U. lobata ) through dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Methods U. lobata leaf was extracted in hot water and ethanol. The activity of DPP-IV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPP-IV and vildagliptin, as standard reference. A product of the reactions between gly-pro-p-nitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC 50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC 50 values of 1 654.64 and 6 489.88 μg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC 50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol.

The biomedical significance of the phytochemical, proximate and mineral compositions of the leaf, stem bark and root of Jatropha curcas

Abstract: Objective To analyse the phytochemical contents of leaf, stem bark and root of Jatropha curcas (J. curcas) in four solvent extracts and their proximate and mineral compositions. Methods Standard analytical procedures were used for the determination of phytochemicals, proximate and mineral compositions of the leaf, stem bark and root extracts of J. curcas. Results Results of the analysis showed the presence of polyphenols, flavonoids, alkaloids, cardiac glycosides, coumarins, saponins, terpenoids, steroids, triterpenoid saponins, carotenoids, phlobatannins and tannins in the leaf, stem bark and root of all the solvent extracts. Flavonoids were present in the highest amount in the ethyl acetate extracts of the leaf (7.35% ± 0.02%), stem bark (4.12% ± 0.01%) and root (3.35% ± 0.02%) followed by polyphenols in the methanol extracts of leaf (4.62% ± 0.02%), stem bark (2.77% ± 0.05%) and root (2.49% ± 0.02%). Poly-acetylated compounds were absent in all the solvent extracts of the leaf, stem bark and root. However, some anti-nutritional agents such as oxalates, phytates and cyanates were present in all the solvent extracts of the leaf, stem bark and root except the ethyl acetate. Phytates were high in the aqueous solvent of the leaf (6.12% ± 0.00%) but low in the stem bark (1.00% ± 0.05%) and root (0.89% ± 0.03%). Proximate composition showed appreciable amounts of total carbohydrate (36.33% ± 0.72%), crude protein (26.00% ± 0.47%) and reducing sugars (5.87% ± 0.14%) in the leaf, while crude fat was more in the stem bark (16.70% ± 0.30%). There was corresponding substantial energy in the leaf [(1514.77 ± 20.87) kJ/100 g] and stem bark [(907.00 ± 8.52) kJ/100 g]. Moisture and ash contents of the leaf, stem bark and root were within acceptable limits for the use in drugs formulation. The mineral composition showed substantial amounts of important elements such as Fe, Ca, Na, Mg and Zn. Others were P, K and Se. Conclusions The outcome of this study suggests that the leaf, stem bark and root of J. curcas have very good medicinal potentials, meet the standard requirements for drug formulation and serve as good sources of energy and nutrients except for the presence of some anti-nutritional elements predominant in the leaf.

Identification of medicinal plants effective in infectious diseases in Urmia, northwest of Iran

Abstract: Objective To identify the medicinal plants effective in infectious diseases. Methods Initially, we obtained a list of herbalists and traditional healers from Food and Drug Deputy. Direct observations and interviews as well as collection of herbarium specimens of indigenous medicinal plants effective in infectious diseases of urinary tract, reproductive, digestive, respiratory and skin systems were performed. This study was conducted through questionnaires and interviews; the questionnaires were distributed among traditional healers and simultaneous interviews were also run. The plants were herbariumized, herbarium specimens were authenticated, and their species were determined by using reliable flora and other sources. Finally, the data were input into Excel 2010 and analyses were performed. Results Out of the studied plants, 35 native medicinal plants belonging to 17 families were effective in the treatment of various diseases and infections. In this study, the Lamiaceae family had the highest frequency of plants for the treatment of infections. Traditional healers of Urmia in 24% of cases used the leaves of medicinal herb to treat patients. In 68% of cases, they prescribed medicinal herbs in the boiled forms. Most medicinal herbs showed therapeutic effect on the digestive system. Conclusions Traditional medicinal sources, valuable knowledge of traditional healers in Urmia, the scientific investigation of the effects of the herbs offered in this study and their effects in traditional medicine may provide a good source for new drugs in modern medicine.

Identification and preliminary characterization of non-polyene antibiotics secreted by new strain of actinomycete isolated from sebkha of Kenadsa, Algeria

Abstract: Objective To study the antimicrobial activity of actinomycetes isolated from sabkha of Kenadsa and identification of the isolate interesting. Methods Eighteen strains were isolated, using four culture media from sebkha of Kenadsa (Bechar, Southwestern Algeria). Screening of antimicrobial activity consisted of two steps: in primary screening, antibacterial activity was determined by using the agar plug method against test strains; in secondary screening, better isolate which showed a good activity in the first screening was selected to extract antimicrobial substances. The antimicrobial activities of extracts were evaluated by using Kirby–Bauer disc diffusion method. Partial characterization of antimicrobial products was performed on the basis of chemical revelations, UV–vis spectrometry and infrared spectroscopy. The identification of isolate interesting was performed through morphological, chemical, biochemical and physiological characteristics. Results All isolates showed antimicrobial activity against at least one microorganism test. One isolate, LAM143cG3, was selected for its broad spectrum and high antimicrobial activity. The isolate LAM143cG3 was identified as Spirillospora sp. The comparison between the species of this genus (Spirillospora rubra and Spirillospora albida) and our isolate indicated the existence of several physiological and biochemical differences which led us to suppose that this was a new member of this genus. Primary characterization of antimicrobial substances produced by the isolate LAM143cG3 indicated the presence of amines and phenols. The UV–vis spectrum suggested a non-polyenic nature of substances secreted by our isolate, while infrared confirmed the presence of amine groups. Conclusions The result of the present study revealed that sebkha of Kenadsa was rich in rare actinomycetes, that secreted interesting antimicrobial substance.

Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces

Abstract: Objective To investigate the true incidence of Bacillus cereus (B. cereus) in food and children diarrhea cases. Methods A total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the presence of B. cereus by selective plating on mannitol-egg-yolk-polymyxin agar. Confirmation of B. cereus was carried out by biochemical tests and PCR. Identification of non-B. cereus isolates was carried out by 16S rDNA sequencing. Antimicrobial susceptibility was done by disk diffusion method. Results Overall 35 samples (31.8%, n = 110) yielded Bacillus-like growth. Of which 19 samples (54.28%) were positive for B. cereus. All isolates were positive for enterotoxin production. No psychrotolerant B. cereus strains were detected in all samples. All B. cereus isolates were resistant to penicillin G, but susceptible to vancomycin, erythromycin and clindamycin. Conclusions The results of this study confirm the importance of including B. cereus in disease control and prevention programs, as well as in routine clinical and food quality control laboratories in both Saudi Arabia and Egypt.

Isolation, serotype diversity and antibiogram of Salmonella enterica isolated from different species of poultry in India

Abstract: Objective To study the occurrence and serotype diversity of Salmonella isolates in different species of poultry (chicken, emu and duck) and determine their resistance pattern against various antibiotics of different classes. Methods About 507 samples comprising 202 caecal contents and 305 fecal samples from chicken, emu and duck were processed for isolation of Salmonella enterica. Salmonellae were isolated and detected by standard protocol of ISO 6579 Amendment 1: Annex D. Genetic confirmation was also made by using 16S rRNA genus specific PCR. Serotype specific PCR was also done to detect the most common serovars viz. Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Gallinarum. All obtained isolates were subjected to a set of 25 antibiotics to study their antibiogram by using Baeur–Kirby disk diffusion method. Results Out of 507 samples processed, 32 isolates of Salmonella enterica (18 from caecal contents and 14 from faecal samples) were obtained, of which 24 belonged to 6 different serovars, 6 were untypeable and 2 were rough strains. Salmonella Enteritidis was the most predominant serotype (9), followed by Salmonella Typhimurium (5), Salmonella Virchow (4), Salmonella Gallinarum (3), Salmonella Reading (2) and Salmonella Altona (1). Antibiotic resistance pattern was maximum (100%) to oxacillin, penicillin and clindamycin, followed by ampicillin (68.75%), tetracycline (65.62%), nalidixic acid (56.25%) and colistin (46.87%). High sensitivity of isolates was recorded for chloramphenicol (96.87%) followed by meropenem (84.37%). Conclusions Occurrence of high proportion of serovars in our study which can cause serious gastroenteritis in humans is a matter of concern. Salmonella Altona has been detected for the first time in India from poultry. This serotype is known to cause serious outbreaks of gastroenteritis in humans. Multidrug resistant isolates were recovered at high percentage which can be attributed to non-judicious use of antibiotics both in prophylaxis and treatment regimen. This observation draws serious attention as poultry serves as an important source of transmission of these multidrug resistant Salmonella serovars to humans.

Ebola virus disease: past, present and future

Abstract: Ebola virus disease is one of the most deadly ailments known to mankind due to its high mortality rate (up to 90%) accompanying with the disease. Ebola haemorrhagic fever (EHF) is an infectious disease of animal that can be transmitted to both human and non-human primates. The first epidemic of EHF occurred in 1976 in the Democratic Republic of the Congo. The incubation period of ebola is less than 21 days. Ebola virus infections are depicted by immune suppression and a systemic inflammatory response that leads to damage of the vascular, coagulation and immune systems, causing multi-organ failure and shock. Five genetically distinct members of the Filoviridae family responsible for EHF are as follows: Zaire ebolavirus, Sudan ebolavirus, Cote d’Ivoire ebolavirus, Bundibugyo ebolavirus and Reston ebolavirus. The ongoing 2014 West Africa ebola epidemic has been considered as the most serious panic in the medical field with respect to both the number of human cases and death toll. The natural host for ebola virus is unknown, thus it is not possible to carry out programs to regulate or abolish virus from transmission to people. The ebola virus infection provides little chance to develop acquired immunity causing rapid progression of the disease. It is pertinent to mention that at present, there is no antiviral therapy or vaccine that is helpful against ebola virus infection in humans. The impediment of EHF necessitates much better understanding of the epidemiology of the disease, particularly the role of wildlife, as well as bats, in the spread of ebola virus to humans.

Ameliorative effect of zinc oxide and silver nanoparticles on antioxidant system in the brain of diabetic rats

Abstract: Objective To test the ability of both zinc oxide nanoparticles (ZnONPs) and silver nanoparticles (SNPs) to ameliorate the oxidative stress resulted from diabetes in diabetic rats. Methods Fifty male albino rats were used; ten of them were served as control group and forty, as the experiment group, were injected with streptozotocin at the single intraperitoneal dose of 100 mg/kg. Then, the experiment group was subdivided into, diabetic, diabetic + ZnONPs, diabetic + SNPs and diabetic + insulin groups. The activities and mRNA expression levels of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were determined in brain tissues. Malondialdehyde, total antioxidant capacity, zinc and silver concentrations were estimated in the brain tissues of all rats. Results A significant increase in the activities and mRNA expression levels of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase was shown. Malondialdehyde levels were significantly decreased while there was a significant increase in the zinc, silver concentrations and total antioxidant capacity in brain of ZnONPs and SNPs treated rats, compared with diabetic or diabetic + insulin group and their control group. Conclusions ZnONPs and SNPs can be used to ameliorate the oxidative stress in brain resulted from diabetes mellitus.

A review on metastatic breast cancer in Iran

Abstract: Metastatic breast cancer is a disease of early breast cancer that usually occurs several years after the early breast cancer. Breast cancer is the most common cancer among Iranian women. According to the new statistics in Iran 6160 breast cancers are diagnosed in the country each year and 1063 cases lead to death. In this paper, epidemiology, diagnosis and treatment have been investigated. In this study, case–control clinical trials and open studies with adequate data were collected. Due to the higher risk of age group 40–49 years and the advent of advanced breast cancer in Iranian women, the early diagnosis and determination of the exact size of the tumor before surgery is important in choosing a therapy plan. The decision on the therapy of invasive breast cancer depends on several factors such as cancer stage, tumor size and type, pathological and cytological status of the tumor, the patient's opinion, the presence or absence of estrogen and progesterone receptors in the cytoplasm of tumor cells and so on.