Thermal denaturation of globular proteins. Fourier transform-infrared studies of the amide III spectral region
G. Anderle,R. Mendelsohn +1 more
Reads0
Chats0
TLDR
The large number of observed bands suggests that slight variations in helical geometry, symmetry, or interactions result in changed amide III frequencies, so that simple correlations between narrow frequency ranges and secondary structures may not be applicable for this mode.About:
This article is published in Biophysical Journal.The article was published on 1987-07-01 and is currently open access. It has received 133 citations till now. The article focuses on the topics: Bovine serum albumin & Chymotrypsinogen.read more
Citations
More filters
Journal ArticleDOI
The Use and Misuse of FTIR Spectroscopy in the Determination of Protein Structure
TL;DR: This review critically assess the application of FTIR spectroscopy to the determination of protein structure by outlining the principles underlying protein secondary structure determination by FTIRSpectroscopy, and highlighting the situations in which FTIR Spectroscopy should be considered the technique of choice.
Book ChapterDOI
Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. I. Assignments and model compounds.
TL;DR: In this article, the authors reviewed the basic knowledge accumulated over the last twenty years on the different vibrations of polypeptides and pointed out that interpretation of the results still needs caution.
Book ChapterDOI
Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. III. Secondary structures.
Journal ArticleDOI
Identification of β-turn and random coil amide III infrared bands for secondary structure estimation of proteins
Shuowei Cai,Bal Ram Singh +1 more
TL;DR: This paper describes, for the first time, identification of amide III bands corresponding to beta-turns and random coil by selectively enhancing random coils by treatment with a denaturing reagent, and secondary structure estimation of several proteins by using the band assignments.
References
More filters
Journal ArticleDOI
Examination of the secondary structure of proteins by deconvolved FTIR spectra.
D M Byler,H. Susi +1 more
TL;DR: Fourier transform ir (FTIR) spectra of 21 globular proteins have been obtained, revealing that the amide I band of each protein except casein consists of six to nine components, although all proteins do not exhibit components at every characteristic frequency.
Journal ArticleDOI
Automatic identification of secondary structure in globular proteins
Michael Levitt,Jonathan Greer +1 more
TL;DR: This work provides the first objective and consistent compilation of α-helix, β-sheet and reverse-turn secondary structure in almost all globular proteins of known tertiary structure.
Journal ArticleDOI
Laser-excited Raman spectroscopy of biomolecules: I. Native lysozyme and its constituent amino acids☆
R.C. Lord,Nai-teng Yu +1 more
TL;DR: Laser-excited Raman spectra of a simple native protein, lysozyme, in aqueous solution are reported and partially interpreted with the help of the specta of its constituent amino acids and appear to be potentially useful in assessment of conformational changes caused by denaturation.
Book ChapterDOI
Infrared membrane spectroscopy.
U. P. Fringeli,Hs.H. Günthard +1 more
TL;DR: There have been many treatments of the theoretical basis of molecular vibrational spectroscopy, and more recently a comprehensive discussion has been presented by Bellamy (1975), which accentuate the correlation of vibratory spectra with molecular structure from an essentially empirical point of view.
Related Papers (5)
Examination of the secondary structure of proteins by deconvolved FTIR spectra.
D M Byler,H. Susi +1 more