André Guillouzo

TL;DR: This review brings you up-to-date with the hepatocyte research on in vitro–in vivo correlations of metabolism and clearance, the function and regulation of hepatic transporters and models used to elucidate their role in drug clearance, mechanisms and examples of idiosyncratic and intrinsic hepatotoxicity.

TL;DR: HepaRG cells constitute the first human hepatoma cell line expressing high levels of the major P450s involved in xenobiotic metabolism and represent a reliable surrogate to human hepatocytes for drug metabolism and toxicity studies.

TL;DR: Recent results obtained with a new human hepatoma cell line, named HepaRG, derived from a human hepatocellular carcinoma, which could represent a surrogate to primary human hepatocytes for xenobiotic metabolism and toxicity studies and even more, a unique model system for analysing genotoxic compounds.

TL;DR: The results demonstrate for the first time long-term stabilization and reversibility of a specific function (albumin secretion) at high levels by adult hepatocytes cultured in serum-free medium and suggest that both the presence of other liver cell type(s) and the production of an extracellular matrix are needed for the maintenance of specific functions in cultured hepatocytes.

TL;DR: These results provide the first demonstration that various cytokines act directly on human hepatocytes to affect expression of major P-450 genes and that a wide range of responses can be observed among the enzymes for a given cytokine, suggesting that different regulatory mechanisms may be involved.