Showing papers in "Experimental Parasitology in 1988"

TL;DR: A genetically homogeneous population of Plasmodium falciparum prepared by a single erythrocyte micromanipulation technique was used to produce lines of P. falcIParum resistant to mefloquine hydrochloride in vitro.

TL;DR: A rapid and definitive method of cloning was developed using micropipets and a micromanipulator to obtain stable strains of the parasites for long term experiments, and homogeneous parasite clones prepared by this technique were characterized and compared with the parent isolates.

TL;DR: The results strongly suggest that guanine nucleotide binding proteins are involved in T. cruzi adenylate cyclase activation and may be useful for studying cell differentiation mechanisms in eukaryotes.

TL;DR: The binding of infected red blood cells (IRBCs) to melanoma cells was specific to cells containing mature parasites and was not related to the level of peripheral parasitemia of the isolate or to differences in adaptation to culture conditions.

TL;DR: In pigs naturally exposed to eggs on a contaminated dirt lot, pigs became almost totally immune to second-stage larvae migrating from the intestines because few larvae from a challenge inoculum could be found in the lungs, and liver white-spot lesions were absent.

TL;DR: The sequence of an mRNA encoding superoxide dismutase (EC 1.15.1.1) of Schistosoma mansoni has been determined and the deduced amino acid sequence shows 40 to 45% homology with the amino acid sequences of the cytosolic superoxide Dismutase from 10 other species, as well as similar homologies with the human extracellular form.

TL;DR: The conservation of overall secondary structure shown by the hydropathy profile comparison indicates that gp195 proteins of the various P. falciparum isolates are functionally equivalent, supporting the proposed allelic dimorphism of gp195 within the parasite population.

TL;DR: These experiments establish that the explusion of N. brasiliensis from the intestine of the rat coincides not only with quantitative, but also with remarkable qualitative changes in the histochemical composition of mucins in goblet cells.

TL;DR: The results of these studies strongly suggest that division of E. histolytica involved a pleuromitotic process which is carried out in about 120 min.

TL;DR: A strong positive correlation between the percentage of reticulocytes and in vitro invasion was demonstrated, and a preferential invasion into reticULocytes was demonstrated in vivo and in vivo using blood films stained with brilliant cresyl blue and counterstained with Giemsa.

TL;DR: Biological/functional changes in promastigotes of Leishmania braziliensis panamensis may be related to an involvement of a heat shock response in the differentiation of leishmania, thus having important implications in the development of prevention and treatment stratagems.

TL;DR: Evidence is presented indicating that there are at least two "Portland 1" strains being used by the current scientific community, one containing the virus and the other lacking the virus.

TL;DR: Genes encoding antigens of Eimeria acervulina were cloned from cDNA expression libraries prepared from the sporozoite and merozoite stages in order to examine humoral and cellular immune responses to this protozoan parasite.

TL;DR: Differential and isopycnic centrifugation of homogenates demonstrated that giardial hydrolases were localized in a single-particle population of lysosome-like organelles suggesting tight binding to the organelle membrane.

TL;DR: The results indicate that P. carinii trophic and cyst forms have surface constituents containing mannose, N-acetylglucosamine and N- acetylgalactosamine as the predominant carbohydrates.

TL;DR: Comparison of the T. cruzi populations before and after feeding demonstrates that the trypomastigotes in the urine should originate from the rectal wall, especially from the posterior part.

TL;DR: The molecular characterization of the different cell forms by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of metabolic pulse labeled proteins showed that the in vitro differentiated cells were distinct, not only by morphological criteria, but by differential gene expression as well.

TL;DR: The molecular weight by P2 chromatography showed [125Sb]Pentostam to be of multiple species of MW = 100-4000 Da, rather than the one species of 746 Da predicted by the commonly hypothesized structural formula, which indicates that the multiple components of Pentostam (Sb and derivatives of gluconic acid) are more closely complexed with each other than previously thought.

TL;DR: It is suggested that virulence does not necessarily correlate with a pathogenic zymodeme, and the pathogenic potential of E. histolytica varies between isolates and can be affected by culture conditions and by the presence or absence of bacterial cells.

TL;DR: A characteristic lipid composition is found in cercariae, schistosomula, and adults and was similar but not identical to that of the snail host.

TL;DR: In surviving, paired females, irrespective of the initial severity of the drug-induced damage, both the vitelline gland and the ovary completely redeveloped and lead eventually to a resumption of egg production, and in unpaired, previously mature females the reproductive system also regressed but did not redevelop.

TL;DR: The expression of the SMw32 proteinase in developing schistosomula has been correlated with digestive tract development and a dramatic rise in enzyme activity as early as Days 8-10 of culture, and cysteine proteinase activity is essential in providing nutrients for the growth and survival of this parasite in its mammalian host.

TL;DR: The results probably indicate that only a low number of receptors for CaFl is still present in glands of infected snails and indicate that schistosomin and CaFl interact at the level of receptor complex(es) in the gland.

TL;DR: Oocysts in mosquitoes that had been fed anti-sporozoite antibodies on Day 5 p.i. produced significantly more sporozoites than did oocystic cells in nonimmune-fed mosquitoes, and the sporozoitic cells from Day 5 immune- fed mosquitoes were significantly more infective to cultured human hepatoma cells than were sporozoite cells from non immune-fed controls.

TL;DR: The dose-response relationship of schistosomin shows that at a 1:2 dilution of Sinf with Ringer the response to CaFl was reduced more than 50%.

TL;DR: The results suggest that the strains of chickens having improved protection against challenge infection demonstrate higher T-cell responses to the recombinant surface merozoite protein, p150.

TL;DR: Culture supernatants of metabolically labeled Leishmania donovani promastigotes were shown to contain approximately 40 electrophoretically distinct released protein compounds, which contained terminal mannose residues, as judged by their specific binding to concanavalin A-agarose beads.

TL;DR: The authors' data indicate substantial biochemical differences exist between morphologically similar organisms, and experiments using any type of artificially transformed schistosomule must be interpreted with caution until additional biochemical and physiological studies on cercarial transformation are undertaken.

TL;DR: It was demonstrated that the Plasmodium falciparum protein is synthesized at the trophozoite stage, accumulates at the schizont stage, and is processed at the merozoite stage to a triplet of three polypeptides.