Showing papers in "FEBS Letters in 1981"

TL;DR: The properties of oxygenevolving PS II preparations obtained by detergent resolution of chloroplast thylakoid membranes are reported.

TL;DR: The role of this radical @ iron-dependent damage to the sugar deoxyribose is examined, showing that treatment of several amino acids or carbohydrates with iron (II) salts in phosphate buffer causes formation of a product that reacts with thiobarbituric acid (TBA) to produce a chromogen.

TL;DR: In this article, the results of an investigation of acid and some similar forms of bovine and human a-lactalbumins are presented, which show that these forms are compact, have secondary structure similar to the native one but their tertiary structure can slowly fluctuate.

TL;DR: Purification of soluble guanylate cyclase from bovine lung can be achieved without major loss in stimulation by sodium nitropmsside and related agents, and a chromophore is a heine, which has been identified as ferroprotoporphyrin IX and copper was detected in the purified preparations.

TL;DR: The formation of a novel dihydroxy-acid, 5S,12S-dihydroxy-6-trans,8-cis,lOtrans,l4-c is-eicosatetraenoic acid (SS,12SDHETE), in preparations of human leukocytes is reported and further conversion of this trihydroxy acid to a dicarboxylic acid (20-COOH-LTB4).

TL;DR: A new experimental procedure is developed for detecting msCyt in CpG sequences, which is found to be highly methylated in animal cells and may be the major determinant in the placement of methyl groups on DNA.

TL;DR: The quantitative excretion of HBBL and HBBD was found to exhibit a cyclic pattern during the menstrual cycle of humans and monkeys, maximum excretion occurring in the luteal phase and elevated levels were found in urine collected during early pregnancy.

TL;DR: The thiobarbituric acid reaction has been widely used during the last 40 years for detecting oxidative deterioration in bulk lipids, and more recently it has been increasingly applied to complex biological materials, leading to widespread belief that the TBA test detects MDA formation in biological systems.

TL;DR: In this article, it was shown that the binding of PQ and of inhibitor to PS II centers with vacant secondary PQ binding sites (PS II), are competitive reactions, implying that its binding site is frequently left vacant.

TL;DR: The results suggest that the Limulus amebocytes contain two independent coagulation pathways, endotoxin-mediated and (1 + 3)O-D-glucan-mediated pathways, both of which result in the transformation of coagulogen to coagulin.

TL;DR: A highly active Oz-evolving PS-2 preparation from spinach chloroplasts was isolated using a low concentration of digitonin and Triton X-100 and the result was compared with that obtained by Tris treatment of unfractionated broken cloroplasts.

TL;DR: The inhibitory action and binding characteristics of the new anti-fungal antibiotic myxothiazol has been described and a red shift of the ferrocytochrome b spectrum is indicated, independent of and differs from the antimycin red shift.

TL;DR: Fractionation of ~ylakoids into grana and stroma thylakoid fractions by detergent or mechanical methods shows that the large subchlo-fracture particles located in appressed and exposed membranes reflect a difference in the distribution of the main intrinsic macromolecular complexes of thylAKoid membranes in the two regions.

TL;DR: Data is reported concerning the relative contribution of these two enzymes in maintaining the ascorbic acid system in the reduced state.

TL;DR: Here it is shown that S. typhimurium mutants (pmrA mutants) represent a novel type of mutation affecting the lipid A structure: the esterlinked phosphate in their lipid A is almost completely substituted with 4-ARAN, and their LPS has also an increased content of ethanolamine.

TL;DR: The quantitative aspects of the protein blotting technique are studied in order to estimate the amounts of individual complex III subunits in cell lysates and it is reported that under the authors' conditions the amounts are higher than expected.

TL;DR: It is concluded that hydrogenase from methanogenic bacteria is a nickel protein, and the synthesis of active hydrogenase in Methanobacterium thermoautotrophicum is dependent on nickel.

TL;DR: It is established that cytochrome c oxidase pumps protons in addition to the formation of a membrane potential and concomitan{ alkalinisation of the matrix side, due to the transfer of electrons from cy tochrome c to oxygen.

TL;DR: Key links are established between the redox state of plastoquinone, the activation of protein kinase and changes in chlorophyll fluorescence and the relative rates of excitation of PSI1 and PSI is presented.

TL;DR: CD and fluorescence were used to study the interaction of the model peptide melittin with dimyristoylphosphatidylcholine bilayer liposomes and found tryptophan residue is buried deeper within the membrane and is in a more hydrophobic microenvironment.

TL;DR: The role of LTB4, SQ,12(S)-DHETE and their o-oxida- tion products in leukocyte degranulation in vitro is investigated.

TL;DR: The present determination confirms that the GIP preparation is heterogeneous, and shows that the major secondary component appears to be identical to the most abundant component except that it lacks the first two residues of the latter.

TL;DR: In these studies, it is found that glucose is covalently bound to e-amino groups of lysine of human apo-lipoproteins upon incubation, in vitro and the level of glycosylated apoprotein B (apo-B) of the low density lipoprotein (LDL) is increased in the serum from diabetic patients.

TL;DR: The formation of 5S,12Sdihydroxy_6,8,10,14eicosatetraenoic acid and5S, 12S,20-trihydroxy-6, 8, 10,14- eicos atetraenosic acid in preparations of human leukocytes is described.

TL;DR: It is reported that Na* in the medium was required for the swimming of two independently isolated alkalophilic Bacillus, which suggests that the flagellar motors of these bacteria are powered by the transmembrane electrochemical potential gradient of Na+, ApNa+.

TL;DR: Several attempts have been made to localize different components across the thylakoid membrane and it has been revealed that components like ferredoxin, ferred toxin-NADP-reductase, the coupling factor and the light-harvesting complex ‘are all accessible from the outside.