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Showing papers in "Journal of Bioscience and Bioengineering in 2023"


Journal ArticleDOI
TL;DR: In this paper , local metabolic response in multilevel regulated and multibranched biosynthetic pathway (MRMBP) was investigated and various crucial genes in branched pathways were determined.

3 citations


Journal ArticleDOI
TL;DR: In this paper , an amperometric lactate biosensors based on lactate oxidase immobilization (with Nafion 5% wt) were designed and the limit of detection, linear range, and sensitivity values were determined to be 31 μM, 50-350 μM and 0.04 μA μM−1 cm−2, respectively.

2 citations


Journal ArticleDOI
TL;DR: In this paper , the authors used Aloe vera extract in different concentrations to synthesize hybrid nanoflowers (AV-Nfs), which showed antimicrobial activity against Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae at minimum inhibitory concentration of 625 μg/mL.

2 citations


Journal ArticleDOI
TL;DR: In this paper , the impact of human endometrial stem cells (EnSCs) and titanium oxide nanoparticles (TiO2 NPs) on dental pulp repair and regeneration in an animal model through dentine development and tissue regeneration.

2 citations


Journal ArticleDOI
TL;DR: In this paper , an endo-lytic enzyme that releases nigero-oligosaccharides (tetra-, tri-, and di-saccharide) from α-1,3-linked oligomers of glucose was investigated.

2 citations


Journal ArticleDOI
TL;DR: A recent review as discussed by the authors summarizes the current technologies for enhancing production, recovery, stability, and appreciable use of bacterial pigments in industries apart from therapeutics with proper financial aspects.

1 citations


Journal ArticleDOI
TL;DR: In this article , the effects of Jiang-flavor baijiu on organ function and gut microbiota were investigated in a moderate drinking mouse model and the results showed that moderate dose Jiang-FLAVOR consumption increased the abundance of beneficial taxa such as Lactobacillus and Akkermansia and increased the harmful bacteria such as Prevotella and Mucispirillum.

1 citations



Journal ArticleDOI
TL;DR: In this paper , the authors explored the use of bacteriophage-derived molecules as probes for detecting and isolating uncultured bacteria by using multiplex single-cell sequencing and searching prophage sequences from over 450 obtained human oral bacterial single-amplified genomes.

1 citations


Journal ArticleDOI
TL;DR: In this paper , the authors investigated changes in the functional components, such as free amino acids and polyphenols, of the respective obtained Moringa tempe Rm and Rs.

1 citations


Journal ArticleDOI
TL;DR: In this paper , a gram-positive bacterium, Corynebacterium glutamicum, with a high tolerance to fermentation inhibitors was metabolically engineered to express a fusion protein composed of cis-aconitate decarboxylase from A. terreus and a maltose-binding protein (malE) from Escherichia coli.

Journal ArticleDOI
TL;DR: In this article , the authors analyzed fungal communities and populations during laboratory-scale spontaneous winemaking using sterilized labware to avoid winery-resident microbes and found that S. cerevisiae was eventually dominant in seven of 12 fermentation batches.

Journal ArticleDOI
TL;DR: Lactate is an important biomarker for fat oxidation in skeletal muscles as discussed by the authors , and it is used to confirm exercise performance and should be further studied to assess its impact on exercise training.

Journal ArticleDOI
TL;DR: In this article , the co-production of allitol and gluconic acid (GA) was investigated in Escherichia coli, where the recombinant strain Ec/pAd-pRrg boosted NADH production to 2.4 μmol/gDCW, 118% higher than with the control strain.

Journal ArticleDOI
TL;DR: In this paper , the authors developed an Artificial Intelligence (AI) model to predict ethanol yields in yeast fermentation cultures under conditions of high-sugar stress using cell morphological data.

Journal ArticleDOI
TL;DR: In this paper , a combination of cold water extract (LAE) and Thymus vulgaris essential oil (TO) was found to be highly effective against the C. annuum pathogens.

Journal ArticleDOI
TL;DR: In this article , the authors evaluated the properties of enzyme-modified cheese with added protease (Ep) or lipase (El), including texture, sensory, organic acids, volatile compounds, and free amino acids.

Journal ArticleDOI
Hiroaki Iwaki1
TL;DR: In this article , the authors identified two non-contiguous genetic loci spanning 22 kb of Paraburkholderia (formerly Burkholderia) sp. strain KU-46.

Journal ArticleDOI
TL;DR: In this paper , the authors have characterized phylogenetic compositions and diversity of the bacterial community in a sake brewery performing the Kimoto-style fermentation and found a novel type of Kimoto fermentation in which the microbial transition differed significantly from other breweries during the fermentation step.

Journal ArticleDOI
TL;DR: In this article , a trans-pathway is used for IA production in engineered Corynebacterium glutamicum ATCC 13032 pCH-Tad1optadi1opt, which achieved high titers of 12.25, 11.34, and 11.02 g/L.

Journal ArticleDOI
TL;DR: In this paper , the authors developed an efficient biocatalytic system to produce formate selectively by coupling two enzymatic activities of H2 oxidation and CO2 reduction using encapsulated bacterial cells of Citrobacter sp. S-77.

Journal ArticleDOI
TL;DR: In this paper , an enzymatic colorimetric method was developed for the determination of l-lactic acid by competitive indicator displacement assay, which achieved turn-on and visual sensing with good precision, accuracy, specificity, and robustness.

Journal ArticleDOI
TL;DR: In this article , the authors evaluated the gut mycobiota of ten Japanese infants during the first 3 years of life and found that the most abundant genus was Saccharomyces (26.8%), followed by Malassezia (18.5%), Candida (12.3%), Meyerozyma (8.5%).

Journal ArticleDOI
TL;DR: In this article , the authors assessed the risk of horizontal gene transfer driven escape using the Pt-dependent cyanobacterium Synechococcus elongatus PCC 7942 and showed that removing a single gene necessary for natural competence from the Ptdependent strain reduced the escape occurrence rate.


Journal ArticleDOI
TL;DR: In this article , Ferrous-oxidizing bacteria (FOB) and sulfuroxidising bacteria (SOB) were mass-cultivated in a hybrid bioreactor equipped with an internal circulation system.

Journal ArticleDOI
TL;DR: In this article , the authors proposed a new approach to expand Chinese hamster ovary (CHO) cells using cell-sized condensed and crosslinked gelatin microparticles (GMPs) as carriers.

Journal ArticleDOI
TL;DR: In this article , the authors hypothesized that overproduction of one of the endogenous isoprenoids, in addition to isopentenyl diphosphate itself, might be the cause of the reported reduced growth rate and attempted to identify the causative agent.

Journal ArticleDOI
TL;DR: In this article , the authors presented a hybrid approach that combines random displacement amplification sequencing (RamDA-seq) with Cas9-based rRNA depletion for scRNA-seq in bacteria.

Journal ArticleDOI
TL;DR: In this article , a commercial Ganoderma extract was partially separated into three fractions by preparative high-performance liquid chromatography, and the separated fractions were then directly biotransformed by a Bacillus glycosyltransferase (BsUGT489).