Showing papers in "Journal of Pharmaceutical and Biomedical Analysis in 2003"

TL;DR: A method for the determination of the degree of deacetylation of chitosan by 1H NMR spectroscopy has been formally validated and is found to be simple, rapid and more precise than other known techniques like IR or titration for %DDA measurements.

TL;DR: A liquid chromatography/mass spectrometric method was developed and will be a useful tool in the investigation of the roles of these important thiol-containing compounds and their corresponding disulfides in physiological and pathological processes.

TL;DR: There was relatively little variability between subjects and study days, and in the case of endogenous urinary metabolite profiles there was considerable inter-subject variability, but less intra-subject variation, which suggests the possibility to collect consistent metabonomics data in clinical studies.

TL;DR: The CZE and MEKC methods are suitable for the qualitative and quantitative determination of combined HCT/ARA-IIs in pharmaceutical formulations and have been validated in terms of their linearity of response, reproducibility, and accuracy.

TL;DR: A simple and sensitive method for separation and determination of harmol, harmalol, riskine and harmaline in the seeds of Peganum harmala L has been developed and validated.

TL;DR: In the 'test of acceptability', the use of the interval hypotheses is envisaged by the SFSTP guide, not on the parameters of bias and precision, but on individual results by mixing mean bias and intermediate precision in a single test.

TL;DR: This review covers the time period from 1995 to 2001 during which around 450 analytical methods including all types of chromatographic and hyphenated techniques were reported and HPLC with UV detection was found to be the technique of choice for many workers and more than 200 methods were developed using LC-UV alone.

TL;DR: A simple high-performance liquid chromatographic (HPLC) method was developed for the analysis of atorvastatin and its impurities in bulk drug and tablets and has shown good resolution for AT, desfluoro-atorvastsatin (DFAT), diastereomer-ator vastatin (DSAT), unknown impurities and formulation excipients of tablets.

TL;DR: A sensitive column-switching HPLC method with fluorescence detection was developed for the determination of bisphenol A (BPA) in human blood serum and ascitic fluid samples and its relationships were observed between maternal and umbilical cord blood serum samples and patients with sterility.

TL;DR: Based on the dissolution rate, variance of flavonoid release and ascorbic acid equivalents, it was concluded, that Tinctura solidaginis (70% v/v ethanol) and Infusumsolidaginis are the most appropriate preparations.

TL;DR: A rapid, simple and sensitive ion-pair HPLC method has been developed for quantification of metformin in plasma and the coefficients of variation for inter-day and intra-day assay were within the range of clinical usefulness.

TL;DR: A reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous determination of diosmin, hesperidin and naringin in different citrus fruit juices and pharmaceutical preparations and has been successfully applied to the determination of all three flavonoid glycosides.

TL;DR: A method has been developed for the high-throughput inhibition screening of the major human cytochrome P450 (CYP) enzymes using an in vitro substrate cocktail and liquid chromatography-tandem mass spectrometry (LC-MS-MS).

TL;DR: The spectroscopy measurement showed an increase in the double bond amount with increasing enzymatic digestion time, and this double bond forms a complex with the radicals during UV irradiation, and reduced the toxicity of the radicals before they are absorbed in the human skin.

TL;DR: This review provides an overview as well as a practical understanding of on-line sample concentration techniques in micellar electrokinetic chromatography, known to be effective techniques for enhancement of the concentration sensitivity in MEKC.

TL;DR: The results show that this method has good correlation for DNA detection in the range of 10-800 pmol/l and allows the detection level as low as 5 pmol /l of the target oligonucleotides.

TL;DR: The main components of Orthosiphon stamineus Benth leaves and extracts are the pharmacologically active polyphenols: the polymethoxylated flavonoids and the caffeic acid derivatives.

TL;DR: The method was validated for the quantitation of PEI in the presence of an oligonucleotide, which absorbs at 285 nm as well and the detection limit (QOD) was 4.0 microg ml(-1).

TL;DR: A flow injection method is proposed for the determination of paracetamol in pharmaceutical dosage forms and the results were compared with those obtained by the official HPLC method, with relative standard deviations below 1%.

TL;DR: The application of electrochemical methods on the interactions between DNA and DNA targeted agent were explored and the detection limit and the reproducibility was determined by using both electrochemical transducers.

TL;DR: High performance liquid chromatography and second derivative spectrophotometry have been used and described for the simultaneous determination of montelukast and loratadine in pharmaceutical formulations and results confirm that the methods are highly suitable for its intended purpose.

TL;DR: A simple, rapid and accurate high-performance liquid chromatography method was developed for the assessment of three bioactive triterpene acids: tormentic acid (TA), oleanolic acid (OA) and ursolic Acid (UA) in Perilla frutescens.

TL;DR: In CE, the advantages of having extremely low mass detection limits and requiring only a very short analysis time are made possible by using laser-induced fluorescence or near infra-red detections.

TL;DR: A simple and rapid isocratic LC/MS coupled with electrospray ionization (ESI) method for simultaneous separation and determination of adenine, hypoxanthine, adenosine and cordycepin in Cordyceps sinensis and its substitutes was developed.

TL;DR: The validated method was successfully applied to simultaneous determination of enantiomers concentrations of CIT and DCIT in plasma samples from nine patients treated with racemic citalopram, confirming LPME-CE as a suitable and promising tool for enantiomeric determination of chiral drugs and metabolites in biological matrices.

TL;DR: A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of pioglitazone (PIO) and its two metabolites: M-III and M-IV in human plasma, and has been applied successfully to sample analysis for clinical studies.

TL;DR: An HPLC/MS/MS method was developed for the analysis of 'SHENMAI' injection, composed of red ginseng and ophiopogon and 39 ginsenosides were detected.

TL;DR: Processing of plasma samples under a 10% CO2 atmosphere is a method of choice for stability or protein binding studies in plasma, whereas citrate or phosphate buffers are suitable for stabilizing pH in bile and urine and for plasma samples requiring extensive preparations or long term storage.

TL;DR: A sensitive, simple and accurate method was developed and applied to determine quercetin and kaempferol after orally administrated tablet of Ginkgo biloba extract in human urine by reversed phase high performance liquid chromatography.

TL;DR: Zero-crossing method in the first-order derivative spectrophotometry showed the impurity-drug intermolecular interactions, confirmed by divergences of experimentally obtained amplitudes for impurities OMS and NPA in comparison to expected values according to regression equations of calibration graphs.