Showing papers in "Journal of Pharmaceutical and Biomedical Analysis in 2005"

TL;DR: A validated protocol for quantitative high resolution 1H-NMR using single pulse excitation that considers all issues regarding linearity, robustness, specificity, selectivity and accuracy as well as influences of instrument specific parameters and the data processing and evaluation routines is described.

TL;DR: Electrochemical approach can provide new insight into rational drug design and would lead to further understanding of the interaction mechanism between anticancer drugs and DNA.

TL;DR: This review gives an overview of the application of quantitative NMR spectroscopy in International Pharmacopoeias and for licensing purposes.

TL;DR: Chemical fingerprint combined with metabolic fingerprint is a useful means to control the quality and to clarify the possible mechanism of action of herbal products.

TL;DR: The development of international guidance on registration of technical dossiers supporting applications to market medicinal products and aspects of guidance of particular relevance to the use of NMR spectroscopy in drug development and control are discussed.

TL;DR: The solubility and dissolution rates of AMP were significantly increased by solid dispersions and cyclodextrin complexes as well as their physical mixtures.

TL;DR: Improved antioxidant efficacy of rutin was investigated, as affected by molecular encapsulation within beta-cyclodextrin (batophenanthroline test; comet assay; lipid peroxidation); the inclusion complex revealed improved antioxidant efficacy that may be in part explained by an increased solubility in the biological moiety.

TL;DR: A simple, selective, precise and stability-indicating high-performance thin-layer chromatographic method of analysis of curcumin both as a bulk drug and in formulations was developed and validated.

TL;DR: A new method involving SA as fluorescence-enhancing reagent for estimation of BRD in aqueous samples has been suggested, and selective excitation of tryptophan residue results in emission from bromadiolone, thereby indicating a Förster type energy transfer from Trp to BRD.

TL;DR: It is the authors' conclusion that strategies such as chemical derivatization and on-line H/D exchange should be used more routinely in drug metabolism studies in order to facilitate metabolite identification.

TL;DR: Overall, compatibility of excipients with glipizide was successfully evaluated using the combination of thermal and IST methods and the formulations developed using the compatibleexcipients was found to be stable.

TL;DR: According to the life-cycle concept of validation, measures should be taken to maintain and control the validated status of analytical procedures during long-term routine application, such as monitoring relevant performance parameters (system suitability tests), control charts, etc.

TL;DR: This multiplex immunoassay provided simultaneous measurement of circulating cytokines using 80% less patient specimen compared to traditional approaches and at a significantly decreased cost.

TL;DR: A prototype commercial instrument and 2.1 mm i.d. columns packed with 1.7 microm porous particles have been used to measure peak capacity in ultra performance liquid chromatography (UPLC) as mentioned in this paper.

TL;DR: The capillary electrophoresis method for the determination of rosemary active components using electrochemical detection was successfully used with relatively simple extraction procedures, and the assay results were satisfactory.

TL;DR: The developed method was successfully applied to estimate the amount of ezetimibe in tablets and was found to be linear from 0.5 to 50 microg/ml.

TL;DR: In this paper, a stability indicating reversed-phase HPLC method has been developed and subsequently validated for simultaneous estimation of amlodipine (AM) present as AM besylate (AB), and benazepril hydrochloride (BH) from their combination product.

TL;DR: It was determined that although mixed mode phases fulfills the requirements of retaining both analytes and diverse metabolites, reverse phase retention mechanisms are detrimental in eliminating ion suppression caused by late eluting phospholipids.

TL;DR: The results suggest that Glycyrrhiza glabra and Ginkgo biloba extracts may be used in topical formulations in order to protect skin against damage caused by free radical and reactive oxygen species.

TL;DR: It was found that quantitative Raman assessment of chemical content can be made insensitive to variations in tablet density corresponding to a manufacturing compression interval of 5-20 kN provided that adequate data treatment is used.

TL;DR: Application of the variable ranking method to the models showed high importances for the n-octanol/water partition coefficient (logP) and polar surface area (PSA) which shows that CART is capable of selecting the most important descriptors for the absorption process in the intestinal tract.

TL;DR: The results of synchronous fluorescence spectra, UV-vis absorption spectra and circular dichroism of BSA in presence of fluoroquinolones show that the conformation of bovine serum albumin changed.

TL;DR: The antioxidant activity of extracts of propolis and of formulations added with these extracts were measured by scavenging different radicals in different systems using both lipid peroxidation and chemiluminescence generated in the xanthine/luminol/XOD system inhibitions.

TL;DR: A direct, extraction-free spectrophotometric assay was developed and shown to be direct, sensitive, accurate, reproducible, and less expensive without separation of HA from ACE reaction mixture, and can be used for the screening of ACE inhibitory peptides derived from food proteins.

TL;DR: In this paper, the authors focused on the characterization of inclusion complexes of BZC in β-cyclodextrin (β-CD) and showed that a three-fold increase in BZc solubility can be achieved upon complexation with β-CD.

TL;DR: The results indicated that the developed HPLC assay can be readily utilized as a quality control method for P. notoginseng.

TL;DR: Both analytical techniques used in this study were able to detect differences between normal and Zucker obese rats, which may provide markers of metabolic disease.

TL;DR: An assay based on cation exchange solid-phase extraction and liquid chromatography-tandem mass spectrometry (LC/MS/MS) has been developed and exhibited high selectivity, since no effect on metformin analysis was observed on comparison of samples with or without nateglinide and other agents in plasma.

TL;DR: A simple reversed-phase high performance liquid chromatographic method with UV detection at 270 nm was developed for simultaneous quantitation of ketoprofen and naproxen sodium along with phenol red as a non-absorbable marker for in situ permeability studies.

TL;DR: This paper compares the performance of two recently developed algorithms and methods for peak alignment of first-order NMR data of complex biological samples and shows that both alignment methods have been shown to enhance the interpretability of the resulting multivariate models, thereby increasing the prospect of detecting and following the onset of subtle biological changes reflected in the N MR data.