Showing papers in "Journal of Pharmaceutical and Biomedical Analysis in 2012"

TL;DR: Monitoring, by NIR, of many manufacturing processes, such as granulation, mixing or drying, in order to determine the end-point of these processes, and determinations of the quality and quantity of pharmaceutical compounds are included.

TL;DR: Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents as mentioned in this paper, which is based on the use of a biologically related agent as a stationary phase to selectively retain analytes or to study biological interactions.

TL;DR: This write-up critically reviews the literature for application of hyphenated tools in impurity and degradation product profiling of small molecules and appropriate strategies are proposed, following which one can obtain unambiguous characterization of the unidentified IMPs and/or DPs.

TL;DR: In this review a practical overview is given of the different parameters that are important in supercritical fluid chromatographic separations and a comparison is made between the applicability for chiral separations of SFC and conventional high-performance liquid chromatography (HPLC), and illustrated by means of examples.

TL;DR: The scale at which erectile dysfunction (ED) medicines are obtained outside of the official health system rivals and possibly exceeds legitimate sales and according to literature a high-risk segment of this market is occupied by adulterated food supplements.

TL;DR: An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS)-based metabolomics approach was developed to evaluate the holistic qualities and to explore characteristic chemical components of commercial WG and RG.

TL;DR: The present review focuses on the basics of RPLC for peptides and proteins, including the inherent problems, some possible solutions and some directions for developing a new RPLC method that is dedicated to biomolecules.

TL;DR: Molecular dynamic simulation revealed that HSA does not have a significant conformational change when it binds with harmalol, and excellent agreement was found between the experimental and theoretical results with respect to the mechanism of binding and binding constants.

TL;DR: Bioanalytical procedures for the determination of markers of ethanol consumption during pregnancy and related prenatal exposure are reviewed and clinical toxicological applications of these procedures are presented and discussed.

TL;DR: The results clearly showed that quality by design concept could be effectively applied to optimize an HPLC chromatographic method for protein analysis with the least number of experimental runs possible.

TL;DR: In this review, the literature is surveyed for developed and validated HPTLC methods to assay active ingredients in pharmaceutical formulations published in the period 2005-2011.

TL;DR: This review gives a comprehensive and critical overview of the methodologies applied to assess the validity of quantitative NIRS methods used in pharmaceutical applications.

TL;DR: This review surveys indirect and direct HPLC separations of biologically and pharmaceutically important enantiomers of amino acids and related compounds, with emphasis on the literature published from 2005.

TL;DR: The validated LC-MS/MS method was successfully applied to investigate the pharmacokinetics of the major flavonoids of Radix scutellariae extract after oral administration to rats.

TL;DR: The present study suggests that the age of ginseng could be successfully predicted using two solvents, and the developed method can be used as a standard protocol for discriminating and predicting the ages of gINSeng root samples.

TL;DR: An overview of the key performance characteristics and manifold applications for HPLC-CAD in the field of pharmaceutical analysis is provided.

TL;DR: It was observed that specifically positive adsorbent (silica gel) strengthened the observed result of radical-antioxidant reaction, while polar bonded stationary phase CN-silica weakened it.

TL;DR: The D-amino acid research is described, from the discovery of appreciable amounts of free D-Amino acids in mammals to the current metabolomics study focusing on amino acid enantiomers.

TL;DR: The same MBDA score was observed in different subjects despite having different biomarker profiles, supporting prior literature reports that multiple pathways contribute to RA.

TL;DR: An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry method coupled with a principal component analysis (PCA) has been developed and successfully applied to the identification of variants of gelatins commonly used in Traditional Chinese Medicine (TCM).

TL;DR: Mechanism of interaction of bioactive flavonoids, hesperitin (HES) and naringenin (NAR) with calf thymus deoxyribonucleic acid (DNA) was studied employing UV absorption, fluorescence, circular dichroism, melting temperature,fluorescence anisotropy and differential pulse voltammetric methods.

TL;DR: The overall results sufficiently demonstrate that the spectroscopy coupled with the SVMR regression tool has the potential to measure AA in green tea.

TL;DR: Major benefits of LC-MS/MS are the improved selectivity, precision and accuracy, the higher sensitivity and the unaltered capacity of detection with time in contrast to ECD.

TL;DR: It is found that SZRD increases sleep activity and exhibits binding affinity for serotonin receptors, which implicate the therapeutic effects of SzRD may mediate through serotonergic activation and show the robust UPLC/ESI-SYNAPT-HDMS techniques is promising for metabolites profiling analysis of TCM and open new perspectives to using metabolomics platform to resolve special TCM issues.

TL;DR: The metabolites profiles in plasma revealed that glucuronide conjugates of isoflavonoids and flavonoid aglycones were the major circulating forms of icariin.

TL;DR: It is proposed that a traditional aqueous preparation from Artemisia annua, with a low content of the active compound, artemisinin, may reduce the risk of resistance of the protozoa and be relatively more effective in the treatment of the disease.

TL;DR: Citalopram, loperamide, methadone, paroxetine, pethidine, and sertraline were extracted exhaustively with electromembrane extraction (EME) by increasing the number of hollow fibers from one to three to demonstrate the importance of an electrical field.

TL;DR: The cocrystal formation of indomethacin (IMC) and saccharin (SAC) by mechanical cogrinding or thermal treatment was investigated and the main IR spectral shifts suggested that the OH and NH groups in both chemical structures were taken part in a hydrogen bonding, leading to the formation of IMC-SAC cocystal.

TL;DR: The developed method proved to be suitable for routine work and it was used to successfully analyze more than 2500 clinical and forensic blood samples.

TL;DR: The method showed good system suitability, sensitivity, linearity, specificity, precision, stability and accuracy, and was reliable and convenient for the rapid determination of VB (1), VB(3),VB(6), VC, VB-5, Vb(2) and VB-(9) in VMT.