Book ChapterDOI
[52] Preparation of high-purity plasma membranes
TLDR
This chapter describes a method using the preparation of plasma membranes from light-grown oat leaves as an example, and estimates the purity to be higher than 90% and often close to 100%, plasma membrane and similar purities are reported for plasma membrane from maize roots.Abstract:
Publisher Summary This chapter describes a method using the preparation of plasma membranes from light-grown oat ( Arena sativa L. ) leaves as an example. Using the batch procedure, two fractions containing purified plasma membrane (U 3 and U 3 , ) and one fraction containing intracellular membranes depleted of plasma membrane (L 1 ) are obtained. Specific staining with phosphotungstic acid or silicotungstic acid seems to be the only universal marker for the plant plasma membrane and the only one that permits a real estimation of the purity of the preparations. Based on this staining the purity is estimated to be higher than 90% and often close to 100%, plasma membrane and similar purities are reported for plasma membrane from maize roots. (>90%) for plasma membrane preparations obtained both by phase partitioning and free flow electrophoresis.read more
Citations
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Journal ArticleDOI
A Plant Homolog of the Neutrophil NADPH Oxidase gp91phox Subunit Gene Encodes a Plasma Membrane Protein with Ca2+ Binding Motifs
Thomas Keller,Howard G. Damude,Dietrich Werner,Peter Doerner,Richard A. Dixon,Christopher J. Lamb +5 more
TL;DR: It is proposed that plants have a plasma membrane enzyme similar to the neutrophil NADPH oxidase but with novel potential regulatory mechanisms for Ca2+ and G protein stimulation of O2− and H2O2 production at the cell surface.
Journal ArticleDOI
Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber
Xiao-Jian Xia,Yan-Jie Wang,Yan-Hong Zhou,Yuan Tao,Wei-Hua Mao,Kai Shi,Tadao Asami,Zhixiang Chen,Jing-Quan Yu +8 more
TL;DR: Results strongly suggest that elevated H2O2 levels resulting from enhanced NADPH oxidase activity are involved in the BR-induced stress tolerance.
Journal ArticleDOI
Superoxide Production by Plant Homologues of the gp91phox NADPH Oxidase. Modulation of Activity by Calcium and by Tobacco Mosaic Virus Infection
Moshe Sagi,Robert Fluhr +1 more
TL;DR: In contrast to the mammalian gp91(phox), the plant homolog can produce O(2)(-) in the absence of additional cytosolic components and is stimulated directly by Ca(2+), indicating that the formazan precipitates were due to reduction by O( 2)(-) radicals catalyzed by an NADPH-dependent flavin containing enzyme.
Journal ArticleDOI
Direct ligand–receptor complex interaction controls Brassica self-incompatibility
Seiji Takayama,Hiroko Shimosato,Hiroshi Shiba,Miyuki Funato,Fang-Sik Che,Masao Watanabe,Megumi Iwano,Akira Isogai +7 more
TL;DR: It is shown that a single form of SP11 of the S8 haplotype (S8-SP11) stabilized with four intramolecular disulphide bonds specifically binds the stigma membrane of theS8 haplotypes to induce autophosphorylation of SRK8, and thatSRK8 and SLG8 together form a high-affinity receptor complex for S8- SP11 on the stigma membranes.
Journal ArticleDOI
Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings.
Mingyi Jiang,Jianhua Zhang +1 more
TL;DR: Data indicate that NADPH oxidase is involved in the ABA-induced production of active oxygen species (AOS), and results depict a minimal chain of events initiated by water stress-induced ABA accumulation, which then triggers the production of AOS by membrane-bound NAD PH oxidase, resulting in the induction of antioxidant defense systems against oxidative damage in plants.
References
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Journal ArticleDOI
Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay
TL;DR: A modification of two similar procedures further increases the sensitivity, simplicity, and stability of the protein-dye binding assay over those of other commonly used assays for protein.
Journal ArticleDOI
Effect of Vanadate, Molybdate, and Azide on Membrane-Associated ATPase and Soluble Phosphatase Activities of Corn Roots
TL;DR: While vanadate does not appear to be a selective inhibitor, it can be used in combination with molybdate and azide to distinguish the plasma membrane ATPase from mitochondrial ATPase or supernatant acid phosphatase.
Journal ArticleDOI
Preparation and polypeptide composition of chlorophyll‐free plasma membranes from leaves of light‐grown spinach and barley
Per Kjellbom,Christer Larsson +1 more
TL;DR: The polypeptide composition of the barley and spinach plasma membranes showed striking similarities, with the most prominentpolypeptides in the 49-58 kdalton region, and some further prominent bands in the 30 kcialton region.
Journal ArticleDOI
Sidedness of plant plasma membrane vesicles purified by partitioning in aqueous two‐phase systems
TL;DR: Preparations of plant plasma membrane vesicles were obtained by partition in dextran‐polyethylene glycol two‐phase systems by separating particles according to their surface properties, and an iso‐osmotic environment is maintained throughout.
Journal ArticleDOI
Plasma Membranes from Oats Prepared by Partition in an Aqueous Polymer Two-Phase System On the Use of Light-Induced Cytochrome b Reduction as a Marker for the Plasma Membrane
TL;DR: Presumptive plasma membrane fractions have been prepared from oat roots and shoots by partition of microsomal fractions in a dextran-polyethylene glycol two-phase system, and relatively pure plasma membranes were obtained by only two partition steps, and within 3 hours from homogenization of the material.
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