Journal ArticleDOI
Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings.
Mingyi Jiang,Jianhua Zhang +1 more
TLDR
Data indicate that NADPH oxidase is involved in the ABA-induced production of active oxygen species (AOS), and results depict a minimal chain of events initiated by water stress-induced ABA accumulation, which then triggers the production of AOS by membrane-bound NAD PH oxidase, resulting in the induction of antioxidant defense systems against oxidative damage in plants.Abstract:
The roles of the plasma-membrane (PM) NADPH oxidase in abscisic acid (ABA)- and water stress-induced antioxidant defense were investigated in leaves of maize ( Zea mays L.) seedlings. Treatment by exogenous ABA (100 micro M ABA) or osmotic stress (-0.7 MPa induced by polyethylene glycol) significantly increased the activity of the PM NADPH oxidase, the production of leaf O(2)(-), the activities of several antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase), and the contents of antioxidant metabolites (ascorbate and reduced glutathione). Pretreatment with three different inhibitors of NADPH oxidase (diphenylene iodonium, imidazole and pyridine) or an inhibitor of ABA biosynthesis (tungstate) reduced the increase in the activity of the PM NADPH oxidase and the production of leaf O(2)(-), and the capacity of antioxidant defense systems mediated by ABA. The inhibitory effects above caused by tungstate were reversed by exogenous ABA. These data indicate that NADPH oxidase is involved in the ABA-induced production of active oxygen species (AOS), and our results depict a minimal chain of events initiated by water stress-induced ABA accumulation, which then triggers the production of AOS by membrane-bound NADPH oxidase, resulting in the induction of antioxidant defense systems against oxidative damage in plants.read more
Citations
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Journal ArticleDOI
NADPH oxidase AtrbohD and AtrbohF genes function in ROS‐dependent ABA signaling in Arabidopsis
June M. Kwak,Izumi C. Mori,Zhen-Ming Pei,Nathalie Leonhardt,Miguel Angel Torres,Jeffery L. Dangl,Rachel E. Bloom,Sara Bodde,Jonathan D. G. Jones,Julian I. Schroeder +9 more
TL;DR: Identification of two partially redundant Arabidopsis guard cell‐expressed NADPH oxidase catalytic subunit genes, AtrbohD and atrbohF, are reported, providing direct molecular genetic and cell biological evidence that ROS are rate‐limiting second messengers in ABA signaling, and that the AtrbOHD and AtrabohF NADPH oxidationases function in guard cell ABA signal transduction.
Journal ArticleDOI
The role of potassium in alleviating detrimental effects of abiotic stresses in plants
TL;DR: In this article, it was shown that an increase in capacity of bean root cells to oxidize NADPH when exposed to K deficiency was up to 8-fold higher in plants with low K supply than in K-sufficient plants.
Journal ArticleDOI
Active oxygen species and antioxidants in seed biology
TL;DR: Active oxygen species, which occurs during seed desiccation, germination and ageing, may lead to oxidative stress and cellular damage, resulting in seed deterioration, but cells are endowed with detoxifying enzymes and antioxidant compounds that scavenge AOS and participate in seed survival.
Journal ArticleDOI
Redox Regulation: A Broadening Horizon
Bob B. Buchanan,Yves Balmer +1 more
TL;DR: Research in areas currently under exploration promises to provide a fuller understanding of the role redox plays in cellular processes, and to further the application of this knowledge to technology and medicine.
Journal ArticleDOI
Nitric oxide signalling in plants
TL;DR: The potential sources of endogenous NO are reviewed, the biological processes likely to be mediated by NO are outlined, and the downstream signalling processes by which NO exerts its cellular effects are discussed.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book ChapterDOI
Catalase in vitro
TL;DR: In this article, the catalytic activity of catalase has been investigated using ultraviolet (UV) spectrophotometry and Titrimetric methods, which is suitable for comparative studies for large series of measurements.
Journal ArticleDOI
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Yoshiyuki Nakano,Kozi Asada +1 more
TL;DR: Observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ASCorbate is regenerated from DHA by the system: photosystem I-*ferredoxin-*NADP^>glutathione and a preliminary characterization of the chloroplast peroxidase is given.
Journal ArticleDOI
Superoxide Dismutases: I. Occurrence in Higher Plants
TL;DR: Nine of the enzyme activities were eliminated with cyanide treatment suggesting that they may be cupro-zinc enzymes, whereas one was cyanide-resistant and may be a manganese enzyme.
Journal ArticleDOI
Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine
TL;DR: It is reported here that 2-vinylpyridine is a much better reagent for the derivitization of glutathione, and it is demonstrated that the total glutATHione concentration in mouse plasma is substantially higher than generally reported and that glutathion disulfide constitutes less than 30% of the totalglutathione present.
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