Journal ArticleDOI
A comparative proteomic analysis of tomato leaves in response to waterlogging stress.
Nagib Ahsan,Dong-Gi Lee,Sang Hoon Lee,Kyu Young Kang,Jeong Dong Bahk,Myung Suk Choi,In-Jung Lee,Jenny Renaut,Byung-Hyun Lee +8 more
TLDR
A number of novel proteins were differentially expressed or appeared only in the PEG-fractionated protein samples, indicating that PEG fractionation system can be used as a versatile protein fractionation technique in proteomic analysis to identify novel or low-abundant proteins from all kinds of plant species.Abstract:
A comparative proteomic approach has been adopted in combination with physiological and biochemical analysis of tomato leaves responding to waterlogging stress. Waterlogging resulted in increases of relative ion leakage, lipid peroxidation and in vivo H2O2 content, whereas the chlorophyll content was decreased. Histocytochemical investigations with 3,3'-diaminobenzidine to localize H2O2 and Evans blue to detect dead cells suggested that oxidative stress has a significant role to leaf senescence. Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the most abundant leaf protein, was successfully reduced from the samples by a fractionation method based on 15% polyethylene glycol (PEG). Elimination of Rubisco was further confirmed by Western blot analysis. To elucidate the temporal changes of the protein patterns in tomato leaves, the total soluble and the PEG-fractionated proteins were separated by two-dimensional electrophoresis (2-DE) and visualized by Coomassie Brilliant Blue staining. A total of 52 protein spots were differentially expressed, wherein 33 spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry or electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis. The identified proteins are involved in several processes, i.e. photosynthesis, disease resistance, stress and defense mechanisms, energy and metabolism and protein biosynthesis. Results from 2-DE analysis, combined with immunoblotting clearly showed that the fragments of Rubisco large subunit were significantly degraded. This could result from a higher production of reactive oxygen species in leaves under waterlogging stress. Furthermore, four differentially accumulated proteins were analyzed at the mRNA level, confirming the differential gene expression levels and revealing that transcription levels are not always concomitant to the translation level. A number of novel proteins were differentially expressed or appeared only in the PEG-fractionated protein samples, indicating that PEG fractionation system can be used as a versatile protein fractionation technique in proteomic analysis to identify novel or low-abundant proteins from all kinds of plant species.read more
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Journal ArticleDOI
Plant proteome changes under abiotic stress--contribution of proteomics studies to understanding plant stress response
TL;DR: In this review, proteomics studies dealing with plant response to a broad range of abiotic stress factors--cold, heat, drought, waterlogging, salinity, ozone treatment, hypoxia and anoxia, herbicide treatments, inadequate or excessive light conditions, disbalances in mineral nutrition, enhanced concentrations of heavy metals, radioactivity and mechanical wounding are discussed.
Journal ArticleDOI
Comparative proteomic study of arsenic‐induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress
Nagib Ahsan,Dong-Gi Lee,Iftekhar Alam,Pil Joo Kim,Jeung Joo Lee,Young-Ock Ahn,Sang-Soo Kwak,In-Jung Lee,Jeong Dong Bahk,Kyu Young Kang,Jenny Renaut,Setsuko Komatsu,Byung-Hyun Lee +12 more
TL;DR: Comparative proteomic analysis of rice roots during As stress suggests that SAMS, CS, GSTs, and GR presumably work synchronously wherein GSH plays a central role in protecting cells against As stress.
Journal ArticleDOI
Plant proteomics update (2007-2008) : Second-generation proteomic techniques, an appropriate experimental design, and data analysis to fulfill MIAPE standards, increase plant proteome coverage and expand biological knowledge
Jesús V. Jorrín-Novo,Ana M. Maldonado,Sira Echevarría-Zomeño,Luis Valledor,Mari Angeles Castillejo,Miguel Curto,Jose Valero,Besma Sghaier,Gabriel Donoso,Inmaculada Redondo +9 more
TL;DR: Most of the papers published deal with the proteome of Arabidopsis thaliana and rice (Oryza sativa), and focus on profiling organs, tissues, cells or subcellular proteomes, and studying developmental processes and responses to biotic and abiotic stresses using a differential expression strategy.
Journal ArticleDOI
Chilling stress-induced proteomic changes in rice roots
Dong-Gi Lee,Nagib Ahsan,Sang Hoon Lee,Jeung Joo Lee,Jeong Dong Bahk,Kyu Young Kang,Byung-Hyun Lee +6 more
TL;DR: Two-dimensional gel electrophoresis coupled with mass spectrometry has been adopted to investigate the protein expression patterns of rice roots in response to chilling stress, and a group of novel proteins were identified.
Journal ArticleDOI
Glyphosate-induced oxidative stress in rice leaves revealed by proteomic approach.
Nagib Ahsan,Dong-Gi Lee,Ki-Won Lee,Iftekhar Alam,Sang Hoon Lee,Jeong Dong Bahk,Byung-Hyun Lee +6 more
TL;DR: An increased accumulation of antioxidant enzymes including ascorbate peroxidase, glutathione S-transferase, thioredoxin h-type, nucleoside diphosphate kinase 1, peroxiredoxin and a superoxide dismutlast precursor in the glyphosate-treated sample suggests that a glyphosate treatment possibly generates oxidative stress in plants.
References
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