A Coupled Assay System for the Lysis of Cyanobacteria.
Hideaki Uchida,Takanori Kouchiwa,Kaori Watanabe,Ayako Kawasaki,Yoshikuni Hodoki,Ikuko I. Ohtani,Yoko Yamamoto,Makoto Suzuki,Ken-ichi Harada +8 more
TLDR
CA has the number of advantages over SAO when considering practical use, however, SAO is indispensable for qualitative analysis, the elucidation of the mode of action and relationship between structure and activity.Abstract:
An assay system was described for studying the mechanism for the lysis of cyanobacteria, which consists of (1) the improved soft-agar overlayer (SAO) method, and (2) the chlorophyll absorbance (CA) method. In the SAO, the activity was observed on a following double-layer agar plate; the agar was covered with a soft agar containing precultured Microcystis aeruginosa in a schale. Lytic plaques on the agar plate were observed using the disk method and/or cup method. In the CA, the cultured cyanobacterium was centrifuged and an aliquot of the concentrated suspension was poured into each well of a 96-well microplate. Subsequently, sample solution was added to the well and the plate was settled. The plates were daily measured for observing any increase or decrease in the OD665. CA has the number of advantages over SAO when considering practical use, however, SAO is indispensable for qualitative analysis, the elucidation of the mode of action and relationship between structure and activity. This method was applied to screen lytic compounds in a lake and two amino acids were found to have lytic activity.read more
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Degradation of microcystins using immobilized microorganism isolated in an eutrophic lake.
TL;DR: An over 80% removal efficiency continued for 2 months, showing that the life of the immobilized B-9 in terms of activity was at least 2 months under the optimized conditions, and is feasible for the practical treatment of microcystins in non-point areas and water purification plants.
Journal ArticleDOI
Lysis of cyanobacteria with volatile organic compounds.
Keiko Ozaki,Akemi Ohta,Chieko Iwata,Aki Horikawa,Kiyomi Tsuji,Emiko Ito,Yoshitomo Ikai,Ken-ichi Harada +7 more
TL;DR: One of bacteria collected from Lake Sagami, Japan, Brevibacillus sp.
Journal ArticleDOI
Distribution and identification of actinomycetes lysing cyanobacteria in a eutrophic lake
Yoko Yamamoto,Takanori Kouchiwa,Yoshikuni Hodoki,Kunimoto Hotta,Hideaki Uchida,Ken-ichi Harada +5 more
TL;DR: Among 83 actinomycete strains isolated from lake sediments, one strain, identified as Streptomyces phaeofaciens, grew well on lawns of living cyanobacteria and rapidly lysed the cyanobacterial cells, found to be one cause of lysis.
Journal ArticleDOI
β-Cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward cyanobacteria
Kazuhiro Yoshikawa,Kyoko Adachi,Miyuki Nishijima,Takahide Takadera,Seiji Tamaki,Ken-ichi Harada,Kenichi Mochida,Hiroshi Sano +7 more
TL;DR: This is the first report on bacteria that produce CNAlA without a supply of the cyanide ion in the medium, suggesting the wide distribution of CNAla production by marine bacteria.
Journal ArticleDOI
Blue Color Formation of Cyanobacteria with β-Cyclocitral
Ken-ichi Harada,Keiko Ozaki,Sayaka Tsuzuki,Hajime Kato,Masateru Hasegawa,Emília Kiyomi Kuroda,Emília Kiyomi Kuroda,Suzue Arii,Kiyomi Tsuji +8 more
TL;DR: Support is provided that β-cyclocitral derived from cyanobacteria plays an important role in the lysis of cyanob bacteria and participates in the blue color formation under natural conditions.
References
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Journal ArticleDOI
Determination ofD-amino acids. II. Use of a bifunctional reagent, 1,5-difluoro-2,4-dinitrobenzene
TL;DR: In this paper, a mixture of 1,5-difluoro-2,4-dinitrophenyl-5-l-alanine amide has been synthesized in high yield from 1,1-fluoro 2,4dinitrobenzene and l-Ala-NH2.
Journal ArticleDOI
The toxins of cyanobacteria.
TL;DR: On May 2, 1878, George Francis of Adelaide, Australia, published the first scholarly description of the potentially lethal e-ects produced by cyanobacteria, sometimes called blue-green algae or, more colloquially, pond scum.
Journal ArticleDOI
A Nonempirical Method Using LC/MS for Determination of the Absolute Configuration of Constituent Amino Acids in a Peptide: Combination of Marfey's Method with Mass Spectrometry and Its Practical Application
TL;DR: In this article, an advanced version of the Marfey's method was proposed to determine the absolute configuration of constituent amino acids in a peptide using liquid chromatography/mass spectrometry (LC/MS).
Journal ArticleDOI
Distribution and identification of actinomycetes lysing cyanobacteria in a eutrophic lake
Yoko Yamamoto,Takanori Kouchiwa,Yoshikuni Hodoki,Kunimoto Hotta,Hideaki Uchida,Ken-ichi Harada +5 more
TL;DR: Among 83 actinomycete strains isolated from lake sediments, one strain, identified as Streptomyces phaeofaciens, grew well on lawns of living cyanobacteria and rapidly lysed the cyanobacterial cells, found to be one cause of lysis.
Journal ArticleDOI
Bacillus volatiles antagonize cyanobacteria
S.J.L. Wright,R.Jane Thompson +1 more
TL;DR: Evidence is presented showing that commonly isolated Bacillus spp.