Journal ArticleDOI
A new method for estimating phytoplankton growth rates and carbon biomass
D. G. Redalje,Edward A. Laws +1 more
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Application of the method to the phytoplankton community in an eutrophic estuary in Hawaii indicates that the cells are growing with a doubling time of about 2 d and that about 85% of the particulate carbon consists of phytopsized carbon.Abstract:
A new method is described for the determination of phytoplankton growth rates and carbon biomass. This procedure is easy to apply and utilizes the labeling of chlorophyll a (chl a) with 14C. Pure chl a is isolated using two-way thin-layer chromatography, and the specific activity of chl a carbon is determined. Data from laboratory cultures indicate that the specific activity of chl a carbon becomes nearly equal to that of total phytoplankton carbon in incubations lasting 6 to 12 h and can be used to calculate phytoplankton growth rates and carbon biomass. Application of the method to the phytoplankton community in an eutrophic estuary in Hawaii indicates that the cells are growing with a doubling time of about 2 d and that about 85% of the particulate carbon consists of phytoplankton carbon.read more
Citations
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A dynamic regulatory model of phytoplanktonic acclimation to light, nutrients, and temperature
TL;DR: A new regulatory model can describe acclimation of phytoplankton growth rate, chlorophyll a : carbon ratio and nitrogen: carbon ratio to irradiance, temperature and nutrient availability and treats respiration as the sum of the maintenance metabolic requirement and the cost of biosynthesis.
Journal ArticleDOI
LIGHT AND TEMPERATURE DEPENDENCE OF THE CARBON TO CHLOROPHYLL a RATIO IN MICROALGAE AND CYANOBACTERIA: IMPLICATIONS FOR PHYSIOLOGY AND GROWTH OF PHYTOPLANKTON
TL;DR: It would appear that use of a single value of θ for phytoplankton is inappropriate for ecological studies and the available data indicate that θ is about three times larger in dinoflagellates than in other algae under comparable PFDs at 20 °C.
Journal ArticleDOI
Biogeochemical significance of bacterial biomass in the ocean's euphotic zone
Byung Cheol Cho,Farooq Azam +1 more
TL;DR: Bacterial abundance and biomass were extensively measured in the euphotic zone at several oligotrophic and mesotrophic sites of coastal and open Pacific ocean during different seasons Cornpansons with phytoplankton biomass and l a t h particulate organic carbon (POC) were made in order to determine the quantitative significance of bacteria as a carbon and nitrogen pool as discussed by the authors.
Journal ArticleDOI
The role of phytoplankton photosynthesis in global biogeochemical cycles.
TL;DR: Calculations of net carbon uptake by the oceans resulting from phytoplankton photosynthesis suggest that without a supply of nutrients external to the ocean, carbon fixation in the open ocean is not presently a significant sink for excess atmospheric CO2.
Journal ArticleDOI
Consistent fractionation of 13C in nature and in the laboratory: growth-rate effects in some haptophyte algae.
Robert R. Bidigare,Arnim Fluegge,Arnim Fluegge,Katherine H. Freeman,Kristi L. Hanson,John M. Hayes,John M. Hayes,David J. Hollander,David J. Hollander,John P. Jasper,Linda L. King,Edward A. Laws,Jeffrey C. Milder,Frank J. Millero,Richard D. Pancost,Brian N. Popp,Paul A. Steinberg,Stuart G. Wakeham +17 more
TL;DR: Before concentrations of dissolved CO2 can be estimated from isotopic fractionations, some means of accounting for variations in growth rate must be found, perhaps by drawing on relationships between [PO4] and Cd/Ca ratios in shells of planktonic foraminifera.
References
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Journal ArticleDOI
New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton
S.W. Jeffrey,G.F. Humphrey +1 more
TL;DR: New equations are presented for spectrophotometric determination of chlorophylls, based on revised extinction coefficients of chloropylls a, b, c1 and c2, which may be used for determining chlorophyLLs a and b in higher plants and green algae.
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Estimating the organic carbon content of phytoplankton from cell volume or plasma volume1
TL;DR: In this article, the organic carbon in phytoplankton from cell volume or plasma volume is derived from original data on five species of diatoms and from data in the literature.