A Novel Agar Medium to Detect Hydrogen Peroxide-Producing Bacteria Based on the Prussian Blue-Forming Reaction
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TLDR
Using a novel agar medium, colonies of H2O2‐producing species including Streptococcus pyogenes were easily identified by the appearance of blue halos and the utility of the medium was illustrated by its successful application to the isolation of H1N1‐producing mutants from a non‐H2O 2‐producing stain of S.pyogenes.Abstract:
The classic method for H(2)O(2) detection involving Prussian blue formation was adapted to formulate a novel agar medium that makes possible in situ detection of H(2)O(2) produced by bacteria. Using this medium, colonies of H(2)O(2)-producing species including Streptococcus pyogenes were easily identified by the appearance of blue halos. The utility of the medium was further illustrated by its successful application to the isolation of H(2)O(2)-producing mutants from a non-H(2)O(2)-producing stain of S. pyogenes.read more
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Increased production of hydrogen peroxide by Lactobacillus delbrueckii subsp. bulgaricus upon aeration: involvement of an NADH oxidase in oxidative stress.
TL;DR: Aeration caused the bacteria to enter early into stationary phase, thus reducing markedly the biomass production but without modifying the maximum growth rate, and indicated that NADH oxidase had no (or a very small) energetic role and could be involved in eliminating oxygen.
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TL;DR: Data show that NOXase is important for aerobic metabolism and essential in environments high in O(2) with carbohydrate limitation.
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Hydrogen Peroxide Production in Streptococcus pyogenes: Involvement of Lactate Oxidase and Coupling with Aerobic Utilization of Lactate
TL;DR: Results suggest that the lactate oxidase activity of H2O2-producing cells oxidizes lactate to pyruvate, which is in turn converted to acetate, and this latter process proceeds presumably via acetyl coenzyme A and acetyl phosphate with formation of extra ATP.