Journal ArticleDOI
A PVC-based electrode sensitive to DDA+ as a device for monitoring the membrane potential in biological systems
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TLDR
It was found that the liposome described above is a good model for the generation of membrane potentials in mitochondria and the electrode lifetime was remarkably prolonged.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1978-04-30. It has received 96 citations till now. The article focuses on the topics: Membrane potential & Membrane.read more
Citations
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Membrane potential of mitochondria measured with an electrode sensitive to tetraphenyl phosphonium and relationship between proton electrochemical potential and phosphorylation potential in steady state.
TL;DR: The kinetics for uptake by mitochondria of TPP+ and DDA+ were analyzed, and it was found that TPP+ permeated the mitochondrial membrane about 15 times faster than DDA+.
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Pediocin PA-1, a Bacteriocin from Pediococcus acidilactici PAC1.0, Forms Hydrophilic Pores in the Cytoplasmic Membrane of Target Cells
Michael L. Chikindas,Maria J. García-Garcerá,Arnold J. M. Driessen,Aat M. Ledeboer,Jon Nissen-Meyer,Ingolf F. Nes,T Abee,Wilhelmus Konings,Gerhardus Venema +8 more
TL;DR: The data suggest that pediocin PA-1 functions in a voltage-independent manner but requires a specific protein in the target membrane, while it is less effective with membranes derived from immune cells.
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Generation of a Proton Motive Force by Histidine Decarboxylation and Electrogenic Histidine/Histamine Antiport in Lactobacillus buchneri
TL;DR: The data suggest that the generation of metabolic energy from histidine decarboxylation results from an electrogenic histidine/histamine exchange and indirect proton extrusion due to the combined action of the decar boxylase and carrier-mediated exchange.
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The purified Ca2+ pump of human erythrocyte membranes catalyzes an electroneutral Ca2+-H+ exchange in reconstituted liposomal systems.
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The bacteriocin lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner.
TL;DR: The combined results obtained with cells, vesicles, and liposomes suggest that the specificity of lactococcin A may be mediated by a receptor protein associated with the cytoplasmic membrane.
References
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Journal ArticleDOI
Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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Chemiosmotic coupling in oxidative and photosynthetic phosphorylation
TL;DR: The end result of the coupling between the flows through the o/r and h/d pathways in oxidative phosphorylation in mitochondria is that, for the equivalent of each pair of electrons traversing the respiratory chain, up to 3 anhydro-bond equivalents may normally traverse the h/D pathway from adenosine diphosphate plus inorganic phosphate (ADP +Pi) to water.
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Safranine as a probe of the mitochondrial membrane potential
TL;DR: The membrane potential has been suggested to be the major component of the electrochemical proton gradient under most conditions, and measurements of this potential are therefore essential for the understanding of the mechanism of oxidative phosphorylation and mitochondrial ion transport.
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The Influence of Respiration and ATP Hydrolysis on the Proton‐Electrochemical Gradient across the Inner Membrane of Rat‐Liver Mitochondria as Determined by Ion Distribution
TL;DR: The extra-mitochondrial phosphate potential sustainable by respiration was found to change in parallel to Δp, but to exceed the latter parameter when based upon a stoichiometry of two protons translocated per ATP synthesised.
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