Journal ArticleDOI
A reference method for measurement of alkaline phosphatase activity in human serum.
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TLDR
An official AACC reference method for the measurement of alkaline phosphatase is presented, the culmination of optimization experiments conducted by a group of independent laboratories.Abstract:
We present an official AACC reference method for the measurement of alkaline phosphatase, the culmination of optimization experiments conducted by a group of independent laboratories. The details of this method and evaluation of factors affecting the measurement are described. A metal ion buffer has been incorporated that maintains optimal and constant concentrations of zinc(II) and magnesium(II) ions. Final reaction conditions are: pH (30 degrees C), 10.40 +/- 0.05; 2-amino-2-methyl-1-propanol buffer, 0.35 mol/L; 4-nitrophenyl phosphate, 16.0 mmol/L; magnesium acetate, 2.0 mmol/L; zinc sulfate, 1.0 mmol/L; and N-(2-hydroxyethyl)ethylenediaminetriacetic acid, 2.0 mmol/L.read more
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Journal ArticleDOI
Role of nutrients in skeletal metabolism and pathology in fish — An overview
TL;DR: Bone deformities associated with nutrient deficiencies and toxicities in various fish can provide an excellent model to study gene action, cell differentiation, morphogenesis, species differences in phenotypic expression of genetic abnormalities, deposition of calcium, phosphorus and other trace elements in cartilage and skeletal tissues in response to vitamins and hormones and nutrient interactions.
Journal ArticleDOI
IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. International Federation of Clinical Chemistry and Laboratory Medicine. Part 4. Reference procedure for the measurement of catalytic concentration of alanine aminotransferase.
Gerhard Schumann,R. Bonora,Ferruccio Ceriotti,Georges Férard,Carlo A. Ferrero,Paul F H Franck,F. Javier Gella,Wieland Hoelzel,Poul J. Jørgensen,Takashi Kanno,Art Kessner,Rainer Klauke,N Kristiansen,Jean-Marc Lessinger,Thomas P. J. Linsinger,Hideo Misaki,Mauro Panteghini,Jean Pauwels,Françoise Schiele,Heinz Schimmel,Gerhard Weidemann,Lothar Siekmann +21 more
TL;DR: The procedure described here is deduced from the previously described 30°C IFCC reference method, and differences are tabulated and commented on in Appendix 2.
Journal ArticleDOI
Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats.
TL;DR: It could be concluded that spirulina administration able to minimize the toxic effects of deltamethrin by its free radical-scavenging and potent antioxidant activity.
Journal ArticleDOI
Regulated production of mineralization-competent matrix vesicles in hypertrophic chondrocytes.
TL;DR: The findings reveal that hypertrophic chondrocytes can qualitatively modulate their production of matrix vesicles and only when induced to initiate mineralization, will release mineralization-competent matrixVesicles rich in annexin V and alkaline phosphatase.
Journal ArticleDOI
A randomized, double-blind comparison of risedronate and etidronate in the treatment of paget’s disease of bone
Paul D. Miller,Jacques P. Brown,Ethel S. Siris,Mohammad S. Hoseyni,Douglas Wayne Axelrod,Pirow J. Bekker +5 more
TL;DR: Although etidronate is effective, risedronate offers a shorter duration of therapy, better and longer-lasting remission, significant reductions in pain, and provides additional remission in subjects who exhibited an incomplete response to previous etidronsate treatment.
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