Journal ArticleDOI
A Requirement for Two Cell Types for Antibody Formation in vitro
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It was found that both adherent and nonadherent cells were necessary for the induction of antibody formation to sheep red blood cells in vitro.Abstract:
A suspension of mouse spleen cells can be separated into two populations on the basis of their ability or inability to adhere to plastic dishes. It was found that both adherent and nonadherent cells were necessary for the induction of antibody formation to sheep red blood cells in vitro. Exposure of adherent cells to antigen for brief periods of time was sufficient to initiate a maximal in vitro response.read more
Citations
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The dendritic cell system and its role in immunogenicity
TL;DR: Dendritic cells are specialized to mediate several physiologic components of immunogenicity such as the acquisition of antigens in tissues, the migration to lymphoid organs, and the identification and activation of antigen-specific T cells.
Journal ArticleDOI
Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution.
Ralph M. Steinman,Zanvil A. Cohn +1 more
TL;DR: Dendritic cells represent a novel cell type on both functional and morphological grounds and do not possess the functional properties of other types of reticular cells proposed to exist in lymphoid organs.
Journal ArticleDOI
The basis for the immunoregulatory role of macrophages and other accessory cells.
Emil R. Unanue,Paul M. Allen +1 more
TL;DR: Macrophages handle extracellular proteins and secrete diverse bioactive molecules and, therefore, influence the physiology of many tissues and have an important immunoregulatory role.
Journal ArticleDOI
Identification of a novel cell type in peripheral lymphoid organs of mice
Ralph M. Steinman,Zanvil A. Cohn +1 more
TL;DR: Several properties of lymphoid dendritic cells in situ have been determined, and contrasted to information previously established for lymphocytes and mononuclear phagocytes as mentioned in this paper, such as the mature splenic population does not actively divide (pulse labeling index with [3H]thymidine of 1.5-2.5%), but does turnover at substantial rate, 10+% of the total pool per day.
Journal ArticleDOI
Potentiation of the t-lymphocyte response to mitogens ii. the cellular source of potentiating mediator(s)
Igal Gery,Byron H. Waksman +1 more
TL;DR: It is concluded that LAF stimulates both central and peripheral T lymphocytes and enhances their responses to other stimulants.
References
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Journal ArticleDOI
Plaque Formation in Agar by Single Antibody-Producing Cells
Niels K. Jerne,Albert A. Nordin +1 more
TL;DR: Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of Sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.
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Immunization of dissociated spleen cell cultures from normal mice
TL;DR: There is an increase in the ability to discriminate between different varieties of homologous erythrocytes and the in vitro response does not appear to be limited by whatever mechanisms regulate the in vivo response.
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Antibody formation initiated in vitro. II. Antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen.
M. Fishman,F. L. Adler +1 more
TL;DR: The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage.
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Immunogenicity of antigen-containing ribonucleic acid preparations from macrophages.
TL;DR: The arrival directions of the solar particles aro such that they may first be trapped by the magnetic field of the Earth, then by the fields of the test magnets, and finally by this energy selection process exert a greater influence on the reproductive responses of flies in the Magnetic field than in control cultures.
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Immunization of Normal Mouse Spleen Cell Suspensions in vitro
TL;DR: Spleen cell suspensions cultured with heterologous red cells developed levels of hemolytic plaque-forming cells only one log2 less than those seen in vivo, indicating the reaction is specific for the in vitro immunizing erythrocytes.