Journal ArticleDOI
A reversed-phase compatible thin-layer chromatography autography for the detection of acetylcholinesterase inhibitors.
TLDR
The developed assay is able to detect acetylcholinesterase inhibitors present in complex matrixes that were chromatographed in normal phase or reversed-phase thin-layer chromatography.Abstract:
A dual readout autographic assay to detect acetylcholinesterase inhibitors present in complex matrices adsorbed on reversed-phase or normal-phase thin-layer chromatography plates is described. Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The effects of substrate and enzyme concentrations, pH, incubation time, and incubation temperature on the sensitivity and the detection limit of the assay were evaluated. Experimental design and response surface methodology were used to optimize conditions with a minimum number of experiments. The assay allowed the detection of 0.01% w/w of physostigmine in both a spiked Sonchus oleraceus L. extract chromatographed on normal phase and a spiked Pimenta racemosa (Mill.) J.W. Moore leaf essential oil chromatographed on reversed phase. Finally, the reversed-phase thin-layer chromatography assay was applied to reveal the presence of an inhibitor in the Cymbopogon citratus (DC.) Stapf essential oil. The developed assay is able to detect acetylcholinesterase inhibitors present in complex matrixes that were chromatographed in normal phase or reversed-phase thin-layer chromatography. The detection limit for physostigmine on both normal and reversed phase was of 1×10(-4) μg. The results can be read by a change in color and/or a change in fluorescence.read more
Citations
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Automated piezoelectric spraying of biological and enzymatic assays for effect-directed analysis of planar chromatograms.
TL;DR: In this article, the authors compared the performance of piezoelectric spraying with those of immersion and found that the applied solutions were almost comparable, and that the simplicity remains the key argument for immersion.
Journal ArticleDOI
Recent Advances in Effect-directed Enzyme Assays based on Thin-layer Chromatography.
Sarah Bräm,Evelyn Wolfram +1 more
TL;DR: This review summarises recently introduced TLC bioautographic enzyme assays as well as advances in already existing procedures for screening of secondary metabolite profiles for potential enzyme and enzyme inhibitory activities.
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Selective two-dimensional effect-directed analysis with thin-layer chromatography.
TL;DR: This study focused on the development of selective two-dimensional (2D) HPTLC-EDA to increase the peak capacity and facilitate the identification of effective compounds.
Journal ArticleDOI
Reverse Phase Compatible TLC‐Bioautography for Detection of Tyrosinase Inhibitors
TL;DR: A bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase TLC-plates that can be used for bioguided isolation of bioactive compounds is developed.
Journal ArticleDOI
New planar assay for streamlined detection and quantification of β-glucuronidase inhibitors applied to botanical extracts
TL;DR: The hyphenation of the high performance thin layer chromatography technique with a β-glucuronidase inhibition assay was investigated and successfully demonstrated for the first time, covering four orthogonal dimensions, i.e. separation, spectral detection, biochemical activity and structural characterization, in a highly targeted time- and material-saving workflow for analysis of complex or costly mixtures.
References
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In situ-forming hydrogels--review of temperature-sensitive systems.
TL;DR: This manuscript focuses on aqueous polymeric solutions that form implants in situ in response to temperature change, generally from ambient to body temperature, and mainly reviews the characterization and use of polysaccharides, N-isopropylacrylamide copolymers, poly(ethylene oxide) (poloxamer) and itsCopolymers.
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Acetylcholinesterase inhibitors from plants
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Screening for acetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining.
TL;DR: Thin-layer chromatography was used to screen for acetylcholinesterase inhibitors from Amaryllidaceae extracts and could dereplicate the known inhibitor galanthamine at an early stage of the isolation procedure, making this method more sensitive than UV or Dragendorff's reagent detection.
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A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants
TL;DR: A simple and rapid bioautographic enzyme assay on TLC plates has been developed for the screening of acetylcholinesterase and butyrylcholiersterase inhibition by plant extracts.
Journal ArticleDOI
A Study of the Temperature-Dependent Micellization of Pluronic F127.
TL;DR: The temperature-dependent micellization of the pharmaceutically important PEO-PPO-PEO copolymer, Pluronic F127 was examined using static light scattering and various aspects of the pyrene fluorescence spectrum (monomer intensity, excimer formation and the I1/I3 ratio), and results agreed with those reported in the literature.