Journal ArticleDOI
A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites
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A protocol is described for rapid RNA isolation from various plant species and tissues rich in polyphenolics and polysaccharides based on the Nucleon PhytoPure™ system without the use of phenol.Abstract:
A protocol is described for rapid RNA isolation from various plant species and tissues rich in polyphenolics and polysaccharides. The method is based on the Nucleon PhytoPure™ system without the use of phenol. The procedure can be completed within 1 h and many samples can be processed at the same time. The yield ranged from 240 μg up to 3 mg per gram of tissue with an average purity measured as A260/280 of 1.85. The RNA was of sufficient quality for use in RT-PCR reactions. Quantitation of single-stranded cDNA was carried out with the RiboGreen™ reagent and of PCR products with the PicoGreen™ reagent.read more
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Abramoff MD, Magalhaes PJ, Ram SJ. 2004. Image Processing with ImageJ. Biophotonics
Journal ArticleDOI
Interplant communication of tomato plants through underground common mycorrhizal networks.
TL;DR: This finding indicates that CMNs may function as a plant-plant underground communication conduit whereby disease resistance and induced defence signals can be transferred between the healthy and pathogen-infected neighbouring plants, suggesting that plants can ‘eavesdrop’ on defence signals from the Pathogen-challenged neighbours through CMNs to activate defences before being attacked themselves.
Journal ArticleDOI
An RNA isolation system for plant tissues rich in secondary metabolites.
Sanjay Ghawana,Asosii Paul,Hitesh Kumar,Hitesh Kumar,Arun Kumar,Harsharan Singh,Harsharan Singh,Pardeep Kumar Bhardwaj,Arti Rani,Ravi S. Singh,Jyoti Raizada,Kashmir Singh,Kashmir Singh,Sanjay Kumar +13 more
TL;DR: A rapid protocol for isolation of RNA, which works well with all the tissues examined so far, and was amenable to downstream applications such as reverse transcription-polymerase chain reaction (RT-PCR), differential display (DD), suppression subtractive hybridization (SSH) library construction, and northern hybridization.
Journal ArticleDOI
Enhanced tomato disease resistance primed by arbuscular mycorrhizal fungus.
TL;DR: This study found that mycorrhizal inoculation with AMF Funneliformis mosseae significantly alleviated tomato (Solanum lycopersicum Mill.) early blight disease caused by Alternaria solani Sorauer and suggested that the JA signaling pathway is essential for myCorrhiza-primed disease resistance.
Journal ArticleDOI
Isolation of high-quality RNA from gymnosperm and angiosperm trees.
Natalia Kolosova,Barbara Miller,Steven G. Ralph,Brian E. Ellis,Carl J. Douglas,Kermit Ritland,Jörg Bohlmann +6 more
TL;DR: An improved protocol was developed for efficient and reliable extraction of high-quality total RNA and mRNA from various tissues of spruce and poplar trees, as well as other plant species, and suitable for standard expressed sequence tags (ESTs), full-length cDNA library construction, and for microarray applications.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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A simple and efficient method for isolating RNA from pine trees
Book
Plant Molecular Biology Manual
TL;DR: This second edition of the Plant Molecular Biology Manual contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, and nuclear protein targeting, not previously described in the first edition.
Journal ArticleDOI
A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.).
C.Y. Wan,T.A. Wilkins +1 more
TL;DR: The unsurpassed qualitative and quantitative recovery of total RNA from cotton by hot borate buffer at alkaline pH, supplemented with PVP-40, deoxycholate, and/or NP-40 had also proven satisfactory for other recalcitrant plant species as well as for especially difficult tissue types.
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PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR.
TL;DR: PicoGreen (Molecular Probes, Eugene, OR) fluorescence enhancement as a useful assay for template DNA quantitation and PCR product formation is discussed and PicoGreen is evaluated for quantitation of multiple DNA sample types.