Journal ArticleDOI
A small cosmid for efficient cloning of large DNA fragments.
Barbara Hohn,John Collins +1 more
TLDR
The production and use of the 6 kb cosmid pHC79, a derivative of pBR322, is described, which can be used for cloning of fragments cleaved by EcoRI, ClaI, BamHI, SalI (also XhoI and AvaI), EcaI and PstI.About:
This article is published in Gene.The article was published on 1980-11-01. It has received 773 citations till now. The article focuses on the topics: Cosmid Vector & Cosmid.read more
Citations
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The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector
TL;DR: It was found that the gene 5 promoter is active in a tissue-specific fashion whereas this is not the case for the NOS promoter, providing the first documented instance of a gene derived from a procaryotic host the expression of which is apparently regulated by plant growth factors.
Journal ArticleDOI
Common virulence factors for bacterial pathogenicity in plants and animals
Laurence G. Rahme,Emily J. Stevens,Sean F. Wolfort,Jing Shao,Ronald G. Tompkins,Frederick M. Ausubel +5 more
TL;DR: A Pseudomonas aeruginosa strain (UCBPP-PA14) is infectious both in an Arabidopsis thaliana leaf infiltration model and in a mouse full-thickness skin burn model, indicating that these genes encode virulence factors required for the full expression of pathogenicity in both plants and animals.
Journal ArticleDOI
Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
Miroslawa M. Bagdasarian,Rudolf Lurz,Beate Rückert,F. C. H. Franklin,Michael Bagdasarian,Jürgen Frey,K.N. Timmis +6 more
TL;DR: A cosmid that may be used for the selective cloning of large DNA fragments by in vitro lambda packaging and an analogous series of vectors that are defective in their plasmid-mobilization function, and that exhibit a degree of biological containment comparable to that of current Escherichia coli vector plasmids, are described.
Journal ArticleDOI
Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria
Jürgen Quandt,Michael F. Hynes +1 more
TL;DR: A set of vector plasmids which greatly facilitate gene replacement and reverse genetics in many Gram-negative bacteria was constructed and it is demonstrated that they are extremely useful in eliminating long and tedious screening procedures.
Journal ArticleDOI
The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.
TL;DR: It is suggested that the hypervirulence of Agrobacterium tumefaciens A281 is due to non-T-DNA sequences on the Ti plasmid.
References
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Book
Genetic Engineering: Principles and Methods
TL;DR: Regulation of Plant Intercellular Communication Via Plasmodesmata and Paired-End Genomic Signature Tags are described as a Method for the Functional Analysis of Genomes and Epigenomes.
Journal ArticleDOI
Plasmid screening at high colony density
Douglas Hanahan,Matthew Meselson +1 more
TL;DR: A procedure is described for screening bacterial colonies containing recombinant plasmids by nucleic acid hybridization at high density, i.e., at 100 000 colonies per 150 mm diameter plate.
Journal ArticleDOI
Charon phages: safer derivatives of bacteriophage lambda for DNA cloning
Frederick R. Blattner,Bill G. Williams,Ann E. Blechl,Katherine Denniston-Thompson,Harvey E. Faber,Lesley Anne Furlong,David Grunwald,Delight O. Kiefer,David D. Moore,James W. Schumm,Edward L. Sheldon,Oliver Smithies +11 more
TL;DR: Three of the Charon vector phages, 3A, 4A, and 16A, have been certified for use as EK2 vector-host systems, when propagated in bulk in a special bacterial host, DP50SupF.
Journal ArticleDOI
Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.
Jack R. Collins,Barbara Hohn +1 more
TL;DR: Evidence is presented that ColE1 hybrid plasmids carrying the cohesive-end site (cos) of lambda can be used as gene cloning vectors in conjunction with the lambda in vitro packaging system.
Journal ArticleDOI
Packaging recombinant DNA molecules into bacteriophage particles in vitro.
Barbara Hohn,Kenneth Murray +1 more
TL;DR: Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro and is efficient and nonselective and offers containment in initial stages of handling recombinant DNA.