Aconitase and citric acid fermentation by Aspergillus niger.
C P Kubicek,M Röhr +1 more
TLDR
Compared in vivo steady-state concentrations of citrate and isocitrate in Aspergillus niger grown under various citric acid-producing conditions strongly argue against an inhibition of aconitase duringcitric acid fermentation.Abstract:
In view of the often-cited theory that citric acid accumulation is caused by an inhibition of aconitase activity, the equilibrium of the reaction of aconitase was investigated by comparing in vivo steady-state concentrations of citrate and isocitrate in Aspergillus niger grown under various citric acid-producing conditions. With the equilibrium catalyzed by the A. niger enzyme in vitro, similar values were obtained. The validity of our in vivo measurements was verified by the addition of the aconitase inhibitor fluorocitrate, which appreciably elevated the citrate:isocitrate ratio. The results strongly argue against an inhibition of aconitase during citric acid fermentation.read more
Citations
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Organic Acid Production by Filamentous Fungi
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Fungal production of citric acid.
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References
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Journal ArticleDOI
Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.
TL;DR: A spectrophotometric method of measuring the enzymatic formation and disappearance of umaric and cis -aconitic acids is reported.
Journal ArticleDOI
Influence of manganese on enzyme synthesis and citric acid accumulation inAspergillus niger
C. P. Kubicek,M. Röhr +1 more
TL;DR: The hypothesis that manganese deficiency mainly affects the operation of biosynthetic reactions in Aspergillus niger, thus leading to an overflow of citric acid as an end product of glycolysis is supported.
Journal ArticleDOI
Nitro analogues of citrate and isocitrate as transition-state analogues for aconitase.
Journal ArticleDOI
The role of the tricarboxylic acid cycle in citric acid accumulation by Aspergillus niger
C. P. Kubicek,M. Röhr +1 more
TL;DR: During the growth period the levels of almost all TCA cycle acids, with the exception of 2-oxo-acids, were unusually high; during the induction phase of citrate accumulation malate, fumarate, and isocitrate decreased, whereas pyruvate, oxalacetate, and citrate increased.
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Influence of manganese on enzyme synthesis and citric acid accumulation inAspergillus niger
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