Journal ArticleDOI
Activation tagging of the floral inducer FT.
Igor Kardailsky,Vipula K. Shukla,Ji Hoon Ahn,Nicole Dagenais,Sioux K. Christensen,Jasmine Thuy Nguyen,Joanne Chory,Maria J. Harrison,Detlef Weigel +8 more
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TLDR
FLOWERING LOCUS T (FT), which acts in parallel with the meristem-identity gene LEAFY (LFY) to induce flowering of Arabidopsis, was isolated by activation tagging and the deduced sequence was similar to the sequence of TERMINAL FLOWER 1 (TFL1), an inhibitor of flowering that also shares sequence similarity with membrane-associated mammalian proteins.Abstract:
FLOWERING LOCUS T (FT), which acts in parallel with the meristem-identity gene LEAFY (LFY) to induce flowering of Arabidopsis, was isolated by activation tagging. Like LFY, FT acts partially downstream of CONSTANS (CO), which promotes flowering in response to long days. Unlike many other floral regulators, the deduced sequence of the FT protein does not suggest that it directly controls transcription or transcript processing. Instead, it is similar to the sequence of TERMINAL FLOWER 1 (TFL1), an inhibitor of flowering that also shares sequence similarity with membrane-associated mammalian proteins.read more
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FT Protein Movement Contributes to Long-Distance Signaling in Floral Induction of Arabidopsis
Laurent Corbesier,Coral Vincent,Seonghoe Jang,Fabio Fornara,Qingzhi Fan,Qingzhi Fan,Iain R. Searle,Antonis Giakountis,Sara Farrona,Lionel Gissot,Colin G. N. Turnbull,Colin G. N. Turnbull,George Coupland +12 more
TL;DR: It is concluded that FT protein acts as a long-distance signal that induces Arabidopsis flowering, and evidence that FT does not activate an intermediate messenger in leaves is provided.
Journal ArticleDOI
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
TL;DR: A novel approach for enhancing the accumulation of natural products based on activation tagging by Agrobacterium-mediated transformation with a T-DNA that carries cauliflower mosaic virus 35S enhancer sequences at its right border is reported.
Journal ArticleDOI
FD, a bZIP Protein Mediating Signals from the Floral Pathway Integrator FT at the Shoot Apex
Mitsutomo Abe,Yasushi Kobayashi,Sumiko Yamamoto,Yasufumi Daimon,Ayako Yamaguchi,Yoko Ikeda,Harutaka Ichinoki,Michitaka Notaguchi,Koji Goto,Takashi Araki,Takashi Araki +10 more
TL;DR: FLOWERING LOCUS T (FT) is a conserved promoter of flowering that acts downstream of various regulatory pathways, including one that mediates photoperiodic induction through CONSTANS (CO), and is expressed in the vasculature of cotyledons and leaves.
Journal ArticleDOI
Highly specific gene silencing by artificial microRNAs in Arabidopsis.
TL;DR: The specificity of ami RNAs, as deduced from genome-wide expression profiling, was as high as that of natural plant miRNAs, supporting the notion that extensive base pairing with targets is required for plant miRNA function.
Journal ArticleDOI
Distinct Roles of CONSTANS Target Genes in Reproductive Development of Arabidopsis
Alon Samach,Hitoshi Onouchi,Scott E. Gold,Gary S. Ditta,Zsuzsanna Schwarz-Sommer,Martin F. Yanofsky,George Coupland +6 more
TL;DR: Four early target genes of CO were identified using a steroid-inducible version of the protein to define common components of distinct flowering-time pathways.
References
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Journal ArticleDOI
The CONSTANS gene of arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors
TL;DR: Double mutants were constructed containing co and mutations affecting gibberellic acid responses, meristem identity, or phytochrome function, and their phenotypes suggested a model for the role of CO in promoting flowering.
Journal ArticleDOI
A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana.
TL;DR: A differential response of the mutants to environmental factors and their subdivision into epistatic groups is discussed in relation to a causal model for floral initiation in Arabidopsis thaliana.
Journal ArticleDOI
Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP
Kam C. Yeung,T Seitz,Shengfeng Li,Petra Janosch,Brian McFerran,Christian M. Kaiser,Frances Fee,Kostas D. Katsanakis,David W. Rose,Harald Mischak,John M. Sedivy,Walter Kolch +11 more
TL;DR: RKIP represents a new class of protein-kinase-inhibitor protein that regulates the activity of the Raf/MEK/ERK module and competitively disrupts the interaction between these kinases.
Journal ArticleDOI
Inflorescence Commitment and Architecture in Arabidopsis
TL;DR: The Antirrhinum gene CENTRORADIALIS and the Arabidopsis gene TERMINAL FLOWER 1 (TFL1) were shown to be homologous, which suggests that a common mechanism underlies indeterminacy in these plants.
Journal ArticleDOI
A developmental switch sufficient for flower initiation in diverse plants
TL;DR: Transgenic plants in which the flower-meristem-identity gene LEAFY of Arabidopsis is constitutively expressed are generated, suggesting a new level of regulation during flower development, as indicated by the competence of the main shoot to respond to LEAFy activity.