Activator Specificity of Pyruvate Kinase from Lactic Streptococci
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The pyruvate kinase from lactic streptococci was activated by 20 structurally dissimilar sugar phosphates and glycolytic intermediates and nine compounds were more effective activators than fructose-1,6-diphosphate.Abstract:
The pyruvate kinase from lactic streptococci was activated by 20 structurally dissimilar sugar phosphates and glycolytic intermediates. Nine compounds were more effective activators than fructose-1,6-diphosphate.read more
Citations
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Journal ArticleDOI
Control of the shift from homolactic acid to mixed-acid fermentation in Lactococcus lactis: predominant role of the NADH/NAD+ ratio.
TL;DR: During batch growth of Lactococcus lactis subsp.
Journal ArticleDOI
Bioenergetics and Solute Transport in Lactococci
TL;DR: Detailed studies of the transport processes of lactic acid bacteria during growth and starvation have been performed in cytoplasmic membrane vesicles of these organisms in which a foreign proton pump has been introduced to generate a high proton motive force.
Journal ArticleDOI
Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR
TL;DR: In vivo nuclear magnetic resonance has proven a very useful technique to monitor non-invasively the dynamics of intracellular metabolite and co-factor pools following a glucose pulse and the use of this information to direct metabolic engineering strategies is illustrated.
Journal ArticleDOI
Physiology of pyruvate metabolism in Lactococcus lactis.
TL;DR: It will be demonstrated that the metabolic transformation of pyruvate can be predicted if the growth-limiting constraints are adequately established and the normal pathways can no longer maintain balanced carbon flux.
Journal ArticleDOI
Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools determined in vivo by 13C NMR.
Ana Rute Neves,Rita Ventura,Nahla Mansour,Claire Shearman,Michael J. Gasson,Christopher D. Maycock,Ana Ramos,Helena Santos +7 more
TL;DR: The data are interpreted as showing that the glycolytic flux in wild type L. lactis is not primarily controlled at the level of glyceraldehyde-3-phosphate dehydrogenase by NADH, and the ATP/ADP/P(i) content could play an important role.
References
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Journal ArticleDOI
Mechanisms of Lactose Utilization by Lactic Acid Streptococci: Enzymatic and Genetic Analyses
TL;DR: Lactose-negative mutants of S. lactis 7962, a strain containing beta-galactosidase, could be separated into several classes, which indicated that this organism is not dependent upon the PEP-phosphotransferase system for lactose metabolism.
Journal ArticleDOI
The control of pyruvate kinases of Escherichia coli. I. Physicochemical and regulatory properties of the enzyme activated by fructose 1,6-diphosphate.
E. Bruce Waygood,B. D. Sanwal +1 more
TL;DR: The pyruvate kinase of Escherichia coli activated by fructose 1,6-diphosphate has been purified to homogeneity by electrophoresis on sodium dodecyl sulfate polyacrylamide gels, and ultracentrifugational experiments as discussed by the authors.
Journal ArticleDOI
Lactose and D-galactose metabolism in group N streptococci: presence of enzymes for both the D-galactose 1-phosphate and D-tagatose 6-phosphate pathways.
TL;DR: Group N streptococci possess enzymes for the metabolism of d-Galactose through both the d-galactose 1-phosphate pathway (Leloir pathway) and thed-tagatose 6-ph phosphate pathway.
Book ChapterDOI
The Regulation of Pyruvate Kinase
Werner Seubert,Wilhelm Schoner +1 more
TL;DR: This chapter discusses the molecular and kinetic properties, and the physiological significance of pyruvate kinase, which adds strong support to a physiological importance of both the intracellular pH and acetyl-CoA in the control of glycolysis and gluconeogenesis in liver.
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