Activity of Androstendione on the Sexual Organs of the Male Rat

TLDR: Gallagher and Koch showed that boiling alkali destroys the hormone action of testicular extracts only, but not of the urine extracts, androsterone and trans-dehydro-androsterone, the existence of which in the urine has been described by Butenandt, are not altered by treatment with alkali.

Abstract: It has repeatedly been stated that the relation between the results of capon test and rat test varies with preparations having male hormone action1,2. A definite number of capon units, applied in the form of testicular extracts, has a stronger activity on the sexual organs of the male rat than pure androsterone or hormone fractions fram male urine. Gallagher and Koch2, moreover, showed that boiling alkali destroys the hormone action of testicular extracts only, but not of the urine extracts. In fact, androsterone and trans-dehydro-androsterone, the existence of which in the urine has been described by Butenandt, are not altered by treatment with alkali.