Adenine derivatives as neurohumoral agents in the brain. The quantities liberated on excitation of superfused cerebral tissues.
Ian Pull,Henry McIlwain +1 more
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The hypoxanthine sample derived from superfusate hyp oxanthine, inosine and adenosine was of similar specific radioactivity to the sample of inOSine separated chromatographically, and each was of higher specificRadioactivity than the adenine nucleotides obtained by cold-acid extraction of the tissue.Abstract:
Adenine nucleotides of guinea-pig neocortical tissues were labelled by incubation with [(14)C]adenine and excess of adenine was then removed by superfusion with precursor-free medium. Adenine derivatives released from the tissue during continued superfusion, including a period of electrical stimulation of the tissue, were collected by adsorption and examined after elution and concentration. The stimulation greatly increased the (14)C output, and material collected during and just after stimulation had a u.v. spectrum which indicated adenosine to be a major component. The additional presence of inosine and hypoxanthine was shown by chromatography and adenosine was identified also by using adenosine deaminase. Total adenine derivatives released from the tissue during a 10min period of stimulation were obtained as hypoxanthine, after deamination and hydrolysis of adenosine and inosine, and amounted to 159nmol/g of tissue. This corresponded to the release of approx. 7pmol/g of tissue per applied stimulus. The hypoxanthine sample derived from superfusate hypoxanthine, inosine and adenosine was of similar specific radioactivity to the sample of inosine separated chromatographically, and each was of higher specific radioactivity than the adenine nucleotides obtained by cold-acid extraction of the tissue.read more
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References
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Journal Article
The Effect of Adenosine and Adenine Nucleotides on the Cyclic Adenosine 3',5'-Phosphate Content of Guinea Pig Cerebral Cortex Slices
Albert Sattin,T. W. Rall +1 more
TL;DR: Changes in the tissue compartmentation of adenine nucleotides probably play a major role in producing the previously observed increase of cyclic adenosine 39,59-phosphate during electrical stimulation of slices.
Journal ArticleDOI
Evidence that adenosine triphosphate or a related nucleotide is the transmitter substance released by non-adrenergic inhibitory nerves in the gut
TL;DR: It is suggested that ATP or a related nucleotide is the transmitter substance released by the non‐adrenergic inhibitory innervation of the gut.
Journal ArticleDOI
A radioisotopic method for measuring the formation of adenosine 3',5'-cyclic monophosphate in incubated slices of brain.
TL;DR: The method was used to study the effect of histamine, norepinephrine, andAdenosine on the accumulation of adenosine 3′,5′‐cyclic monophosphate in incubated slices of brain.
Journal ArticleDOI
The effect of electrical stimulation upon the accumulation of adenosine 3',5'-phosphate in isolated cerebral tissue.
TL;DR: The application of electrical pulses to slices of guinea pig cerebral cortex led to an increase in the levels of adenosine 3′,5′‐phosphate (cyclic 3‐fold) of more than 11‐fold within 10 min, which cannot be ascribed to the release and action of either histamine or norepinephrine.
Journal ArticleDOI
Biochemistry and the Central Nervous System
TL;DR: Biochemistry and the central nervous system always becomes the most wanted book.
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