Journal ArticleDOI
An Antisense Plasmid Targeting Survivin Expression Induces Apoptosis and Sensitizes Hepatocarcinoma Cells to Chemotherapy
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TLDR
The threshold of apoptosis was decreased after survivin was silenced, and the sensitivity to chemotherapy was increased, suggesting the existence of a potential new target for gene therapy.Abstract:
To explore the change of sensitivity to chemotherapy of antisense RNA targeting survivin on hepatocarcinoma carcinoma cellsin vitro. Survivin mRNA structure region was amplified by RT-PCR and inserted inversely into eukaryotic expression vector pcDNA3. The antisense expression plasmid pcDNA3/survivin was transfected into HepG2 with lipofectAMINETM, 2000 (FL2000), with low concentration of 5-fluorouracil (5-Fu) added. Survivin protein was detected by westernblot, the growth activity was measured by MTT, and apoptosis was detected by Flow cytometry 12 h, 24 h, 48 h after transfection. The activity of caspase-3 was found by quantitative assay 48 h after transfection. The construction of antisense RNA vector pcDNA3/survivin was verified by restricted endonuclease digestion and nucleotide sequencing. Compared with normal group, 5-Fu and antisense survivin group, the cells growth inhibition, apoptosis index, and caspase-3 activity were increased in antisense survivin transfected + 5-Fu group. The threshold of apoptosis was decreased after survivin was silenced, and the sensitivity to chemotherapy was increased. These findings suggest the existence of a potential new target for gene therapy.read more
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Survivin expression in breast cancer predicts clinical outcome and is associated with HER2, VEGF, urokinase plasminogen activator and PAI-1
Bríd M. Ryan,Gottfried E. Konecny,S. Kahlert,He-Jing Wang,Michael Untch,G. Meng,Mark D. Pegram,Karl C. Podratz,John Crown,DJ Slamon,Michael J. Duffy +10 more
TL;DR: The independent prognostic relevance of survivin, when combined with previous data from model systems implicating survivin in the inhibition of apoptosis, suggests that survivin may be a suitable target for future therapeutic strategies.
Journal ArticleDOI
Survivin expression and targeting in breast cancer.
TL;DR: Survivin is over expressed in majority of breast cancers and the over expression of survivin is found to correlate with HER 2 and EGFR expression, and has been found to confer resistance to chemotherapy and radiation.
Journal ArticleDOI
Characterization of cell death events induced by anti-neoplastic drugs cisplatin, paclitaxel and 5-fluorouracil on human hepatoma cell lines: Possible mechanisms of cell resistance.
TL;DR: Three drugs tested in this study could induce cell death, with paclitaxel being more effective inducing apoptosis, and 5FU was only effective at high doses and its mechanism seems to be primarily related to necrosis in Hep3B and probably apoptosis in HepG2.
Journal ArticleDOI
Sensitization of osteosarcoma cell line SaOS-2 to chemotherapy by downregulating survivin.
TL;DR: It is found that the downregulation of survivin by pSUPER-sh could enhance the anticancer effects of chemotherapies such as etoposide, cisplatin and doxorubicin through decreasing mitochondrial membrane potentials and increasing caspase-3 activity.
Journal ArticleDOI
Survivin-mediated Therapeutic Efficacy of Gemcitabine through Glucose-regulated Protein 78 in Hepatocellular Carcinoma
Chin Sheng Hung,Shen Fu Lin,Hui Hsiung Liu,Li Jen Kuo,Li Jen Kuo,Li Tzu Li,Hou Yu Su,Hou Yu Su,Phui Ly Liew,Feng Yen Lin,Feng Yen Lin,Po Li Wei,Po Li Wei,Der Zen Liu,Yu Jia Chang,Yu Jia Chang +15 more
TL;DR: It is found that survivin knockdown resulted in a reduction of glucose-regulated protein 78 (GRP78), which may be responsible for the observed increased survivin-KD cell sensitivity to gemcitabine.
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The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins
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