An easy pipeline for one-step purification of SARS-CoV-2 nucleocapsid protein from insect cell suspension culture
Brenda R. de Camargo,Leonardo Assis da Silva,Athos Silva de Oliveira,Bergmann Morais Ribeiro +3 more
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TLDR
In this paper, an easy pipeline for expression of SARS-CoV-2 nucleocapsid (N) protein in insect cells followed by its purification via affinity chromatography was described.About:
This article is published in Journal of Virological Methods.The article was published on 2022-01-01 and is currently open access. It has received 4 citations till now. The article focuses on the topics: Biology & Sf9.read more
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Understanding the Role of SARS-CoV-2 ORF3a in Viral Pathogenesis and COVID-19
Jiantao Zhang,Amara Ejikemeuwa,Volodymyr Gerzanich,M. Nasr,Qiyi Tang,J. Marc Simard,Richard Y. Zhao +6 more
TL;DR: This review provides an in-depth analysis of ORF3a protein’s structure, origin, evolution, and mutant variants, and how these characteristics affect its functional role in viral pathogenesis and COVID-19.
Journal ArticleDOI
Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review
Muhammad Azharuddin Azali,Salma Osman Salih Mohamed,Azian Harun,Faezahtul Arbaeyah Hussain,Shaharum Shamsuddin,Muhammad Farid Johan +5 more
TL;DR: The most cloned SARS-CoV-2 protein is the spike protein this paper , which plays a critical role in SARS co-virus infection and is available in its whole length or as subunits like S1 or the receptor-binding domain (RBD).
Journal ArticleDOI
Production and Characterization of a SARS-CoV-2 Nucleocapsid Protein Reference Material
Bradley B. Stocks,Marie-Pier Thibeault,Denis L'Abbé,Matthew Stuible,Yves Durocher,Jeremy E. Melanson +5 more
TL;DR: In this paper , the National Research Council Canada has developed a SARS-CoV-2 nucleocapsid protein reference material solution, NCAP-1, which is used for rapid antigen tests.
Posted ContentDOI
Expression and novel alternative purification of the recombinant nucleocapsid (N) protein of SARS-CoV-2 in Escherichia coli for the serodiagnosis of COVID-19
Jose David Rosales,William Lobo Quintero,Jhon Cruz,Balbino Perdomo,Militza Quintero,Marcos Bastidas,Jose Domingo Lugo,Keila Rivas Rodriguez,Juan Carlos Freites-Perez,Annie Castillo +9 more
TL;DR: In this paper, the SARS-CoV-2 coronavirus was used to detect the antibodies present in patients using quantitative techniques such as enzyme-linked immunosorbent assay (ELISA) or qualitative rapid tests such as lateral flow chromatography (RDT9s).
References
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Viral targets for vaccines against COVID-19.
Lianpan Dai,George F. Gao +1 more
TL;DR: In this article, the authors discuss which viral elements are used in COVID-19 vaccine candidates, why they might act as good targets for the immune system and the implications for protective immunity.
Patent
Baculovirus expression vectors
TL;DR: In this paper, a method and composition for improving the level of expression of a gene or collection of genes by providing a novel baculovirus promoter to which a heterologous gene is attached is presented.
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The ORF6, ORF8 and nucleocapsid proteins of SARS-CoV-2 inhibit type I interferon signaling pathway.
TL;DR: Screening the viral proteins of SARS-CoV-2 found that the viral ORF6, ORF8 and nucleocapsid proteins were potential inhibitors of type I interferon signaling pathway, a key component for antiviral response of host innate immune.
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Sensitivity in Detection of Antibodies to Nucleocapsid and Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus 2 in Patients With Coronavirus Disease 2019.
Peter D. Burbelo,Francis X. Riedo,Chihiro Morishima,Stephen A. Rawlings,Davey M. Smith,Sanchita Das,Jeffrey R Strich,Daniel S. Chertow,Richard T. Davey,Jeffrey I. Cohen +9 more
TL;DR: Analysis of heat-inactivated samples with a luciferase immunoprecipitation system assay is a safe and sensitive method for detecting SARS-CoV-2 antibodies, which are more sensitive than spike protein antibody for detecting early infection.
Journal ArticleDOI
Lessons for COVID-19 Immunity from Other Coronavirus Infections.
Alan Sariol,Stanley Perlman +1 more
TL;DR: Insight into coronavirus immunity can be informed by previous studies of immune responses to non-human coronaviruses, to common cold coronavIRuses, and to SARS-CoV and MERS- coV, as well as by knowledge of what constitutes a protective immune response and also features that might be pathogenic.