Open AccessJournal Article
An evaluation of the fungal 'morphotype' concept based on ribosomal DNA sequences
DC Lacap,Kevin D. Hyde,Ecy Liew +2 more
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TLDR
This study verifies on the basis of ribosomal DNA sequence analysis the validity of these ‘morphotypes’ as taxonomic groups as well as relatedness of the morphotypes and reference taxa from GenBank.Abstract:
In studies of fungal endophyte communities, mycelia sterilia are commonly isolated from plant substrates and grouped into morphotypes on the basis of cultural characteristics. In Polygonum multiflorum one hundred and sixty-nine strains of mycelia sterilia were isolated and grouped into 27 morphotypes. Six randomly selected morphotypes, each with 2-3 representatives, were subsequently subjected to ribosomal DNA sequence analysis. Nucleotide sequence similarities of the rDNA internal transcribed spacer (ITS) regions and the 5.8S gene were compared using UPGMA cluster analysis. Comparison of nucleotide sequences revealed high levels of similarity (ca. 91.63-99.53%) among strains within morphotypes. ITS and 5.8S sequences of species within various genera from GenBank were obtained to estimate levels of nucleotide similarity within and between well-established genera and species. This study verifies on the basis of ribosomal DNA sequence analysis the validity of these ‘morphotypes’ as taxonomic groups. A dendogram, illustrating relatedness of the morphotypes and reference taxa from GenBank is also presented.read more
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Fungal endophytes limit pathogen damage in a tropical tree
A. Elizabeth Arnold,Luis C. Mejía,Damond Kyllo,Enith I. Rojas,Zuleyka Maynard,Nancy Robbins,Edward Allen Herre +6 more
TL;DR: It is shown that inoculation of endophyte-free leaves with endophytes isolated frequently from naturally infected, asymptomatic hosts significantly decreases both leaf necrosis and leaf mortality when T. cacao seedlings are challenged with a major pathogen.
Journal ArticleDOI
Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR
TL;DR: Endophytic fungi in asymptomatic foliage of loblolly pine (Pinus taeda) in North Carolina, U.S.A., are examined to evaluate morphotaxa, BLAST matches and groups based on sequence similarity as functional taxonomic units, and the utility of PD relative to traditional ecological indices is investigated.
Journal ArticleDOI
A Phylogenetic Evaluation of Whether Endophytes Become Saprotrophs at Host Senescence
Itthayakorn Promputtha,Saisamorn Lumyong,Vijaykrishna Dhanasekaran,Eric H. C. McKenzie,Kevin D. Hyde,Rajesh Jeewon +5 more
TL;DR: Evidence is provided to suggest that some endophytic species change their ecological strategies and adopt a saprotrophic lifestyle upon host decay, and molecular data suggest there are fungal taxa that possibly exist as endophytes and saprotrophs.
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Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru
Romina Gazis,Priscila Chaverri +1 more
TL;DR: Comparisons between leaves and sapwood demonstrated a spatial heterogeneity in endophyte assemblages among plant parts and sites, with a greater diversity of endophytes found in sapwood than in leaves.
References
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TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.
Journal ArticleDOI
A rapid DNA isolation procedure for small quantities of fresh leaf tissue
Edwin Pahlich,Chr. Gerlitz +1 more
TL;DR: From the kinetic data, it becomes evident that the reductive amination reaction is highly adaptive to the ammonium environment.
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PCR protocols : A guide to methods and applications
TL;DR: Basic Methodology: M.A. Innis and D.F. Frohman, RACE: Rapid Amplification of cDNA Ends, and RNA Processing: Apo-B.R. Kwok, Procedure to Minimuze PCR-Product Carry-Over.