Proceedings ArticleDOI
An Improved System for Quantitative Immunoassay Measurement in ImageQuant
TLDR
ImageQuant Analyzer's evolved hardware and software algorithm is described and improvements in TSH analyte from human sera samples were analyzed to validate improvements.Abstract:
ImageQuant Analyzer is an affordable, compact lateral flow immunoassay (LFIA) based PoC diagnostic reader capable of fast quantitative evaluation of analyte concentration from a sample. In this paper, ImageQuant's evolved hardware and software algorithm is described. To validate improvements, TSH analyte from human sera samples were analyzed. Correlation plots between proposed measurements and a Gold Standard reference show superiority of our system (R = 0.96) in predicting TSH concentrations. Validation results for test trials within a single instrument and across instruments (intra-variability < 2 % and inter-variability < 5.5 %) also signify the merits of the newly improved algorithm.read more
Citations
More filters
Journal ArticleDOI
A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I.
TL;DR: In this article, a lateral flow assay (LFA) was used for the quantitative, fluorescence-based detection of the cardiac biomarker troponin I (cTnI) that features an analytical strip made of cellulose filter paper.
Journal ArticleDOI
Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker.
TL;DR: In this article, a rapid, quantitative immunoassay for the detection of cystatin-C was proposed, where a fluorescence-based lateral flow kit was developed in a sandwich format by using a monoclonal antibody.
Journal ArticleDOI
Exploring carbohydrate binding module fusions and Fab fragments in a cellulose-based lateral flow immunoassay for detection of cystatin C
TL;DR: In this paper , a lateral flow assay (LFA) was proposed for the quantitative, fluorescence-based detection of the kidney biomarker cystatin C that features conjugates of capture antibodies and fusions of carbohydrate binding modules (CBM) with ZZ domains anchored on cellulose deposited over nitrocellulose.
References
More filters
Journal ArticleDOI
Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey.
TL;DR: The potentials and limitations of analysis with lateral flow (immuno)assays are illustrated using a literature survey and a SWOT analysis (acronym for “strengths, weaknesses, opportunities, threats”).
Journal ArticleDOI
Integrated rapid-diagnostic-test reader platform on a cellphone.
TL;DR: Providing real-time spatio-temporal statistics for the prevalence of various infectious diseases, this smart RDT reader platform running on cellphones might assist healthcare professionals and policymakers to track emerging epidemics worldwide and help epidemic preparedness.
BookDOI
Lateral flow immunoassay
Raphael C. Wong,Harley Y. Tse +1 more
TL;DR: Manufacturing the Next Generation of Highly Sensitive and Reproducible Lateral Flow Immunoassay Tests and Quantitative, False Positive, and False Negative Issues for Lateral flow immunoassays as Exemplified by Onsite Drug Screens are studied.
Journal ArticleDOI
Cell-phone-based measurement of TSH using Mie scatter optimized lateral flow assays.
TL;DR: An optical system and image analysis algorithm based upon a cell phone platform able to provide point-of-care quantitative TSH results with a high level of sensitivity and reproducibility comparable to that of a clinical laboratory-based third-generation TSH immunoassay is developed.