Journal ArticleDOI
An in situ hybridization protocol for detection and identification of planktonic bacteria
Frank Oliver Glöckner,Rudolf Amann,Albin Alfreider,Jakob Pernthaler,Roland Psenner,Karl-Heinz Trebesius,Karl-Heinz Schleifer +6 more
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TLDR
In this article, the fraction of planktonic bacteria in oligo- and mesotrophic lakes that can be classified by in situ hybridization has been significantly increased with an optimized protocol.About:
This article is published in Systematic and Applied Microbiology.The article was published on 1996-10-01. It has received 327 citations till now.read more
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The Domain-specific Probe EUB338 is Insufficient for the Detection of all Bacteria: Development and Evaluation of a more Comprehensive Probe Set
TL;DR: Two supplementary versions of probe EUB338 are designed and evaluated for in situ detection of most of those phyla not detected with this probe, which should allow a more accurate quantification of members of the domain Bacteria in future molecular ecological studies.
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SAR11 clade dominates ocean surface bacterioplankton communities
Robert M. Morris,Michael S. Rappé,Stephanie A. Connon,Kevin L. Vergin,William A. Siebold,Craig A. Carlson,Stephen J. Giovannoni +6 more
TL;DR: Quantitative measurements of the cellular abundance of the SAR11 clade in northwestern Sargasso Sea waters to 3,000 m and in Oregon coastal surface waters support the conclusion that this microbial group is among the most successful organisms on Earth.
Journal ArticleDOI
Fluorescence In Situ Hybridization and Catalyzed Reporter Deposition for the Identification of Marine Bacteria
TL;DR: The enhanced fluorescence intensities and signal-to-background ratios make CARD-FISH superior to FISH with directly labeled oligonucleotides for the staining of bacteria with low rRNA content in the marine environment.
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Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage
Mohamed F. Haroon,Shihu Hu,Ying Shi,Michael Imelfort,Jurg Keller,Philip Hugenholtz,Zhiguo Yuan,Gene W. Tyson +7 more
TL;DR: Metagenomic, single-cell genomic and metatranscriptomic analyses combined with bioreactor performance and 13C- and 15N-labelling experiments show that ANME-2d is capable of independent AOM through reverse methanogenesis using nitrate as the terminal electron acceptor and Comparative analyses reveal that the genes for nitrate reduction were transferred laterally from a bacterial donor, suggesting selection for this novel process within ANME -2d.
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Cultivation of the ubiquitous SAR11 marine bacterioplankton clade
TL;DR: The isolation of representatives of the SAR11 clade is reported, which indicates that diverse uncultivated microbial taxa dominate most natural ecosystems, which has prompted widespread efforts to elucidate the geochemical activities of these organisms without the benefit of cultures for study.
References
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A modified single solution method for the determination of phosphate in natural waters
J. Murphy,J.P. Riley +1 more
TL;DR: In this article, a single solution reagent was described for the determination of phosphorus in sea water, which consists of an acidified solution of ammonium molybdate containing ascorbic acid and a small amount of antimony.
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Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
TL;DR: Phylogenetic analysis of the retrieved rRNA sequence of an uncultured microorganism reveals its closest culturable relatives and may, together with information on the physicochemical conditions of its natural habitat, facilitate more directed cultivation attempts.
UseofNuclepore Filters forCounting Bacteria by Fluorescence Microscopy
TL;DR: Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter.
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Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
TL;DR: Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry and the intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.