The use of DAPI for identifying and counting aquatic microflora1
Karen G. Porter,Yvette S. Feig +1 more
TLDR
Use of DAPI improved visualization and counting of <1-µm bacteria and blue-green algae in seston-rich samples and extended sample storage to at least 24 weeks.Abstract:
A highly specific and sensitive fluorescing DNA stain, 4′6-diamidino-2-phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1-µm bacteria and blue-green algae in seston-rich samples and extended sample storage to at least 24 weeks.read more
Citations
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Journal ArticleDOI
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
TL;DR: Phylogenetic analysis of the retrieved rRNA sequence of an uncultured microorganism reveals its closest culturable relatives and may, together with information on the physicochemical conditions of its natural habitat, facilitate more directed cultivation attempts.
Journal ArticleDOI
The Ecological Role of Water-Column Microbes in the Sea*
TL;DR: Evidence is presented to suggest that numbers of free bacteria are controlled by nanoplankton~c heterotrophic flagellates which are ubiquitous in the marine water column, thus providing the means for returning some energy from the 'microbial loop' to the conventional planktonic food chain.
Journal ArticleDOI
Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
TL;DR: Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry and the intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.
Journal ArticleDOI
Microbial diversity in the deep sea and the underexplored “rare biosphere”
Mitchell L. Sogin,Hilary G. Morrison,Julie A. Huber,David B. Mark Welch,Susan M. Huse,Phillip R. Neal,Jesús M. Arrieta,Gerhard J. Herndl +7 more
TL;DR: It is shown that bacterial communities of deep water masses of the North Atlantic and diffuse flow hydrothermal vents are one to two orders of magnitude more complex than previously reported for any microbial environment.
Journal ArticleDOI
Extraction of extracellular polymers from activated sludge using a cation exchange resin
TL;DR: In this article, the extraction of water soluble extracellular polymeric substances (EPS) from activated sludge was investigated, which consisted mainly of protein but also humic compounds, carbohydrates, uronic acids and DNA.
References
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Journal ArticleDOI
Use of nuclepore filters for counting bacteria by fluorescence microscopy.
TL;DR: Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter.
Journal ArticleDOI
Determination of bacterial number and biomass in the marine environment.
TL;DR: The biomass of gram-negative (LPS containing) bacteria was shown to be related to the LPS content of the samples, and a factor of 6.35 was determined for converting LPS to bacterial carbon.
Book ChapterDOI
The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA.
D H Williamson,D J Fennell +1 more
TL;DR: The use of 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) in cesium chloride gradients, fluorescent staining of cells with DAPI, and sensitivity of the staining procedure are discussed.
Journal ArticleDOI
Direct counts of aquatic bacteria by a modified epifluorescence technique1
Ralph J. Daley,John E. Hobbie +1 more
Journal ArticleDOI
Acridine orange-epifluorescence technique for counting bacteria in natural waters.
TL;DR: Epifluorescence counting is the method of choice for ecological studies of the natural distribution of bacteria in aquatic environments since it permits ready discrimination of bacteria from detritus and does not rely on the adequacy of culture methods to elicit growth of all viable organisms.