Analysis of variation in isolates of Rhizoctonia solani by random amplified polymorphic DNA assay
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TLDR
Genetic variation in Australian isolates of Rhizoctonia solani was analyzed by random amplified polymorphic DNA (RAPD) assay and fingerprint patterns were generated for each isolate, showing considerable variation in the fingerprint patterns between isolates.About:
This article is published in Fungal Biology.The article was published on 1993-09-01 and is currently open access. It has received 124 citations till now. The article focuses on the topics: RAPD & Rhizoctonia solani.read more
Citations
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Journal ArticleDOI
Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani
TL;DR: Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.
Journal ArticleDOI
Review. Biology and systematics of the form genus Rhizoctonia
TL;DR: This review focuses on the knowledge of several aspects of the species of Rhizcotonia s.
Journal ArticleDOI
Population Biology of the Rhizoctonia solani Complex.
Marc A. Cubeta,Rytas Vilgalys +1 more
Journal ArticleDOI
Development of conventional and quantitative real‐time PCR assays for the detection and identification of Rhizoctonia solani AG‐3 in potato and soil
TL;DR: The amplification of Rhizoctonia solani by seed baiting increased the sensitivity of the assay compared with direct extraction of DNA from the soil, and AG-3 was detectable in artificially inoculated and naturally infested soils when seed Baiting was combined with either the conventional PCR or the real-time PCR assay.
Journal ArticleDOI
The advancing identification and classification of Rhizoctonia spp. using molecular and biotechnological methods compared with the classical anastomosis grouping
TL;DR: Currently, the rDNA-internal transcribed spacer (ITS) sequence analysis seems to be the most appropriate method for classification of Rhizoctonia spp.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
Mathematical model for studying genetic variation in terms of restriction endonucleases
Masatoshi Nei,Wen-Hsiung Li +1 more
TL;DR: A mathematical model for the evolutionary change of restriction sites in mitochondrial DNA is developed and a measure called "nucleotide diversity" is proposed to express the degree of polymorphism in a population at the nucleotide level.
Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Journal ArticleDOI
Rapid preparation of DNA from filamentous fungi
U. Raeder,P. Broda +1 more
TL;DR: A general, simple and inexpensive method for the isolation of DNA from filamentous fungi, starting from freeze‐dried mycelium 01–015% by weight, which allows the processing of many samples in parallel.