Anti-mosquito midgut antibodies block development of plasmodium falciparum and plasmodium vivax in multiple species of anopheles mosquitoes and reduce vector fecundity and survivorship
Altaf A. Lal,Pamela S. Patterson,John B. Sacci,Jefferson A. Vaughan,Chris Paul,William E. Collins,Robert A. Wirtz,Abdu F. Azad +7 more
TLDR
The results reveal that mosquito midgut-based antibodies have the potential to reduce malaria transmission in a synergistic manner by lowering both vector competence, through transmission-blocking effects on parasite development, and vector abundance, by decreasing mosquito survivorship and egg laying capacity.Abstract:
The mosquito midgut plays a central role in the sporogonic development of malaria parasites. We have found that polyclonal sera, produced against mosquito midguts, blocked the passage of Plasmodium falciparum ookinetes across the midgut, leading to a significant reduction of infections in mosquitoes. Anti-midgut mAbs were produced that display broad-spectrum activity, blocking parasite development of both P. falciparum and Plasmodium vivax parasites in five different species of mosquitoes. In addition to their parasite transmission-blocking activity, these mAbs also reduced mosquito survivorship and fecundity. These results reveal that mosquito midgut-based antibodies have the potential to reduce malaria transmission in a synergistic manner by lowering both vector competence, through transmission-blocking effects on parasite development, and vector abundance, by decreasing mosquito survivorship and egg laying capacity. Because the intervention can block transmission of different malaria parasite species in various species of mosquitoes, vaccines against such midgut receptors may block malaria transmission worldwide.read more
Citations
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Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library
Anil K. Ghosh,Paulo Eduardo Martins Ribolla,Paulo Eduardo Martins Ribolla,Marcelo Jacobs-Lorena +3 more
TL;DR: A 12-aa peptide is identified that binds to the distal lobes of the salivary gland and to the luminal side of the midgut epithelium, but not to the midGut surface facing the hemolymph or to ovaries, which suggested that the parasite and peptide recognize the same surface ligand.
Journal ArticleDOI
The Plasmodium bottleneck: malaria parasite losses in the mosquito vector.
TL;DR: The parasite life cycle in the vector is reviewed and human and mosquito contributions that limit malaria parasite development in the mosquito host are highlighted.
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Disruption of Plasmodium falciparum development by antibodies against a conserved mosquito midgut antigen.
Rhoel R. Dinglasan,Dario E. Kalume,Dario E. Kalume,Stefan M. Kanzok,Anil K. Ghosh,Olga Muratova,Akhilesh Pandey,Marcelo Jacobs-Lorena +7 more
TL;DR: It is hypothesized that ookinetes can evade inhibition by two potent transmission-blocking molecules, presumably through the use of other ligands, and that this process further partitions murine from human parasite midgut invasion models.
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An antivector vaccine protects against a lethal vector-borne pathogen.
Milan Labuda,A. R. Trimnell,Martina Ličková,Mária Kazimírová,Gillian M. Davies,Olga Lissina,Rosie S. Hails,Patricia A. Nuttall +7 more
TL;DR: The 64TRP vaccine demonstrates the potential to control vector-borne disease by interfering with pathogen transmission, apparently by mediating a local cutaneous inflammatory immune response at the tick-feeding site.
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Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis.
TL;DR: Mass spectrometry analysis of the proteins found only in blood-fed female midguts, together with data from the recent sequencing of the An.
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