Journal ArticleDOI
Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview.
Frans C. S. Ramaekers,J.J.G. Puts,O. Moesker,A. Kant,A. Huysmans,D. Haag,Paul H.K. Jap,C. J. Herman,G. P. Vooijs +8 more
TLDR
Intermediate-sized filament proteins (IFP) are tissue specific in that antibodies to keratin, vimentin, desmin, glial fibrillary acidic protein (GFAP) and the neurofilament proteins can distinguish between cells of epithelial and mesenchymal origin as well as of myogenic and neural origin respectively.Abstract:
Intermediate-sized filament proteins (IFP) are tissue specific in that antibodies to keratin, vimentin, desmin, glial fibrillary acidic protein (GFAP) and the neurofilament proteins can distinguish between cells of epithelial and mesenchymal origin as well as of myogenic and neural origin respectively. Malignant cells retain their tissue-specific IFP, which makes it possible to use these antibodies in tumour diagnosis. Carcinomas are exclusively detected by antibodies to keratin. Monoclonal antibodies to keratin have allowed the differentiation between subgroups of epithelial tumours until now between adenocarcinomas and squamous cell carcinomas. Lymphomas, melanomas and several soft tissue tumours are distinctly recognized by antibodies to vimentin. On the other hand, rhabdomyosarcomas and leiomyosarcomas are positive for desmin, while astrocytomas give a strong reaction with GFAP antibodies. Thus, antibodies to IFP are useful tools for differential diagnosis in surgical pathology.read more
Citations
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Small Cell Lung Cancer
George R. Simon,Henry N. Wagner +1 more
TL;DR: Evidence-based guidelines for the staging and treatment of SCLC are outlined and it is suggested that patients achieving a complete remission should be offered prophylactic cranial irradiation.
Journal ArticleDOI
Use of monospecific antisera and cRNA probes to localize the major changes in keratin expression during normal and abnormal epidermal differentiation
TL;DR: Three antisera are isolation and characterization that suggest that the basal-specific keratin mRNAs are down-regulated upon commitment to terminal differentiation, but their encoded proteins are stable, and can be detected throughout the spinous layers of the epidermis.
Journal ArticleDOI
Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization.
TL;DR: Interestingly, K13 expression correlated well with the gradient of retinoid-mediated disruptions of intercellular interactions in the culture, suggesting that K13 induction may in some way relate to the reduction in either the number or the strength of desmosomal contacts between suprabasal cells of stratified squamous epithelial tissues.
Journal Article
Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues.
TL;DR: HEA125 is a useful reagent for the distinction of carcinomas from nonepithelial neoplasms, even at very low degrees of histological differentiation, and allows the immunohistochemical detection of micrometastases originating from carcinomas.
Journal ArticleDOI
Keratin Antigens in Differentiating Skin
TL;DR: The changes in expression of intermediate filament proteins that take place during embryological development provide the most potent argument for a central role of intermediate filaments in differentiation.
References
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Journal ArticleDOI
Different intermediate-sized filaments distinguished by immunofluorescence microscopy
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