Journal ArticleDOI
Production of monoclonal antibodies: strategy and tactics.
TLDR
A myeloma line has been developed which produces no globulin chains of its own, has a duplication of 8.7 h, fuses effectively with B-lymphoblasts and produces stable hybrids, and an enhancing effect of macrophages on hybridoma yields has been observed.About:
This article is published in Journal of Immunological Methods.The article was published on 1980-07-15. It has received 1609 citations till now.read more
Citations
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Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
TL;DR: In this article, six monoclonal antibodies have been isolated from mice immunized with synthetic peptide immunogens whose sequences are derived from that of the human c-myc gene product.
Journal Article
The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
TL;DR: In this article, a set of three monoclonal antibodies was described, each of which recognizes cells of the monocyte-macrophage lineage in the rat, and the tissue distribution, in particular in lymphoid organs, was determined by immunoenzyme histochemistry on cryostat sections, as well as on cell suspensions.
Journal ArticleDOI
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
TL;DR: Double immunofluorescent studies carried out with anti-alpha sm-1 and anti- desmin antibodies in several organs revealed a heterogeneity of stromal cells.
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Identification of ZO-1 : a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia
TL;DR: Immunoblot analysis of Madin-Darby canine kidney cells demonstrates the presence of a polypeptide similar in molecular weight to that detected in liver, suggesting that this protein is potentially a ubiquitous component of all mammalian tight junctions.
Journal ArticleDOI
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes
TL;DR: It is proposed that this tissue-specific enhancer contributes to the activation of somatically rearranged immunoglobulin variable region genes and possibly to abnormal expression of other genes (e.g. c-myc) that become translocated to its domain of influence.
References
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Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of basic as well as acidic proteins
TL;DR: This paper describes an alternate procedure for the first dimension which, unlike isoelectric focusing, resolves basic as well as acidic proteins, and involves a short time of electrophoresis toward the cathode and separates most proteins according to their isoelection points.
Journal ArticleDOI
Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion
G. Köhler,C. Milstein +1 more
TL;DR: Cell fusion techniques have been used to produce hybrids between myeloma cells and antibody‐producing cells that are permanently adapted to grow in tissue culture and are capable of inducing antibody-producing tumors in mice.
Journal ArticleDOI
Antibodies to major histocompatibility antigens produced by hybrid cell lines.
TL;DR: FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies.
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Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more