Arabidopsis transcriptional repressor VAL1 triggers Polycomb silencing at FLC during vernalization
TLDR
A single point mutation is identified at an intragenic nucleation site within FLC that prevents a cold-dependent epigenetic switch from taking place in plant homeodomain–Polycomb repressive complex 2 (PHD-PRC2) and indicates a role for the transcriptional repressor VAL1 in the silencing mechanism.Abstract:
The determinants that specify the genomic targets of Polycomb silencing complexes are still unclear. Polycomb silencing of Arabidopsis FLOWERING LOCUS C ( FLC ) accelerates flowering and involves a cold-dependent epigenetic switch. Here we identify a single point mutation at an intragenic nucleation site within FLC that prevents this epigenetic switch from taking place. The mutation blocks nucleation of plant homeodomain–Polycomb repressive complex 2 (PHD-PRC2) and indicates a role for the transcriptional repressor VAL1 in the silencing mechanism. VAL1 localizes to the nucleation region in vivo, promoting histone deacetylation and FLC transcriptional silencing, and interacts with components of the conserved apoptosis- and splicing-associated protein (ASAP) complex. Sequence-specific targeting of transcriptional repressors thus recruits the machinery for PHD-PRC2 nucleation and epigenetic silencing.read more
Citations
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Epigenetic and chromatin-based mechanisms in environmental stress adaptation and stress memory in plants.
Jörn Lämke,Isabel Bäurle +1 more
TL;DR: In this review, both the involvement of chromatin in stress responses and the current evidence on somatic, intergenerational, and transgenerational stress memory are discussed.
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Molecular Regulation of Plant Responses to Environmental Temperatures
TL;DR: Recent progress is discussed in exploring thermomorphogenesis, vernalization, and the mechanisms underlying plant tolerance to extreme temperatures, which are crucial for understanding the interactions between plants and temperature.
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The FLC Locus: A Platform for Discoveries in Epigenetics and Adaptation
Charles Whittaker,Caroline Dean +1 more
TL;DR: Molecular analysis of vernalization pathways has revealed important insight into antisense-mediated chromatin silencing mechanisms that regulate Arabidopsis thaliana FLOWERING LOCUS C, and such insight has enabled molecular dissection of the diversity in vernALization across natural populations of A.Thaliana.
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Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 in Arabidopsis
Jun Xiao,Run Jin,Xiang Yu,Max W. Shen,John D. Wagner,Armaan Pai,Claire Song,Michael Zhuang,Samantha Klasfeld,Chongsheng He,Alexandre M Santos,Chris A. Helliwell,Jose L. Pruneda-Paz,Steve A. Kay,Xiaowei Lin,Sujuan Cui,Meilin Fernandez Garcia,Oliver Clarenz,Justin Goodrich,Xiaoyu Zhang,Ryan S. Austin,Roberto Bonasio,Doris Wagner +22 more
TL;DR: Short genomic fragments, known as Polycomb response elements (PREs), that direct Polycomb repressive complex 2 (PRC2) placement at developmental genes regulated by silencing in Arabidopsis thaliana are defined.
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H3K27 methylation: a promiscuous repressive chromatin mark
TL;DR: Emerging information supports the idea that the chromatin context-including histone modifications, DNA methylation, transcription, chromatin structure and organization within the nucleus-informs PRC2 target selection.
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Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3
Sibum Sung,Richard M. Amasino +1 more
TL;DR: This work identifies a gene with a function in the measurement of the duration of cold exposure and in the establishment of the vernalized state of Arabidopsis thaliana and shows that this silencing involves changes in the modification of histones in FLC chromatin.
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