Journal ArticleDOI
Assay of yeast enzymes in situ. A potential tool in regulation studies.
TLDR
A permeabilization method which allows the assay of several intracellular enzymes within the boundaries of the yeast cell wall is described and the kinetic properties of hexokinase and pyruvate kinase examined in the permeabilized cells are essentially the same as in cell-free extracts.Abstract:
A permeabilization method which allows the assay of several intracellular enzymes within the boundaries of the yeast cell wall is described. The kinetic properties of hexokinase and pyruvate kinase examined in the permeabilized cells, including the allosteric activation of the latter by fructose bisphosphate, are essentially the same as in cell-free extracts.read more
Citations
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Journal ArticleDOI
Regulation of trehalose mobilization in fungi.
TL;DR: The inhibition of regulatory trehalase by nitrogen sources in the presence of glucose and the induction of fungal spore germination by heat shock are studied.
Journal ArticleDOI
A Biochemical Explanation for Lipid Accumulation in Candida 107 and Other Oleaginous Micro-organisms
Philip A. Botham,Colin Ratledge +1 more
TL;DR: The biochemical explanation for lipid accumulation was investigated principally in Candida 107 and, for comparison, in the non-oleaginous yeast Candida utilis and there were no significant differences between these two yeasts in their control of glucose uptake.
Journal ArticleDOI
Characterization of the plasma membrane ATPase of Saccharomyces cerevisiae.
TL;DR: The distribution of ATPase and several marker enzymes was examined after differential and sucrose gradient centrifugation of yeast homogenates and it is suggested that the active site is on the cytoplasmic side of the plasma membrane.
Journal ArticleDOI
Glycolysis mutants in Saccharomyces cerevisiae.
TL;DR: Mutants have been isolated in S. cerevisiae with the phenotype of growth on pyruvate but not on glucose, or growth on rich medium with pyruVate but inhibition by glucose, and double mutants now also lacking hexokinase, phosphofructokinase or several enzymes of glycolysis.
Journal ArticleDOI
Permeabilization of microorganisms by Triton X-100.
TL;DR: A simple permeabilization procedure has been developed which allows the reliable determination of enzyme activitiesin situ in a variety of different microorganisms and has been successfully applied to the determination of enzymes activities in other fungi as well as in gram-positive and gramnegative bacteria.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Fructose-1,6-diphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and non fermenting yeasts.
Juana M. Gancedo,Carlos Gancedo +1 more
TL;DR: A remarkable difference in the sensitivity of the fructose-1,6-diphosphatase from both groups towards inhibition by AMP was observed, while the enzyme from aerobic yeasts showed different responses, inhibition ranging from 10% in Rhodotorula and Sporobolomyces, to 90% in Pichia.
Journal ArticleDOI
A Kinetic Study of Glycolytic Enzyme Synthesis in Yeast
P.K. Maitra,Z. Lobo +1 more
TL;DR: The glucose-induced increase in glycolytic enzyme activity in Saccharomyces cerevisiae is completely prevented by cycloheximide, an inhibitor of protein synthesis in this yeast.
Book ChapterDOI
Concentrations of Metabolites and Binding Sites. Implications in Metabolic Regulation
Alberto Sols,R. Marco +1 more
TL;DR: This chapter discusses the implications in metabolic regulation because of the concentrations of metabolites and binding sites in the eukaryotic cell of higher organisms, in which practically all important metabolic reactions occur.
Journal ArticleDOI
Enzyme concentrations in tissues.
TL;DR: It is pointed out that these concentrations are much higher than those used in enzymatic studies in vitro, and metabolic interpretations of experiments in vitro should consider this additional departure from conditions in vivo.