Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media
G.A. Broderick,J.H. Kang +1 more
TLDR
Catalyzed phenol-hypochlorite and ninhydrin colorimetric procedures were adapted to the Technicon AutoAnalyzer for simultaneous determination of ammonia and total amino acids in ruminal fluid or ruminal in vitro media and indicated high degrees of accuracy and precision for both ammonia and amino acid analyses.About:
This article is published in Journal of Dairy Science.The article was published on 1980-01-01 and is currently open access. It has received 1806 citations till now. The article focuses on the topics: Ninhydrin & Amino acid.read more
Citations
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Effect of replacing alfalfa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives
TL;DR: Twenty-four multiparous dairy cows were blocked by days in milk and assigned to six balanced 4 x 4 Latin squares with 21-d periods, suggesting that this was the optimal level for utilization of dietary NPN from alfalfa silage and other sources.
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Applications of the Ninhydrin Reaction for Analysis of Amino Acids, Peptides, and Proteins to Agricultural and Biomedical Sciences
TL;DR: This review attempts to integrate and correlate the widely scattered literature on ninhydrin reactions of a variety of structurally different compounds and provides a scientific basis for further improvements of this important analytical technique.
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Effect of increasing degradable intake protein on intake and digestion of low-quality, tallgrass-prairie forage by beef cows
TL;DR: Monitoring intake, ruminal fermentation responses, and site and extent of digestion associated with providing increasing amounts of supplemental degradable intake protein (DIP) generally improved forage utilization.
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Structural Growth, Rumen Development, and Metabolic and Immune Responses of Holstein Male Calves Fed Milk Through Step-Down and Conventional Methods
Muhammad Ajmal Khan,H.J. Lee,W.S. Lee,H.S. Kim,K.S. Ki,T.Y. Hur,Guk-Hyun Suh,S.J. Kang,Yun-Jaie Choi +8 more
TL;DR: Greater feed consumption, BW gain, and structural growth, and a more metabolically and physically developed rumen were observed in calves on the STEP method than in those on the conventional method.
References
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Principles and Procedures of Statistics.
Journal ArticleDOI
Modified Reagents for Determination of Urea and Ammonia
TL;DR: Combinations of reagents are described for the catalyzed indophenol reaction for the determination of ammonia, which produces a stable blue color, and the procedure is adapted to thedetermination of urea after hydrolysis with urease.
Book
Microdiffusion Analysis and Volumetric Error
TL;DR: In this article, Microdiffusion analysis and volumetric error was used to detect micro-diffusion errors in the context of micro-scale analysis of the volumetry data.