Bacteria mature preproinsulin to proinsulin.
TLDR
By inserting the rat preproinsulin gene into the bacterial prepenicillinase gene, a variety of hybrid bacterial-eukaryotic signal sequences attached to proinsulin are formed and it is shown that the bacteria correctly process both the bacterial and the eukaryosis signal sequences of these hybrid proteins.Abstract:
By inserting the rat preproinsulin gene into the bacterial prepenicillinase gene, we formed a variety of hybrid bacterial-eukaryotic signal sequences attached to proinsulin. Among these were the four following constructions: rat proinsulin attached to the entire penicillinase signal sequence and rat preproinsulin fused to all of, to half of, or only to the first four amino acids of the bacterial signal sequence. In all four cases, more than 90% of the rat insulin antigen appeared in the periplasmic space. By immunoprecipitation and determination of the amino acid sequences of the radiolabeled products, we show that the bacteria correctly process both the bacterial and the eukaryotic signal sequences of these hybrid proteins. The cleavage of the eukaryotic signal by bacterial peptidase, in this case, generates proinsulin.read more
Citations
More filters
Journal ArticleDOI
Intracellular protein topogenesis.
TL;DR: A cell contains millions of protein molecules, which are continually being synthesized and degraded, and at homeostasis, a given species of protein is represented by a characteristic number of molecules that is kept constant within a narrow range.
Journal ArticleDOI
Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors
Yosef Yarden,Jaime Escobedo,Wun-Jing Kuang,Teresa L. Yang-Feng,T. O. Daniel,P. M. Tremble,Ellson Y. Chen,M. E. Ando,Richard N. Harkins,Uta Francke,Victor A. Fried,Axel Ullrich,Lewis T. Williams +12 more
TL;DR: The primary structure of the receptor for platelet-derived growth factor (PDGF), determined by means of cloning a cDNA that encodes the murine pre-PDGF receptor, is closely related to that of the v-kit oncogene product and the receptors for macrophage colony stimulating factor (CSF-1).
Journal ArticleDOI
Mechanisms for the incorporation of proteins in membranes and organelles.
TL;DR: Mechanisms for the transfer of newly synthesized polypeptides to their sites of function in different subcellular membranes and organelles are considered, and models in which specific features of the polypePTides serve as signals to direct them along selected sub cellular pathways to their final destination are discussed.
Journal ArticleDOI
The Regulatory Particle of the Saccharomyces cerevisiae Proteasome
TL;DR: Overall, regulatory particles from yeasts and mammals are remarkably similar, suggesting that the specific mechanistic features of the proteasome have been closely conserved over the course of evolution.
Journal ArticleDOI
Protein translocation across membranes
Keith Verner,Gottfried Schatz +1 more
TL;DR: Translocation requires a signal on the protein itself, a loose conformation of the protein, energy, and receptor-like components in the cytosol and on the target membrane.
Related Papers (5)
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.
Peter Walter,Gonter Blobel +1 more