Journal ArticleDOI
BcXTH1, a Brassica campestris homologue of Arabidopsis XTH9, is associated with cell expansion.
Yoon-Kyung Shin,Hyunsik Yum,Eun-Sook Kim,Hongju Cho,Kodiveri Muthukaliannan Gothandam,Jiyoung Hyun,Yong-Yoon Chung +6 more
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TLDR
Investigation of the isolation and characterization of a XTH gene from a pistil cDNA library of Brassica campestris revealed that it is homologous to the XTH9 gene of Arabidopsis, and Immunoelectron microscopy shows that BcXTH1 is localized almost exclusively to the cell wall, supporting the conclusion that it participates in the regulation of cell expansion in B.campestris.Abstract:
Xyloglucan endotransglucosylase/hydrolases (XTHs) are a group of the enzymes that are responsible for reorganization of the cellulose–xyloglucan framework by catalyzing cleavage and religation of the xyloglucan chains in the plant cell wall. In this study, we report the isolation and characterization of a XTH gene from a pistil cDNA library of Brassica campestris. Sequence analysis of the gene, designated BcXTH1, revealed that it is homologous to the XTH9 gene of Arabidopsis. The highly conserved domain (DEIDFEFLG) found among all XTHs was also present in BcXTH1 but with the two amino acid substitutions (NEFDFEFLG) also found in Arabidopsis XTH9. These results suggest that BcXTH1 is the B. campestris homologue of XTH9. Expression analysis of BcXTH1 revealed that it was expressed in most of the plant organs. In situ hybridization showed that the gene is highly expressed in the floral primodia, especially in the epidermal cell layer. Southern blot analysis indicated that the BcXTH1 gene exists as a multi-copy gene in the B. campestris genome. The function of the BcXTH1 gene was deduced from using an overexpression strategy in Arabidopsis. Interestingly, the transgenic plants showed a pronounced cell expansion phenotype. Immunoelectron microscopy shows that BcXTH1 is localized almost exclusively to the cell wall, supporting our conclusion that it participates in the regulation of cell expansion in B. campestris.read more
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Enzymic characterization of two recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis and their effect on root growth and cell wall extension
TL;DR: It is proposed that these specific XTH isoenzymes play a role in strengthening the side-walls of root-hairs and cell walls in the root differentiation zone after the completion of cell expansion.
Journal ArticleDOI
SIZ1 Controls Cell Growth and Plant Development in Arabidopsis Through Salicylic Acid
TL;DR: Evidence is provided that the SIZ1 gene controls cell division and elongation through regulation of the SA level and that XTH8 and XTH31 genes may be responsible for reduced leaf cell expansion.
Journal ArticleDOI
Cell Wall Modifying Proteins Mediate Plant Acclimatization to Biotic and Abiotic Stresses
TL;DR: This review documents and discusses studies demonstrating the importance of timely cell wall modification during plant stress responses by focusing on a well-researched subset of wall modifying proteins.
Journal ArticleDOI
Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation
Joohyun Lee,Teresa H. Burns,Ginger G. Light,Yan Sun,Mohamed Fokar,Yoshihisha Kasukabe,Koichi Fujisawa,Yoshihiko Maekawa,Randy D. Allen +8 more
TL;DR: The function of GhXTH1 in mediating cotton fiber elongation was analyzed in transgenic cotton plants that express a transgene consisting of the Gh XTH1 coding sequence under transcriptional control of the CaMV 35S promoter.
Journal ArticleDOI
On the salty side of life: molecular, physiological and anatomical adaptation and acclimation of trees to extreme habitats
Andrea Polle,Shaoliang Chen +1 more
TL;DR: The molecular, physiological and anatomical adjustments underlying salt tolerance in glycophytic and halophytic model tree species, such as Populus euphratica in terrestrial habitats, and mangrove species along coastlines are reviewed.
References
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Journal ArticleDOI
The gene for a xyloglucan endotransglucosylase/hydrolase from Cicer arietinum is strongly expressed in elongating tissues.
TL;DR: An involvement of the putative XTH encoded by CaXTH1 in the chickpea cell expansion process is suggested, which seems to be specific for growing tissue and also in vascular tissues.
Journal Article
Expression analysis of the pistil genes in controlling self-incompatibility of brassica campestris by CO2 gas using microarray.
TL;DR: The results demonstrate that the overcoming of SI by CO2 gas involves reduced expression of the SLG gene in Brassica, one of the down-regulated genes in the susceptible line.
Journal ArticleDOI
Molecular characterization of an anther-preferential gene from rice
TL;DR: Northern blot analysis revealed no gene expression in vegetative organs, leaves, or roots, however, expression was high in the flowers, especially in the anthers, andRA1003 (detected only in theAnther cDNA library) was selected and further characterized at the molecular level.